Rapid detection of multiple sildenafil and tadalafil adulterants in dietary supplements by dual-labeled probe time-resolved fluorescence immunochromatography assay

Wei, Tian; Zhang, Yongyi; Huang, Jianxin; Yang, Jinyi; Tian, Yuanxin; Shen, Yudong

doi:10.1016/j.fbio.2024.103905

Cited by 3 publications

References 42 publications

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Lateral Flow Immunoassay for the Rapid Detection of Thiamethoxam in Tea Based on a SERS Tag Constructed by Phenolic-Mediated Coating Engineering

Chen,

Xu,

Zhu

et al. 2024

J. Agric. Food Chem.

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Sensitive and accurate detection of thiamethoxam in tea is significant to ensuring consumer health. In this study, surface-enhanced Raman scattering (SERS) tags were prepared by using a polyphenol-mediated coating engineering strategy. This approach involved the self-assembly of tannic acid (TA) and self-polymerization of benzene-1,4-dithiol (BDT) on the surface of gold nanoparticles, resulting in the formation of Au@pBDT-TA. The SERS tags possess high Raman signals and antibody adsorption properties, avoiding the complex decoration or label steps of SERS reporters. We discovered that Au@pBDT-TA, composed of gold nanoparticles at 40 nm and a pBDT-TA thickness of 10 nm, was optimal for the development of the SERS lateral flow immunoassay (LFIA). In comparison to the gold nanoparticle-based LFIA, the SERS−LFIA demonstrated 4-fold and 720-fold enhancements in visual and quantitative limits of detection in buffer solution. The SERS−LFIA demonstrated quantitative limits of detection of 0.06 and 0.1 ng/g for black and green tea, respectively, with a broader linear range spanning over 2 orders of magnitude and a short detection time of 20 min. The proposed SERS−LFIA not only offers a sensitive and reliable method for monitoring thiamethoxam in tea but also possesses a versatile potential that can be easily adapted for the trace detection of various other targets.

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Lateral Flow Immunoassay for the Rapid Detection of Thiamethoxam in Tea Based on a SERS Tag Constructed by Phenolic-Mediated Coating Engineering

Chen,

Xu,

Zhu

et al. 2024

J. Agric. Food Chem.

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Paper-Based Aptasensor Assay for Detection of Food Adulterant Sildenafil

Kavruk,

Ozalp

2024

Biosensors

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Sildenafil is used to treat erectile dysfunction and pulmonary arterial hypertension but is often illicitly added to energy drinks and chocolates. This study introduces a lateral flow strip test using aptamers specific to sildenafil for detecting its illegal presence in food. The process involved using graphene oxide SELEX to identify high-affinity aptamers, which were then converted into molecular gate structures on mesoporous silica nanoparticles, creating a unique signaling system. This system was integrated into lateral flow chromatography strips and tested on buffers and chocolate samples containing sildenafil. The method simplifies the lateral flow assay (LFA) for small molecules and provides a tool for signal amplification. The detection limit for these strips was found to be 68.2 nM (31.8 µg/kg) in spiked food samples.

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Nature’s Impostors: Electrochemical Detection of Sildenafil in Natural Products

J. Castillo

2024

Current Developments in Biosensor Applications and Smart Strategies [Working Title]

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The electrochemical detection of sildenafil, a phosphodiesterase type 5 inhibitor used in the treatment of erectile dysfunction, has been of huge interest to researchers due to the wide prevalence of its adulteration in natural products. This review explores the chemical properties, pharmacological effects, and action mechanism of sildenafil, emphasizing its potent vasodilatory effects mediated through the inhibition of phosphodiesterase type 5. The illicit addition of sildenafil to natural products raises substantial health concerns, including severe cardiovascular events, especially in vulnerable populations. Analytical methods for the detection of sildenafil are critically reviewed, with a focus on chromatographic techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry, which offer high sensitivity and specificity. Electrochemical detection methods, including voltammetry and amperometry, are highlighted for their potential in providing rapid, cost-effective, and on-site analysis. The review discusses the challenges associated with electrochemical detection, such as interference from complex matrices and the need for highly selective electrode materials. Advances in nanomaterials, such as graphene and carbon nanotubes, are explored for their ability to enhance detection sensitivity and selectivity. Future directions include the development of portable electrochemical sensors and integrated analytical platforms for reliable and robust detection of sildenafil in natural products, ensuring consumer safety and regulatory compliance.

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Rapid detection of multiple sildenafil and tadalafil adulterants in dietary supplements by dual-labeled probe time-resolved fluorescence immunochromatography assay

Cited by 3 publications

References 42 publications

Lateral Flow Immunoassay for the Rapid Detection of Thiamethoxam in Tea Based on a SERS Tag Constructed by Phenolic-Mediated Coating Engineering

Lateral Flow Immunoassay for the Rapid Detection of Thiamethoxam in Tea Based on a SERS Tag Constructed by Phenolic-Mediated Coating Engineering

Paper-Based Aptasensor Assay for Detection of Food Adulterant Sildenafil

Nature’s Impostors: Electrochemical Detection of Sildenafil in Natural Products

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