Rapid detection of mumps virus by the polymerase chain reaction

Boriskin, Yu. S.; Booth, James C.; Yamada, Akio

doi:10.1016/0166-0934(93)90173-o

Cited by 28 publications

(1 citation statement)

References 20 publications

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“…A sandwich ELISA with monoclonal antibodies was also reported to be a very effective diagnostic method [10]. Recently, the polymerase chain reaction (PCR) method [17] has been employed widely to detect a number of viruses, such as the human immunodeficiency virus [15], hepatitis B virus [9], bovine viral diarrhea virus [6], infectious bursal disease virus [11], flavivirus [19], mumps virus [1] and others.…”

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African Horsesickness Virus Infection in Adult Mice.

Sakamoto

Kawashima

Narita

et al. 2000

JES

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Adult BALB/c mice were inoculated intranasally with African horsesickness virus serotype 4 (AHSV-4), the attenuated-vaccine strain. The 4-week-old mice died after developing nervous signs at 7 to 8 days post infection (p.i). Lesions, such as perivascular accumulation of lymphocytes and necrosis of neurons, were found only in the brains of virus-infected mice, but not in those of controls. Brains, red blood cells (RBC) and white blood cells (WBC) were collected from two mice each day p.i for virus isolation and RT-PCR. Virus could be isolated from brains collected at 2 to 7 days p.i of infection mice and only one of WBC sample collected on 7 day p.i, but no virus was isolated from any RBC samples. In contrast, RT-PCR products were visible in agarose gel electrophoresis stained with ethidium bromide from RBC and WBC samples collected at days 1 to 7 p.i, and from brain samples collected at days 2 to 7 p.i. The amplified PCR products were increased with days p.i when using extracted RNAs from brain samples, but the quantity of the PCR products was relatively constant with the RBC and WBC samples. It was shown that mice were susceptible to AHSV.

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African Horsesickness Virus Infection in Adult Mice.

Sakamoto

Kawashima

Narita

et al. 2000

JES

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Detection of mumps virus genome directly from clinical samples and a simple method for genetic differentiation of the Hoshino vaccine strain from wild strains of mumps virus

Kashiwagi

Kawashima²,

Takekuma³

et al. 1997

J. Med. Virol.

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A simple and sensitive method was developed for the differentiation of the Hoshino vaccine strain from wild strains with a restriction fragment length polymorphism (RFLP) analysis in the part of hemagglutinin-neuraminidase (HN) gene. The virus genome was amplified by using a reverse transcriptase-polymerase chain reaction (RT-PCR) directly from clinical samples. The PCR product of the Hoshino vaccine strain was cleaved into 2 fragments after digestion with Sca I and Afl II. All wild strains showed 2 RFLP profiles, A and B, different from that of vaccine strain. Wild A strains were cut into 2 fragments after digestion with Sca I only, while wild B strains were cleaved neither with Sca I nor Afl II. This molecular approach provides an effective method for differentiation of the Hoshino vaccine strain from wild strains of mumps virus in patients after vaccination.

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Evaluation of combining upper respiratory tract swab samples with cerebrospinal fluid examination for the diagnosis of enteroviral meningitis in children

et al. 1999

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In a prospective comparative study, the use of combined analysis of upper respiratory tract swab samples and cerebrospinal fluid (CSF) samples was assessed to improve the detection rate of enteroviral meningitis in children. An enterovirus was detected in 32% of patients with aseptic meningitis when testing CSF samples alone compared with 71.5% when combining CSF and respiratory tract findings. An enterovirus was detected in 17% of respiratory tract samples in an age- and sex-matched control group without meningitis. Thus, combining the examination of upper respiratory tract with CSF findings may improve the detection rate of enteroviral meningitis. Upper respiratory tract samples should be included in the diagnosis scheme to differentiate benign enteroviral meningitis from other life-threatening infections of the central nervous system.

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Rapid detection of mumps virus by the polymerase chain reaction

Cited by 28 publications

References 20 publications

African Horsesickness Virus Infection in Adult Mice.

African Horsesickness Virus Infection in Adult Mice.

Detection of mumps virus genome directly from clinical samples and a simple method for genetic differentiation of the Hoshino vaccine strain from wild strains of mumps virus

Evaluation of combining upper respiratory tract swab samples with cerebrospinal fluid examination for the diagnosis of enteroviral meningitis in children

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