Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection

Lin, Ming‐Cheng; Nawarak, Jiraporn; Chen, Tai-Yuan; Tsai, Hsien-Yu; Hsieh, Jung-Feng; Sinchaikul, Supachok; Chen, Shui‐Tein

doi:10.1063/1.3194283

Cited by 19 publications

(9 citation statements)

References 37 publications

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“…The value for the interaction between BNP and antiBNP agrees well with previous reports. 25 The DNA aptamer sequence was recently identified by Lin et al, 26 and this is the first time a binding affinity coefficient has been reported. A detection limit of ∼1 nM shown here is typical for conventional SPR measurements.…”

Section: ■ Experimental Sectionmentioning

confidence: 97%

Ultrasensitive and Ultrawide Range Detection of a Cardiac Biomarker on a Surface Plasmon Resonance Platform

et al. 2013

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One of the main challenges in the development of new analytical platforms for ultrasensitive bioaffinity detection is jointly achieving a wide dynamic range in target analyte concentration, especially for approaches that rely on multistep processes as a part of the signal amplification mechanism. In this paper, a new surface-based sandwich assay is introduced for the direct detection of B-type natriuretic peptide (BNP), an important biomarker for cardiac failure, at concentrations ranging from 1 aM to 500 nM. This was achieved using nanoparticle-enhanced surface plasmon resonance (SPR) where a DNA aptamer is immobilized on a chemically modified gold surface in conjunction with the specific adsorption of antiBNP coated gold nanocubes in the presence of the biomarker target. A concentration detection range greater than eleven orders of magnitude was achieved through dynamic control of only the secondary nanoparticle probe concentration. Furthermore, detection at low attomolar concentrations was also achieved in undiluted human serum.

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Section: ■ Experimental Sectionmentioning

confidence: 97%

Ultrasensitive and Ultrawide Range Detection of a Cardiac Biomarker on a Surface Plasmon Resonance Platform

et al. 2013

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“…Simultaneous determination of angiotensin II and angiotensin‐(1–7) in plasma and urine from normotensive and hypertensive rats, with and without antihypertensive treatment, was possible over the range 0.01–500 pmol/mL within 10 min by CZE with UV‐detection at 200 nm in the BGE composed of 10 mM boric acid, 3 mM tartaric acid, 10 fM AuCl 3 , pH 9.80, at +30 kV in 75 μm id×57/50 cm long FS capillary 216. Rapid determination of brain natriuretic peptide in blood, biomarker for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy, was possible by microfluidic LabChip analyzer using fluorescently labeled aptamers 217.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in CE and CEC of peptides (2009–2011)

Kašička

2011

Electrophoresis

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The review brings a comprehensive survey of the recent developments of high-performance electroseparation methods in capillary and microchip formats: zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography. Applications of these techniques to analysis, isolation, purification and physicochemical and biochemical characterization of peptides are described. Advances in the investigation of electromigration properties of peptides, and in the methodology of their analysis, such as sample preparation, adsorption suppression, EOF control and detection, are presented. New developments, in particular, CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.

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“…Another excellent work using a highly specific and sensitive DNA aptamer, instead of using antibodies, for the brain natriuretic peptide (BNP) assay has been reported by using Agilent lab-on-a-chip (Agilent Technologies, Waldbronn, Germany) technology [ 52 ]. BNP is a neurohormone that causes diuresis, natriuresis and vasodilatation.…”

Section: Aptamer Assays For Clinical Applicationsmentioning

confidence: 99%

Screening of Aptamers on Microfluidic Systems for Clinical Applications

Weng

Huang

Lee

2012

Sensors

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The use of microfluidic systems for screening of aptamers and their biomedical applications are reviewed in this paper. Aptamers with different nucleic acid sequences have been extensively studied and the results demonstrated a strong binding affinity to target molecules such that they can be used as promising candidate biomarkers for diagnosis and therapeutics. Recently, the aptamer screening protocol has been conducted with microfluidic-based devices. Furthermore, aptamer affinity screening by a microfluidic-based method has demonstrated remarkable advantages over competing traditional methods. In this paper, we first reviewed microfluidic systems which demonstrated efficient and rapid screening of a specific aptamer. Then, the clinical applications of screened aptamers, also performed by microfluidic systems, are further reviewed. These automated microfluidic systems can provide advantages over their conventional counterparts including more compactness, faster analysis, less sample/reagent consumption and automation. An aptamer-based compact microfluidic system for diagnosis may even lead to a point-of-care device. The use of microfluidic systems for aptamer screening and diagnosis is expected to continue growing in the near future and may make a substantial impact on biomedical applications.

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Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection

Cited by 19 publications

References 37 publications

Ultrasensitive and Ultrawide Range Detection of a Cardiac Biomarker on a Surface Plasmon Resonance Platform

Ultrasensitive and Ultrawide Range Detection of a Cardiac Biomarker on a Surface Plasmon Resonance Platform

Recent developments in CE and CEC of peptides (2009–2011)

Screening of Aptamers on Microfluidic Systems for Clinical Applications

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