Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay

Kadota, Tomoyuki; Takezawa, Yoko; Hirano, Seishiro; Tajima, O.; Maragos, Chris M.; Nakajima, Takashi; Tanaka, Toshitsugu; Kamata, Yoichi; Sugita-Konishi, Yoshiko

doi:10.1016/j.aca.2010.05.028

Cited by 58 publications

(32 citation statements)

References 34 publications

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“…Eventually, surface plasmon resonance (SPR) has attracted attention as a fast label-free screening method for the detection of food contaminants, in particular, mycotoxins [28–34]. This technique has a number of advantages: SPR is a rapid label-free detection method that gives quantitative and qualitative information of the analyzed samples and provides the possibility to reuse the sensor chip for many analytical cycles [35].…”

Section: Introductionmentioning

confidence: 99%

Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins

Dorokhin

Haasnoot²,

Franssen

et al. 2011

Anal Bioanal Chem

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A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin–protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 μg/kg for DON and 64 and 40 μg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples.

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Section: Introductionmentioning

confidence: 99%

Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins

Dorokhin

Haasnoot²,

Franssen

et al. 2011

Anal Bioanal Chem

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“…The development of antibody-based technologies to detect DON is advancing rapidly and has resulted in many novel technologies being applied. These have included fluorescence polarisation immunoassay (Lippolis, Pascale, & Visconti, 2006;Maragos & Plattner 2002), surface plasmon-based immunoassays (Kadota et al, 2010;Schnerr, Vogel, & Niessen, 2002;Tü dos, Lucas-van den Bos, & Stigter, 2003), array-based devices (Ngundi et al, 2006), flow cytometry (Peters, BienenmannPloum, de Rijk, & Haasnoot, 2011) and biolayer interferometry (Maragos, 2011). In addition, there have been recent advances in the use of antibodies in novel cleanup and isolation technologies including immuno-ultrafiltration, sol-gel immunoaffinity columns and polymer-based solid phase extraction (Bö hm, Cichna-Markl, BrennStruckhofova, & Razzazi-Fazeli, 2008;Brenn-Stuckhofova, Fü reder, Cichna-Markl, & Razzazi-Fazeli, 2009;Pascale et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol

Maragos

Chen

2012

Food and Agricultural Immunology

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Deoxynivalenol (DON) is a mycotoxin produced by certain fungi that infest cereal grains. A hybridoma cell line producing a monoclonal antibody (Mab) was used as the starting point in the development of a recombinant single chain variable fragment antibody (scFv) recognising DON. The scFv and Mab were characterised using two immunoassay formats: competitive direct enzyme-linked immunosorbent assay (CD-ELISA) and biolayer interferometry (BLI). Using CD-ELISA the IC 50 s for DON were 36.1 and 13.8 ng/ml for assays based on the scFv and Mab, respectively. The cross-reactivity to DON analogs was very similar for the scFv and the Mab. The real-time binding of the antibodies to an immobilised DON-protein conjugate was also monitored. In competitive BLI assays the IC 50 s using the scFv and Mab were 68.3 and 15.8 ng/ml, respectively. The results suggest that sensitivity of assays, but not selectivity, was affected by removal of the constant regions of the Mab.

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“…SPR sensing can be used in the competitive inhibition assay and sandwich assay to determine mycotoxins. In competitive inhibition format, mycotoxin–protein conjugates (antigen) can be loaded on the activated SPR chip surface [36,37]. After the injection of sample-antibody mixture solution, the competition can be found between immobilized antigen and free mycotoxin in the sample.…”

Section: Sensing Strategiesmentioning

confidence: 99%

“…Competitive inhibition immunoassay was conducted with SPR for rapid screening of multiple mycotoxins. Via the immobilization mycotoxin-labeled BSA on the sensor chip, Tomoyuki and coworkers [37] fabricated a SPR immunosensor with a simple fabrication within 600 s, including immobilization, activation and blocking. By using a continuous flow microspotter device, a microarray was fabricated and obtained for ZEA and DON sensing in maize and wheat samples [36].…”

Section: Applicationsmentioning

confidence: 99%

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Zhang

et al. 2016

Toxins

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Mycotoxin contamination threatens health and life of humans and animals throughout the food supply chains. Many of the mycotoxins have been proven to be carcinogens, teratogens and mutagens. The reliable and sensitive sensing methods are requested to monitor mycotoxin contamination. Advanced sensors based on antibodies or aptamers boast the advantages of high sensitivity and rapidity, and have been used in the mycotoxin sensing. These sensors are miniaturized, thereby lowering costs, and are applicable to high-throughput modes. In this work, the latest developments in sensing strategies for mycotoxin determination were critically discussed. Optical and electrochemical sensing modes were compared. The sensing methods for single mycotoxin or multiple mycotoxins in food samples were reviewed, along with the challenges and the future of antibody or aptamer-based sensors. This work might promote academic studies and industrial applications for mycotoxin sensing.

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Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay

Cited by 58 publications

References 34 publications

Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins

Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins

Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

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