Rapid detection of pigeon Megrivirus using TaqMan real-time PCR technology

Zhang, Jinpeng; Fu, Haidong; Chen, Cuiteng; Jiang, Jinxiu; Lin, Yu‐Sheng; Jiang, Bin; Lin, Lin; Hu, Qilin; Wan, Chunhe

doi:10.1016/j.psj.2023.103027

Cited by 7 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in accordance with the other research concerning these viruses because the available studies are unfortunately very limited. While Zhang et al were able to identify Megrivirus B in pigeons with diarrhoea, they did not explore the potential association of the presence of the virus with the signs of disease [ 12 ]. The other study on Megrivirus B involved birds of unknown health status, as does the only available study on Pigeon picornavirus B [ 6 , 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…To test the samples for the presence of Megrivirus B genetic material, qPCR was performed with primers Mes2-2F, Mes2-2R and probe Mes2-p using the method developed by Zhang et al [ 12 ]. The reaction mixture consisted of 10 µL of TaqMan™ Fast Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 1.8 µL of forward and reverse primer, 2 µL of probe, 1.4 µL of RNase-free water and 3 µL of cDNA.…”

Section: Methodsmentioning

confidence: 99%

“…Pigeon megriviruses, also called mesiviruses from the Megrivirus sister-clade virus, belong to the Megrivirus B species. They have been detected in faeces of feral pigeons of unknown health status by Phan et al and in faeces of racing pigeons suffering from diarrhoea by Zhang et al [ 6 , 12 ]. Among the picornaviruses infecting pigeons, there are also two species that have not been assigned to any genus, called Pigeon picornavirus A and B , first discovered by Kofstad and Jonassen in feral pigeons of unknown health status [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recombinant Viruses from the Picornaviridae Family Occurring in Racing Pigeons

Łukaszuk,

Dziewulska,

Stenzel

2024

Viruses

View full text Add to dashboard Cite

Viruses from Picornaviridae family are known pathogens of poultry, although the information on their occurrence and pathogenicity in pigeons is scarce. In this research, efforts are made to broaden the knowledge on Megrivirus B and Pigeon picornavirus B prevalence, phylogenetic relationship with other avian picornaviruses and their possible connection with enteric disease in racing pigeons. As a result of Oxford Nanopore Sequencing, five Megrivirus and two pigeon picornavirus B-like genome sequences were recovered, among which three recombinant strains were detected. The recombinant fragments represented an average of 10.9% and 25.5% of the genome length of the Pigeon picornavirus B and Megrivirus B reference strains, respectively. The phylogenetic analysis revealed that pigeons are carriers of species-specific picornaviruses. TaqMan qPCR assays revealed 7.8% and 19.0% prevalence of Megrivirus B and 32.2% and 39.7% prevalence of Pigeon picornavirus B in the group of pigeons exhibiting signs of enteropathy and in the group of asymptomatic pigeons, respectively. In turn, digital droplet PCR showed a considerably higher number of genome copies of both viruses in sick than in asymptomatic pigeons. The results of quantitative analysis leave the role of picornaviruses in enteropathies of pigeons unclear.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%