Rapid detection of rifampicin in fish using immunochromatographic strips

Abstract

In this study we developed a rapid and simple assay to detect RFP in fish using an immunochromatographic assay. We produced a sensitive and selective monoclonal antibody against RFP with a half-maximal inhibitory concentration (IC 50 ) of 10.7 ng/mL. Fish samples were extracted with ethyl acetate, then centrifuged at 8000 rpm for 10 min, and concentrated by drying over nitrogen. We found under optimal conditions, that the RFP cut-off limits for the test strip was 10 µg/mL in both 0.01 M phosphate buffered saline and fish samples, and each test could be completed within 5 min. Therefore, immunochromatographic test can be used as a reliable, rapid, and sensitive method for the detection of RFP in fish.

Rapid and sensitive detection of chlordimeform in cucumber and tomato samples using an immunochromatographic assay

“…The purified mAbs were used to prepare colloidal gold-labeled anti-CDM mAbs. 35 Firstly, 10 mL colloidal gold solution was mixed with 160 μL K 2 CO 3 solution and 250 μL mAbs (0.2 mg mL −1 ). After 50 minutes, we added 500 μL of 10% BSA to the solution and incubated for 2 h at room temperature.…”

The Application of Different Technologies for Removal of Rifampicin From Aquatic Environments: A Recent Review

“…RIF is relatively nontoxic [18]. However, a high dose of RIF can be toxic to biological systems and cause various side effects such as allergic reactions, nausea, diarrhea, vomiting, hepatotoxicity, loss of appetite, immunological disturbances, oxidative conjunctivitis, fatigue, headache and organic brain syndrome [19,20]. Once in the body, RIF is partly digested and the its excess is excreted by humans through urine and faeces to the sewage systems, and was detected in effluents from wastewater treatment plants since the conventional treatment plants cannot effectively remove this type of resistant compounds [21].…”

“…It produces residues in the edible tissues of fish products and hereby can be harmful for human health. It can also lead to drug resistance in the human body [19]. Studies on RIF residues in the aquaculture environment have shown that RIF with a high risk of developing resistance leads to ARGs in fish pathogens, increases antibiotic resistance, alters the microbial community, and potentially transfers ARGs to terrestrial animal and human pathogens [26,27].…”