Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers

Li, Jie; Jing, Le; Song, Yuzhu; Zhang, Jinyang; Chen, Qiang; Wang, Binghui; Xia, Xueshan; Han, Qinqin

doi:10.1186/s11671-018-2709-9

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…To achieve a selective detection, one simply needs to optimize an appropriate signaling antibody that will pair with GAGs. The need for one antibody instead of two (as required for constructing traditional sandwich-type LFSA sensors) will significantly shorten the screening time 47,48 when applied toward a new pathogen or variant, as well as drastically lowering the cost, potentially 10-fold, as compared to current LFSA technologies. 98,99 GlycoGrip has remarkable promise as a widespread tool for capturing and detecting current and emerging viruses.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in the case of LFSA sandwich-type detection, pairs of antibodies or aptamers must be screened for both capturing and reporting, which delays sensor design and development. 47 , 48 In contrast, GAGs could serve as universal capture agents for various viral analytes including mutant strains, resulting in an easily generalizable LF platform. This could greatly reduce the assay’s antibody screening and optimization time, which typically takes anywhere from 12 to 16 weeks.…”

Section: Introductionmentioning

confidence: 99%

“…Using traditional bioreceptors, such as antibodies and aptamers as immobilizing agents in LFSA design, requires these receptors to be screened and optimized, as well as necessitating that LF strips be reconfigured, for every desired viral analyte and potential mutant strains. Furthermore, in the case of LFSA sandwich-type detection, pairs of antibodies or aptamers must be screened for both capturing and reporting, which delays sensor design and development. , In contrast, GAGs could serve as universal capture agents for various viral analytes including mutant strains, resulting in an easily generalizable LF platform. This could greatly reduce the assay’s antibody screening and optimization time, which typically takes anywhere from 12 to 16 weeks. , This could enable fast adaptation of GAG-based LF strips for current and emerging viruses and provide cheap and simple ways to administer viral antigen tests for critically undertested communities during times of global health crises.…”

Section: Introductionmentioning

confidence: 99%

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GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses

et al. 2021

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Inspired by the role of cell-surface glycoproteins as coreceptors for pathogens, we report the development of GlycoGrip : a glycopolymer-based lateral flow assay for detecting SARS-CoV-2 and its variants. GlycoGrip utilizes glycopolymers for primary capture and antispike antibodies labeled with gold nanoparticles for signal-generating detection. A lock-step integration between experiment and computation has enabled efficient optimization of GlycoGrip test strips which can selectively, sensitively, and rapidly detect SARS-CoV-2 and its variants in biofluids. Employing the power of the glycocalyx in a diagnostic assay has distinct advantages over conventional immunoassays as glycopolymers can bind to antigens in a multivalent capacity and are highly adaptable for mutated strains. As new variants of SARS-CoV-2 are identified, GlycoGrip will serve as a highly reconfigurable biosensor for their detection. Additionally, via extensive ensemble-based docking simulations which incorporate protein and glycan motion, we have elucidated important clues as to how heparan sulfate and other glycocalyx components may bind the spike glycoprotein during SARS-CoV-2 host-cell infection. GlycoGrip is a promising and generalizable alternative to costly, labor-intensive RT-PCR, and we envision it will be broadly useful, including for rural or low-income populations that are historically undertested and under-reported in infection statistics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses

et al. 2021

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“…Not only this, rongalite is an attractive building block to assemble sultine derivatives which are useful masked dienes partners for Diels‐Alder (DA) reaction to construct intricate molecules which are otherwise challenging or almost impossible by other means . On the other hand, very recently Han and co‐workers have revealed a promising role of aptamers for the detection of rongalite in food with high sensitivity and specificity through sandwich‐type lateral flow strip and enzyme‐linked aptamer assays . Therefore, keeping the above stated potential applications of this valuable reagent in mind, herein I have tried to disclose its applicability not only in modern synthetic organic chemistry but also its utility in material sciences.…”

Section: Introductionmentioning

confidence: 99%

New Dimensions in Rongalite Chemistry: The Land of Opportunities in Organic Synthesis and Material Sciences

Ali

2020

ChemistrySelect

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Rongalite, first time reported in the chemical structure in 1905, also known as bruggolite or sodium formaldehyde sulfoxylate, having good solubility and stability in organic media, is not only a cheap and commercially available (in kg amount) reagent with a range of industrial applications for instance emulsion polymerization, an excellent decolouring agent, antioxidant in pharmaceutical development, and an antidote for heavy metals, but also used as an effective regents in a plethora of organic reactions particularly in redox reactions as a powerful reducing agent. More interestingly, in recent years, it has also shown its impact in material sciences for the preparation of CdTe, CoTe2, and Ag2Se thin films, nanowire networks, hollow nanospheres, copper nanoparticles (Cu‐Nps), CdTe‐quantum dots and so forth. Keeping in mind the importance of this vital reagent in both organic chemistry as well as in material sciences, herein I intended to report the recent developments along with a brief overview of old literature wherever necessary to showcase the utility of this valuable reagent. This particular review article not only covers the applications of this environmentaly benign reagent in organic synthesis but also its usefulness in material sciences in particular nanosciences has been exposed for the first time.

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“…Recently, several direct methods that adopted rongalite itself as the detection maker were reported as alternative methods for the rapid detection of rongalite. For instance, based on a rongalite-specific aptamer, Li et al established a sandwich lateral flow strip (LFIA) assay with the limit of detection (LOD) of 1.0 μg/mL [ 15 ]; Jing et al developed a sandwich-type enzyme-linked immunosorbent assay (ELISA) with the LOD of 0.57 ng/mL ( Table S1 ) [ 16 ]. Although the aptamer-participated methods were rapid and sensitive, the key reagent aptamer with high specificity and affinity was hard to be prepared.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Fluorescence Sensor for the Direct Quantification of Rongalite in Foodstuffs

Chen

Huang

et al. 2022

Foods

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Rongalite was reported illegally used as a food additive for bleaching purposes and improving the tenderness of foodstuffs, which may endanger public health. At present, rongalite was mostly detected by indirect methods via derivatization or determining its decomposition products. In this study, we developed a new fluorescence sensor for the direct quantification of rongalite based on the principles: (1) dopamine reacts with resorcinol and generates strong fluorophore (azamonardine); (2) rongalite could inhibit the production of fluorophores and then result in lower fluorescence intensity. Hence, the rongalite concentration was inversely proportional to fluorescence intensity of fluorophore. Several crucial reaction conditions of fluorescence sensor were further optimized, such as dopamine and resorcinol concentration, pH values, and reaction time. Under the optimal conditions, the limit of detection of fluorescence sensor was 0.28–0.38 μg/g in vermicelli, wheat and rice powder samples, exhibiting almost 3.5-fold improvement compared to that of lateral flow immunoassay. Moreover, the detection time was substantially decreased to 20 min. The recoveries in spiked samples were 80.7–102.1% with a coefficient of variation of less than 12.6%. In summary, we developed a direct, high throughput, selective and accurate fluorescence sensor that poses a promising application for the rapid detection of rongalite in foodstuffs.

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Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers

Cited by 15 publications

References 23 publications

GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses

GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses

New Dimensions in Rongalite Chemistry: The Land of Opportunities in Organic Synthesis and Material Sciences

A Rapid Fluorescence Sensor for the Direct Quantification of Rongalite in Foodstuffs

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