Rapid detection of S. pyogenes and S. pneumoniae in pleural fluid for diagnosis of parapneumonic empyema

De Schuyter, Kelly; Boelens, Jerina; Messiaen, Anne-Sophie; Schelstraete, Petra; Verhasselt, Bruno; Huis In’t Veld, Diana; Callens, Steven; Sermijn, Erica; Vande Weygaerde, Yannick; Vandendriesche, Stien

doi:10.1007/s10096-023-04710-w

Cited by 3 publications

References 28 publications

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Group A Streptococcal meningitis in children: a short case series and systematic review

Dou,

Li,

et al. 2024

Eur J Clin Microbiol Infect Dis

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Background Group A streptococcal(GAS) meningitis is a severe disease with a high case fatality rate. In the era of increasing GAS meningitis, our understanding about this disease is limited. Purpose To gain a better understanding about GAS meningitis. Methods Five new cases with GAS meningitis were reported. GAS meningitis related literatures were searched for systematic review in PUBMED and EMBASE. Case reports and case series on paediatric cases were included. Information on demographics, risk factors, symptoms, treatments, outcomes, and emm types of GAS was summarized. Results Totally 263 cases were included. Among 100 individuals, 9.9% (8/81) had prior varicella, 11.1% (9/81) had anatomical factors, and 53.2% (42/79) had extracranial infections. Soft tissue infections were common among infants (10/29, 34.5%), while ear/sinus infections were more prevalent in children ≥ 3 years (21/42, 50.0%). The overall case fatality rate (CFR) was 16.2% (12/74). High risk of death was found in patients with shock or systemic complications, young children(< 3 years) and cases related to hematogenic spread. The predominate cause of death was shock(6/8). Among the 163 patients included in case series studies, ear/sinus infections ranged from 21.4 to 62.5%, while STSS/shock ranged from 12.5 to 35.7%, and the CFR ranged from 5.9 to 42.9%. Conclusions A history of varicella, soft tissue infections, parameningeal infections and CSF leaks are important clinical clues to GAS in children with meningitis. Young children and hematogenic spread related cases need to be closely monitored for shock due to the high risk of death.

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Group A Streptococcal meningitis in children: a short case series and systematic review

Dou,

Li,

et al. 2024

Eur J Clin Microbiol Infect Dis

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Diagnostic Accuracy of a Rapid Antigen Detection Test for Group A Streptococcus in Children With Pleural Empyema

Faury,

Flamant,

Ferroni

et al. 2024

Pediatric Infectious Disease Journal

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Integration of Group A Streptococcus Rapid Tests with the Open Fluidic CandyCollect Device

Sanchez,

Robertson,

Shinkawa

et al. 2024

Preprint

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The CandyCollect device is a lollipop-inspired open fluidic oral sampling device designed to provide a comfortable user sampling experience. We demonstrate that the CandyCollect device can be coupled with a rapid antigen detection test (RADT) kit designed for Group A Streptococcus (GAS). Throughin vitroexperiments with pooled saliva spiked withStreptococcus pyogeneswe tested various reagents and elution volumes to optimize the RADT readout from CandyCollect device samples. The resulting optimized protocol uses the kit-provided reagents and lateral flow assay (LFA) while replacing the kit’s pharyngeal swab with the CandyCollect device, reducing the elution solution volume, and substituting the tube used for elution to accommodate the CandyCollect device. Positive test results were detected by eye with bacterial concentrations as low as the manufacturer’s “minimal detection limit” - 1.5×105CFU/mL. LFA strips were also scanned and quantified with image analysis software to determine the signal-to-baseline ratio (SBR) and categorize positive test results without human bias. We tested our optimized protocol for integrating CandyCollect and RADT using CandyCollect clinical samples from pediatric patients (n=6) who were previously diagnosed with GAS pharyngitis via pharyngeal swabs tested with RADT as part of their clinical care. The LFA results of these CandyCollect devices and interspersed negative controls were determined by independent observers, with positive results obtained in four of the six participants on at least one LFA replicate. Taken together, our results show that CandyCollect devices from children with GAS pharyngitis can be tested using LFA rapid tests.Table of Contents/Abstract Figure

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Rapid detection of S. pyogenes and S. pneumoniae in pleural fluid for diagnosis of parapneumonic empyema

Cited by 3 publications

References 28 publications

Group A Streptococcal meningitis in children: a short case series and systematic review

Group A Streptococcal meningitis in children: a short case series and systematic review

Diagnostic Accuracy of a Rapid Antigen Detection Test for Group A Streptococcus in Children With Pleural Empyema

Integration of Group A Streptococcus Rapid Tests with the Open Fluidic CandyCollect Device

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