Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip

Kong, Dezhao; Liu, Liqiang; Song, Shanshan; Zheng, Qinfen; Wu, Xiaoling; Kuang, Hua

doi:10.1080/09540105.2017.1337085

Cited by 17 publications

(14 citation statements)

References 25 publications

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“…The sensitivity of the ICT strip largely depended on the AuNPs-labeled mAb and the coating antigen immobilized on the conjugate pad and NC membrane, respectively [ 37 ]. Therefore, two types of mAb (2A2 and 3C6) and coating antigens (PMB-GA-OVA1 and PMB-GA-OVA2) were compared using 0 ng/mL and 250 ng/mL of the PMB standard solution.…”

Section: Resultsmentioning

confidence: 99%

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A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues

Liu

Song

et al. 2018

Nanomaterials

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Increasing attention is now being directed to the utilization of polymyxin B (PMB) as a last-line treatment for life-threatening infections caused by multidrug resistant Gram-negative bacteria. Unfortunately, polymyxins resistance is also increasingly reported, leaving a serious threat to human health. Therefore, the establishment of rapid detection methods for PMB residues is highly essential to ensure public health. In this study, two monoclonal antibodies (mAb; 2A2 and 3C6) were obtained using PMB-bovine serum albumin as the immunogen and PMB-ovalbumin as the coating antigen, which were prepared with N-(γ-maleimidobutyryloxy) succinimide ester and glutaraldehyde as cross-linking agents, respectively. Through an indirect competitive enzyme-linked immunosorbent assay, resultant two mAbs were compared and the results indicated that 3C6 showed higher sensitivity with a half maximum inhibition concentration of 13.13 ng/mL. Based on 3C6, a gold nanoparticles (AuNPs)-based immunochromatographic test (ICT) strip was then established, the mechanism of which is that free PMB competes with the fixed coating antigen to combine with mAb labeled by AuNPs. Using ICT strip to detect milk and animal feed samples revealed the visible detection limits were 25 ng/mL and 500 μg/kg, respectively and the cutoff limits were 100 ng/mL and 1000 μg/kg, respectively. The ICT strip provides results within 15 min, facilitating rapid and semi-quantitative analysis of PMB residues in milk and animal feed.

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Section: Resultsmentioning

confidence: 99%

“…Running buffer has an influence on the flow rate of the AuNPs-labeled mAb and therefore, the intensity of the control line [ 37 , 38 ]. As a consequence, different running buffers were prepared and compared by detecting PMB-negative and PMB-positive samples.…”

Section: Resultsmentioning

confidence: 99%

A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues

Liu

Song

et al. 2018

Nanomaterials

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“…The mouse with the highest inhibitory rate and titre was selected for cell fusion. The selected hybridoma cell lines were cultured and intraperitoneally injected into mice for ascites production (Kong, Liu, Song, Zheng, et al, 2017). After purification with octanoic acid-ammonium sulfate precipitation (Liang et al, 2014;Wang, Zheng, et al, 2017) and dialysis, monoclonal antibodies against BPS were obtained.…”

Section: Production Of the Bps Monoclonal Antibody (Mab)mentioning

confidence: 99%

IC-ELISA and immunochromatographic strip assay based monoclonal antibody for the rapid detection of bisphenol S

Lü

Luo

et al. 2019

Food and Agricultural Immunology

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A highly sensitive and specific monoclonal antibody against bisphenol S (BPS) was prepared. The derived BPS was coupled to keyhole limpet hemocyanin as the immunogen and ovalbumin as the coating antigen. Based on monoclonal antibodies, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established, and the conditions of action were optimized. The half maximal inhibitory concentration (IC 50 ) of BPS was 0.228 ng/mL and the linear range was 0.064-0.810 ng/mL. In the recovery test for the milk samples, the recovery rates were in the range of 89%-95% and coefficient of variation ranged from 1.0% to 6.0% respectively. In addition, the cut-off value of the immunochromatographic strip detection method in milk samples was 5 ng/mL. Therefore, both methods are suitable for use in milk samples. Furthermore, this immunochromatographic strip detection method is suitable for on-site testing and screening of very large samples.

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“…Although these methods are very accurate and sensitive, they are costly and require complex sample pretreatment steps and trained personnel (Mukunzi, Suryoprabowo, Song, Liu, & Kuang, 2018;Qu et al, 2019;Wang et al, 2018;Zeng, Jiang, Liu, Song, & Kuang, 2018). Immunoassays are superior to chromatographic methods in screening tests for their simplicity, sensitivity, and high-throughput capabilities (Chen et al, 2017; Kong et al, 2017;Wang, Luo, Chen, Huang, & Jiang, 2016). Recently, some immunoassays for detecting residues of flunixin and other NSAIDs have been reported (Lin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

Chen

Peng

Liu

et al. 2019

Food and Agricultural Immunology

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Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk, Food and Agricultural Immunology, 30:1, 320-332, ABSTRACTThe flunixin residues in foods of animal origin could pose hazards for human health. In this paper, a broad-selectivity polyclonal antibody was obtained using 5-hydroxyflunixin coupled with bovine serum albumin as the immunogen. The semi-inhibitory concentration (IC 50 ) of the polyclonal antibody was 1.43 ng/mL for flunixin and 0.29 ng/mL for 5-hydroxyflunixin, respectively, which were far below the maximum residue limits set by the United States, the European Union and China. The limits of detection were calculated to be 2.98 μg/kg for flunixin in bovine muscle and 0.78 μg/L for 5-hydroxyflunixin in milk. In spike and recovery tests, the average recovery ranged from 83% to 105%, with a satisfying coefficient of variance ranging from 5.8% to 11.3%. Furthermore, the ELISA method was validated by a well-established LC-MS/MS method. These results proved that the established method is suitable for flunixin and 5-hydroxyflunixin residue analysis. ARTICLE HISTORY

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Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip

Cited by 17 publications

References 25 publications

A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues

A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues

IC-ELISA and immunochromatographic strip assay based monoclonal antibody for the rapid detection of bisphenol S

Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

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