Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

Subbotin, Sergei A.

doi:10.3390/ijms25020844

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…The new species can be distinguished from A. angusticaudatus [39] by the presence of males vs. absence; a longer body length of females, 728.0 (564.0-978.0) µm vs. (550-610) µm; a lower b value of females, 9.0 (8.0-10.0) vs. (11-13); a higher c value of females 17.0 (15.0-21.0) vs. (12)(13)(14)(15)(16)(17)(18); and a lower c ′ value of females, 3.4 (2.8-4.0) vs. 5.1. From A. cyrtus, by having an almost straight body habitus of females vs. arc-shaped; swelling type stylet knobs vs. distinctly knobbed; spicule shape (condylus elongated rounded, rostrum small, blunt conical, lamina less wide, gradually tapers towards the rounded distal end vs. (after drawings) condylus and rostrum appear pointed, lamina is wider, distal end appears pointed); the position of the tail mucro (central vs. appears ventrally located); habitat (post-harvest potato field vs. lichens, mushroom amended with flax and straw additives); longer body lengths of females, 728.0 (564.0-978.0) µm vs. (500-570) µm, and males, 720.0 (624.0-859.0) µm vs. (500-510) µm; a higher a value of females, 34.0 (31.0-38.0) vs. (24-28), and males, 33.5 (30.0-36.5) vs. (26)(27).…”

Section: Description Of Aphelenchoides Prairiensis N Spmentioning

confidence: 99%

“…was achieved by integrative taxonomical approaches that combined morphological, morphometrical, and molecular data. These methods constitute the basis for the development of posterior species-specific molecular probes, for example, the diagnostic recombinase polymerase amplification (RPA) assays and lateral flow dipsticks (LF), developed by Subbotin [ 12 ] for the separation of the strawberry foliar nematode Aphelenchoides fragariae from other important foliar pathogens, such as the summer crimp nematode of strawberry Aphelenchoides besseyi or the rice white tip nematode Aphelenchoides oryzae [ 12 ]. Control measures that have been successfully used to manage infestations of aphelenchid species, particularly Aphelenchoides spp., on key crops have been recently reviewed by de Souza et al [ 13 ] and included the application of chemical nematicides, cultural practices, crop rotation with nonhost plants, biological control agents, induced resistance (silicon or endophytic microorganisms), and blocking parasitism genes (i.e., using RNA interference (RNAi) or genome editing (typically CRISPR/Cas) to disrupt the targets of the effectors in the plant).…”

Section: Introductionmentioning

confidence: 99%

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Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada

Munawar,

Castillo,

Yevtushenko

2024

Microorganisms

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The nematode family Aphelenchoididiae is considered fungal-feeding, predatory, or root hair feeders. Some members of this family are universally present in agricultural landscapes and are an integral part of soil health and conservation studies. In the present soil nematode biodiversity survey, we detected four species of the genera Aphelenchus, Aphelenchoides, and Robustodorus. Because fungal-feeding nematodes from southern Alberta have not previously been reported, we conducted a detailed morphological and molecular investigation, identifying these species as Aphelenchus avenae, Aphelenchoides limberi, Aphelenchoides prairiensis n. sp. and Robustodorus paramegadorus n. sp. The first two species we document as new records from southern Alberta, whereas A. prairiensis n. sp. and R. paramegadorus n. sp. we describe in detail as new taxa. Briefly, A. prairiensis n. sp. is an amphimictic species having 4 lateral lines; hemispherical anteriorly flattened lip region; delicate stylet and swelling-like stylet knobs; excretory pore at the posterior edge of nerve ring. Female tail conical, gradually tapering towards a truncated end with single mucro. Spicule 23.0 (20.0–25.0) µm long having elongated rounded condylus, small, blunt conical rostrum, and lamina that gradually tapers towards the rounded distal end; three pairs of caudal papillae were present on the male tail. Robustodorus paramegadorus n. sp., is a parthenogenetic species with 3 lines in the lateral fields; lip region rounded, anteriorly flattened; stylet robust, with knobs rounded to bean-shaped; excretory pore located posterior to nerve ring; reproductive components were quite indiscernible with a short 24.0 (18.0–27.0) µm post-vulval uterine sac; tail conical, ending with pointed to wedge-shaped tip. We performed molecular characterizations for each species and constructed phylogenetic trees to study the phylogenetic relationship of these aphelenchid species. The discovery of A. prairiensis n. sp. and R. paramegadorus n. sp. indicates that soil nematode diversity is relatively unexplored in southern Alberta. The findings of this study will significantly enhance the identification processes and may contribute towards future soil health and biodiversity efforts.

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Section: Description Of Aphelenchoides Prairiensis N Spmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada

Munawar,

Castillo,

Yevtushenko

2024

Microorganisms

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Molecular and Morphological Characteristics of a Novel Cyst Nematode in the Rhizosphere of Artemisia lavandulaefolia DC. in Gansu Province, Northwest China

Guo,

Li,

Wei

et al. 2024

Pathogens

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Cyst nematodes are obligate parasitic nematodes found in the fields of many cultivated crops. These nematodes, which have great economic importance, pose a threat to food security, though they are frequently ignored or misdiagnosed as pests because of covert parasitism. A cyst nematode population parasitizing on Artemisia lavandulaefolia DC., one of the traditional Chinese medicines was collected in Gansu Province. The species was diagnosed using integrative taxonomy and molecular approaches. The cyst population is spherical or lemon-shaped, light brown or dark brown in color, with a long neck and a protruding vulval cone. The stylet of the second-stage juvenile is strong, and the front end of the ball at the base of the stylet is concave; the median bulb and excretory pore are prominent; the tail is blunt and circular, and the transparent tail is usually shorter than the stylet. A phylogenetic analysis was carried out using the internal transcribed spacer (ITS) and 28S genes of ribosomal DNA, which further confirmed the presence of Cactodera chenopodiae. According to our literature review, this is the first report on C. chenopodiae in Compositae. By following this research, we can better understand the challenges posed by A. lavandulaefolia DC. and develop effective strategies for managing its spread and impacts. This will help to protect vulnerable ecosystems and ensure the sustainability of agricultural and forestry activities in affected areas.

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Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

Cited by 2 publications

References 33 publications

Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada

Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada

Molecular and Morphological Characteristics of a Novel Cyst Nematode in the Rhizosphere of Artemisia lavandulaefolia DC. in Gansu Province, Northwest China

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