Rapid detection of trace Salmonella in milk and chicken by immunomagnetic separation in combination with a chemiluminescence microparticle immunoassay

Li, Jingwen; Liu, Qingjun; Yue, Wan; Wu, Xiaosheng; Yang, Yin; Zhao, Ruixue; Chen, Erning; Cheng, Xiaoyan; Du, Min

doi:10.1007/s00216-019-01991-z

Cited by 45 publications

(25 citation statements)

References 48 publications

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“…This also explains the results reported by Li, Liu et al. (2019), who observed that the CE enhanced as the particle size (180 nm, 300 nm, 600 nm, 1 µm, and 2.8 µm) increased in the chicken culture filtrate and milk. For now, the most common and successful IMPs are obtained based on Dynabeads (Dynal, Oslo, Norway), which are polystyrene‐based microbeads with particle sizes ranging from 1 to 10 µm.…”

Section: The Main Factors To Improve Ims Performancesupporting

confidence: 89%

“…It has been found that 96% of Salmonella cells (10 4 CFU/mL) in buffers could be recovered by IMPs with the diameter of 100 nm, while that for the 500 and 1,000 nm particles were 89 and 61%, respectively; in chicken rinse, the recoveries of Salmonella cells were 78, 44, and 21% with the changes of particles sizes from 100 to 1,000 nm (Chen & Park, 2018). Li, Liu, Wan, Wu, and Du (2019) also evaluated the effects of particle size (180nm, 300 nm, 600 nm, 1 µm, and 2.8 µm) on the IMS of Salmonella in PBST buffer. The results showed that the CE value was always greater than 75% and decreased with the increasing of particle size.…”

Section: The Main Factors To Improve Ims Performancementioning

confidence: 99%

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Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment techniques is very crucial for the detection of food microorganisms. With the sig

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Section: The Main Factors To Improve Ims Performancesupporting

confidence: 89%

Section: The Main Factors To Improve Ims Performancementioning

confidence: 99%

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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“…It could be argued that this approach has not eliminated the initial enrichment process as it adds significant time to the overall procedure but using the current imaging analysis approach was not possible without compromising the detection limit. Several other rapid detection methods based on PCR, phage and immunoassay still use enrichment as a pre-detection step to enhance the performance of the methods [14,[33][34][35][36][37]. There is another acceptable reason to consider enrichment.…”

Section: Plos Onementioning

confidence: 99%

Rapid detection of Escherichia coli using bacteriophage-induced lysis and image analysis

Wisuthiphaet

Young

et al. 2020

PLoS ONE

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Rapid detection of bacterial pathogens is a critical unmet need for both food and environmental samples such as irrigation water. As a part of the Food safety Modernization Act (FSMA), The Produce Safety rule has established several requirements for testing for the presence of generic Escherichia coli in water, but the current method available for testing (EPA M1603) demands specified multiple colony verification and highly trained personnel to perform these tests. The purpose of the study was to assess a phage induced bacterial lysis using quantitative image analysis to achieve rapid detection of E. coli at low concentrations within 8 hours. This study aimed to develop a simple yet highly sensitive and specific approach to detect target bacteria in complex matrices. In the study, E. coli cells were first enriched in tryptic soy broth (TSB), followed by T7 phage induced lysis, concentration, staining and fluorescent imaging. Image analysis was conducted including image pre-processing, image segmentation and quantitatively analysis of cellular morphological features (area, eccentricity and full width at half maximum). Challenge experiments using realistic matrices, including simulated fresh produce wash water, coconut water and spinach wash water, demonstrated the method can be applied for use in situations that occur in food processing facilities. The results indicated E. coli cells that are lysed by T7 phages demonstrated significantly (P < 0.05) higher extracellular DNA release, altered cellular shape (from rod to circular) and diffused fluorescent signal intensity. Using this biosensing strategy, a sensitivity to detect Escherichia coli at 10 CFU/ml within 8 hours was achieved, both in laboratory medium and in complex matrices. The proposed phage based biosensing strategy enables rapid detection of bacteria and is applicable to analysis of food systems. Furthermore, the steps involved in this assay can be automated to enable detection of target bacteria in food facilities without extensive resources.

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“…In contrast, no fluorescence signal was observed when the same number of bacteria was spiked into the cabbage sample, indicating that the cabbage sample required an additional pretreatment step, including longer enrichment (Figure 5a). Despite its limitations, this automated IMS device is speculated to be effective in rapidly detecting pathogenic bacteria [33]. Table 2.…”

Section: Initial Concentrationmentioning

confidence: 99%

Detection of E. coli O157:H7 in Food Using Automated Immunomagnetic Separation Combined with Real-Time PCR

et al. 2020

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In this study, we describe the development of an automated immunomagnetic separation device combined with real-time polymerase chain reaction (PCR) for detecting foodborne bacteria. Immunomagnetic separation (IMS) is a well-known method for the separation and concentration of target bacteria from a large volume of food samples. Magnetic beads functionalized with an antibody provide selectivity for target bacteria such as Escherichia coli O157:H7. Moreover, compared to conventional methods, real-time PCR enables high-sensitivity detection of target bacteria. The method proposed in this study involves three steps: (1) pre-enrichment, (2) automated IMS and concentration of target bacteria, and (3) detection of target bacteria by real-time PCR. Using food samples with a working sample volume as large as 250 mL, the whole process only requires 3 h. As a result, target bacteria in the range of 101–102 colony-forming units per mg or g of sample can be detected in food samples, such as milk, ground beef, and cabbage, by using the proposed approach. We anticipate that the automated IMS system combined with real-time PCR will contribute to the development of a fully automated system for detecting foodborne bacteria and serve as a multi-tester for a variety of bacterial strains in the capacity of a sample-to-answer device in the near future.

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Rapid detection of trace Salmonella in milk and chicken by immunomagnetic separation in combination with a chemiluminescence microparticle immunoassay

Cited by 45 publications

References 48 publications

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Rapid detection of Escherichia coli using bacteriophage-induced lysis and image analysis

Detection of E. coli O157:H7 in Food Using Automated Immunomagnetic Separation Combined with Real-Time PCR

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