2017
DOI: 10.1016/j.diagmicrobio.2017.06.012
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Rapid detection of Trypanosoma cruzi by colorimetric loop-mediated isothermal amplification (LAMP): A potential novel tool for the detection of congenital Chagas infection

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Cited by 41 publications
(34 citation statements)
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“…Therefore, this prototype kit detects T.cruzi DNA at concentrations 1 x 10 −2 fg/µL using purified DNA and 1x10 −2 parasite equivalents/mL ("parasite equivalent" means the equivalent genomic content of one parasite cell) in extracts from spiked EDTA blood samples. The analytical sensitivity of this LAMP assay was superior to other studies using a LAMP procedure based on the 18S rDNA gene [107,109].…”
Section: Lamp In Diagnosis Of American Try-panosomiasis (T Cruzi)mentioning
confidence: 72%
“…Therefore, this prototype kit detects T.cruzi DNA at concentrations 1 x 10 −2 fg/µL using purified DNA and 1x10 −2 parasite equivalents/mL ("parasite equivalent" means the equivalent genomic content of one parasite cell) in extracts from spiked EDTA blood samples. The analytical sensitivity of this LAMP assay was superior to other studies using a LAMP procedure based on the 18S rDNA gene [107,109].…”
Section: Lamp In Diagnosis Of American Try-panosomiasis (T Cruzi)mentioning
confidence: 72%
“… 44 Other groups have developed similar LAMP kits to detect T. cruzi DNA. 45 Another platform that meets the criteria for implementation of molecular diagnosis at such sites is recombinase polymerase amplification (RPA), which also enables sensitive, specific and rapid amplification of DNA under isothermal conditions. The RPA reaction runs optimally at 37°C–42°C which may present an advantage in terms of energy costs saving.…”
Section: Implementation Of Molecular Tools To Test For Congenital Chamentioning
confidence: 99%
“…However, multiple infant specimens are required to maximize sensitivity [18]. A number of PCR assays are now available to detect congenital T. cruzi infections, including quantitative PCR [40] and a recent loop-mediated isothermal amplification (LAMP) protocol [51]. Molecular techniques require considerable equipment, expertise and rigorous quality control, which are difficult to implement and sustain in resource-limited settings.…”
Section: Congenital Chagas Disease Screening and Diagnosismentioning
confidence: 99%