Rapid Determination of Creatinine in Human Urine by Microchip Electrophoresis with LED Induced Fluorescence Detection

Wang, Shuping; Li, Xinchun; Yang, Jianping; Yang, Xiupei; Hou, Fenghua; Chen, Zuanguang

doi:10.1007/s10337-012-2324-3

Cited by 24 publications

(8 citation statements)

References 31 publications

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“…9,10 Based on NIR absorption spectrum of urine samples and multivariate regression model Cr prediction accuracy of 9 mg/dL was reported by Shaw et al 6 The estimation the amount of Cr in the spent dialysate using UV-absorbance approach has been reported which does not need blood samples and disposables of chemicals and is fast. 12 Rapid determination of Cr in human urine-based°uorescence detection for evaluating renal function was documented from the study of Wang et al 13 Surface enhanced Raman scattering (SERS) has been also proposed by few groups for the detection of Cr. 11,23 Nevertheless, as far as we are aware, our observed clinical Cr blood concentration near patient's bedside is lower than those in the reported literatures which primarily measure Cr in urine or serum samples.…”

Section: Resultsmentioning

confidence: 99%

“…5 The importance of accurate Cr monitoring as a biomarker to aid diagnosis of renal functioning of both healthy individuals and dialysis patients is widely recognized. Beyond the standard hospital techniques, simple, portable and low-cost methods of Cr determination using optical approaches have been proposed by several research groups, among them Near-infrared (NIR) spectroscopy, 6,7 Raman spectroscopy, [8][9][10] surface-enhanced Raman scattering, 11 UV-absorbance, 12°u orescence, 13 etc. In this regard, NIR spectroscopy o®ers a promising technological platform for Cr monitoring.…”

Section: Introductionmentioning

confidence: 99%

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Determination of creatinine level in patient blood samples by Fourier NIR spectroscopy and multivariate analysis in comparison with biochemical assay

Barnea

Abookasis

2019

J. Innov. Opt. Health Sci.

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In this paper, in vivo spectra from 23 patients’ blood samples with various Creatinine (Cr) concentration levels ranging from 0.96 to 12.5 mg/dL were measured using Fourier transform near-infrared spectrometer (FT-NIRS) and spectrum quantitative analysis method. Since Cr undergoes passive filtration, it serves as a key biomarker of kidneys function via the estimation of glomerular filtration rate. Thus, increased blood Cr concentration reflects impaired renal function. After spectra pre-processing and outlier exclusion, a spectral model was developed based on partial least squares regression (PLSR) method, wherein Cr concentrations correlated with filtered NIR spectra across several peaks, where Cr is known to absorb NIR light. Several statistical metrics were applied to estimate the model efficiency during data analysis. Comparison of spectra-derived concentrations to reference Cr measurements by the current gold-standard Jaffe’s method held in hospital lab revealed a Cr prediction accuracy of 1.64[Formula: see text]mg/dL with good correlation of [Formula: see text]. Bland-Altman plots were used to compare between our calculations and reference lab values and reveal minimal bias between the two. The finding presented the potential of FT-NIRS coupled with PLSR technique for Cr determination.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of creatinine level in patient blood samples by Fourier NIR spectroscopy and multivariate analysis in comparison with biochemical assay

Barnea

Abookasis

2019

J. Innov. Opt. Health Sci.

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“…The approaches to study the function of liver [139], kidney [140][141][142][143], lacrimal gland [144,145], and thyroid gland [146] included miniaturised immunoassays, gel electrophoresis, and zone electrophoresis.…”

Section: Organ Diseases and Dysfunctionsmentioning

confidence: 99%

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Wuethrich

Quirino

2019

Analytica Chimica Acta

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A core element in clinical diagnostics is the data interpretation obtained through the analysis of patient samples. To obtain relevant and reliable information, a methodological approach of sample preparation, separation, and detection is required. Traditionally, these steps are performed independently and stepwise. Microchip capillary electrophoresis (MCE) can provide rapid and high-resolution separation with the capability to integrate a streamlined and complete diagnostic workflow suitable for the point-of-care setting. Whilst standard clinical diagnostics methods normally require hours to days to retrieve specific patient data, MCE can reduce the time to minutes, hastening the delivery of treatment options for the patients. This review covers the advances in MCE for disease detection from 2008 to 2017. Miniaturised diagnostic approaches that required an electrophoretic separation step prior to the detection of the biological samples are reviewed. In the two main sections, the discussion is focused on the technical setup used to suit MCE for disease detection and on the strategies that have been applied to study various diseases. Throughout these discussions MCE is compared to other techniques to create context of the potential and challenges of MCE. A comprehensive table categorised based on the studied disease using MCE is provided. We also comment on future challenges that remain to be addressed.

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“…In this context, Wang and Chatrathi reported a microfabricated electrophoresis chip for bioassay of renal markers , and García and Henry a microchip CE with pulsed electrochemical detection for the direct detection of renal function . More recently, Yang et al used a CE microchip with LED induced fluorescence detection for a rapid determination of creatinine in human urine . In this article, we report on the use of the Medimate Multireader® as POC disposable device to detect creatinine levels above 100 μM in human serum.…”

Section: Introductionmentioning

confidence: 95%

Point of care creatinine measurement for diagnosis of renal disease using a disposable microchip

et al. 2013

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A point-of-care device for the determination of elevated creatinine levels in blood is reported. This device potentially offers a new and simple clinical regime for the determination of creatinine that will give huge time savings and removal of several steps of determination. The test employs a disposable prefilled microchip and the handheld Medimate Multireader®. By optimizing the analytical conditions it was found that the LOD of the proposed method was 87 μM creatinine, close to the normal human serum levels that are in the range of 60 to 100 μM. A statistical analysis of the residual shows a normal distribution, indicating the absence of systematic errors in the proposed method. The test can be used to distinguish patients with renal insufficiency (creatinine levels >100 μM) from healthy persons. Long-term monitoring could furthermore distinguish between acute renal failure and chronic kidney disease by the rate of creatinine concentration rise.

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Rapid Determination of Creatinine in Human Urine by Microchip Electrophoresis with LED Induced Fluorescence Detection

Cited by 24 publications

References 31 publications

Determination of creatinine level in patient blood samples by Fourier NIR spectroscopy and multivariate analysis in comparison with biochemical assay

Determination of creatinine level in patient blood samples by Fourier NIR spectroscopy and multivariate analysis in comparison with biochemical assay

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Point of care creatinine measurement for diagnosis of renal disease using a disposable microchip

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