Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium‐sensitized fluorescence spectrophotometry

Guo, Changchuan; Dong, Peiwu; Chu, Zhijie; Wang, Lei; Jiang, Wei

doi:10.1002/bio.1008

Cited by 27 publications

(9 citation statements)

References 20 publications

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“…Thus, a rapid, low cost, and accessible procedure for the quantification of the FQs in dosage forms is desirable. Various techniques have been utilized for their analysis including UV-vis spectrophotometry [13][14][15][16][17] and spectrofluorometry [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical and Spectroscopic Studies on the Interaction of Gatifloxacin, Moxifloxacin and Sparfloxacin with DNA and Their Analytical Applications

et al. 2010

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The electrochemical oxidation of the three fluoroquinolone drugs FQs: gatifloxacin GTF, moxifloxacin MXF and sparfloxacin SPF, at the bare and DNA-modified glassy carbon electrodes has been studied by voltammetric techniques. The three FQs showed one irreversible oxidation peak at potential range 0.85-0.91 V vs. Ag-AgCl, in phosphate buffer of pH 7.0. Differential pulse voltammetry (DPV) and UV-absorption spectroscopic techniques were employed to probe the interaction between the FQs and calf thymus double stranded deoxyribonucleic acid (ds CT-DNA). From electrochemical data, the binding constant between DNA and the gatifloxacin, moxifloxacin and sparfloxacin are calculated to be 3228, 2596 and 2857 M À1 respectively. Based on electrochemical and spectroscopic results, the mode of binding of fluoroquinolone to DNA through combined effect of intercalation and electrostatic interaction was concluded. A detection scheme based on a preconcentration and differential pulse voltammetric (DPV) determination at dsDNA modified glassy carbon electrode (DNA/GCE) was proposed for the trace determination of the studied analytes. The developed method was successfully applied to the determination of the FQs in pharmaceutical formulations.

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Section: Introductionmentioning

confidence: 99%

Electrochemical and Spectroscopic Studies on the Interaction of Gatifloxacin, Moxifloxacin and Sparfloxacin with DNA and Their Analytical Applications

et al. 2010

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“…Several methods have been reported for the determination of GTFX in the samples of human urine=serum (Guo et al 2008;Ocana, Barragan, and Callejon 2005;Lucia et al 2005;Nguyen et al 2004), rabbit serum (Yu and Liu 2011), human blood (El-Desoky 2009), and human plasma (Al Dgither, Alvi, and Hammami 2006;Liang, Kays, and Sowinski 2002), pharmaceutical dosage forms (Gouda, El-Sheikh, and Amin 2008), pure and dosage form (Jane, Subrahmanyam, and Sathyanarayana 2006), tablets (Salgado and Oliveira 2005), injection (Zhu, Gong, and Yu 2008), eye drops (Shirke and Pai 2004), and food (aquatic products; Zhao et al 2011), Most methods were developed based on reaction of GTFX with various reagents to form colored derivatives for spectrophotometric detection. In recent years, high-performance liquid chromatography (HPLC) methods have been reported for the determination of GTFX in various samples (Al Al Dgither, Alvi, and Hammami 2006;Liang, Kays, and Sowinski 2002;Shirke and Pai 2004;Zhao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Determination of Gatifloxacin in Milk and Biological Fluids by Inhibitory Resonance Fluorescence Spectrometry

Jing-hui

Hui-xian

et al. 2013

Analytical Letters

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A novel method for the determination of trace gatifloxacin was established by resonance fluorescence techniques. It was based on the fact that oxidation of rhodamin 6G by hydrogen peroxide and Cu 2þ was inhibited by gatifloxacin in Tris-HCl buffer medium and in presence of cetyl trimethyl ammonium bromide. The enhanced intensity (DF) of resonance fluorescence at 540 nm was proportional to the concentration of gatifloxacin in the range of 9.8 Â 10 À3 À 1.75 lg mL À1 . The limit of detection was 2.9 ng mL À1 and the average recovery was 101.8% (n ¼ 6) for gatifloxacin. The present method was applied to the determination of trace gatifloxacin in milk and biological fluids, and the obtained results were in good agreement with those obtained by HPLC.

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“…Quinolones are one of the most widely used groups of antibacterial drugs in the medical field due to their wide antimicrobial spectrum, strong sterilization force and safety, therefore it is important to study quantitative and qualitative differences in their structure–activity relationships . Quinolones are prepared by chemical synthesis, and any problems are overcome by transformation in the drug structure.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence spectra, fluorescence quantum yield and dissociation constant of sarafloxacin

Liu

Duan

et al. 2016

Luminescence

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In this study, the fluorescence spectra of sarafloxacin (SAR) under different pH conditions were investigated to determine the structural changes due to protonation that result from change in pH. At pH < 1.02, SAR exists in the H L form for which the maximum fluorescence emission wavelength was about 455 nm. At pH 1.87-4.94, SAR exists in the H L form in which H L loses one proton in the nitrogen molecule at the 1-position in the quinoline ring. Fluorescence intensity was strong and steady and the maximum emission wavelength was 458 nm. At pH 7.14-9.30, the maximum emission wavelengths were gradually blue shifted to 430 nm with increase in pH, here SAR exists in the form of a bipolar ion HL in which H L loses a carboxyl group proton. At pH > 11.6, HL transforms into anionic L in which HL loses one proton from the piperazine ring, leading to a decrease in fluorescence intensity, and the maximum emission wavelength was red shifted to approximately 466 nm. The two-step dissociation constant pKa for SAR was calculated, pK was 6.06 ± 0.37 and pK for SAR was 10.53 ± 0.19. In a pH 3.62 buffer solution with quinine sulfate as the reference, the fluorescence quantum yield of SAR at the maximum excitation wavelength of 276 nm was 0.09.

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Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium‐sensitized fluorescence spectrophotometry

Cited by 27 publications

References 20 publications

Electrochemical and Spectroscopic Studies on the Interaction of Gatifloxacin, Moxifloxacin and Sparfloxacin with DNA and Their Analytical Applications

Electrochemical and Spectroscopic Studies on the Interaction of Gatifloxacin, Moxifloxacin and Sparfloxacin with DNA and Their Analytical Applications

Determination of Gatifloxacin in Milk and Biological Fluids by Inhibitory Resonance Fluorescence Spectrometry

Fluorescence spectra, fluorescence quantum yield and dissociation constant of sarafloxacin

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