Rapid determination of <i>Alternaria</i> mycotoxins in tomato samples by pressurised liquid extraction coupled to liquid chromatography with fluorescence detection

Rico-Yuste, Alberto; Gómez-Arribas, Lidia N.; Pérez-Conde, M.C.; Urraca, Javier L.; Moreno‐Bondi, María C.

doi:10.1080/19440049.2018.1512759

Cited by 14 publications

(9 citation statements)

References 20 publications

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“…The LOD and LOQ values of the recent (HPLC or LC-MS/MS) methods are typically in the range of 0.01-6.0 μg/kg and 0.03-12.0 μg/kg, respectively [6]. Our Mg 2+ -enhanced methods proved to be more sensitive than the HPLC-FLD method reported by Rico-Yuste et al (LOD = 7.0 μg/kg, LOQ = 20.0 μg/kg) [24]. In addition, the sensitivity of the Mg 2+ -enhanced C8 method is only slightly lower compared to certain LC-MS/MS methods previously reported regarding the measurement of AOH from wheat (LOD = 0.75 μg/kg, LOQ = 2.5 μg/kg) [25] and tomato-based products (LOD = 1.4 μg/kg, LOQ = 3.5 μg/kg) [26].…”

Section: Probing the Analytical Application Of Aoh-mg 2+ Interaction To Increase The Sensitivity Of Hplc-fld Methodsmentioning

confidence: 55%

“…(LOD = 7.0 µg/kg, LOQ = 20.0 µg/kg) [24]. In addition, the sensitivity of the Mg 2+enhanced C8 method is only slightly lower compared to certain LC-MS/MS methods previously reported regarding the measurement of AOH from wheat (LOD = 0.75 µg/kg, LOQ = 2.5 µg/kg) [25] and tomato-based products (LOD = 1.4 µg/kg, LOQ = 3.5 µg/kg) [26].…”

Section: Probing the Analytical Application Of Aoh-mg 2+ Interaction To Increase The Sensitivity Of Hplc-fld Methodsmentioning

confidence: 99%

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Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin

Fliszár-Nyúl

Lemli

Kunsági‐Máté

et al. 2021

IJMS

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Alternariol (AOH) is an emerging mycotoxin produced by Alternaria molds. It occurs as a contaminant e.g., in oilseeds, cereals, grapes, and tomatoes. Chronic exposure to AOH may cause genotoxic and endocrine disruptor effects. Our recent studies demonstrated that the fluorescence signal of AOH can be strongly affected by the environmental pH as well as by the presence of serum albumin or cyclodextrins. In the current study, we aimed to characterize the most optimal circumstances regarding the highly sensitive fluorescent detection of AOH. Therefore, the further detailed investigation of the microenvironment on the fluorescence signal of the mycotoxin has been performed, including the effects of different buffers, organic solvents, detergents, and cations. Organic solvents (acetonitrile and methanol) caused only slight increase in the emission signal of AOH, while detergents (sodium dodecyl sulfate and Triton-X100) and Ca2+ induced considerably higher enhancement in the fluorescence of the mycotoxin. In addition, Mg2+ proved to be a superior fluorescence enhancer of the AOH. Spectroscopic and modeling studies suggest the formation of low-affinity AOH-Mg2+ complexes. The effect of Mg2+ was also tested in two HPLC assays: Our results show that Mg2+ can considerably increase the fluorescence signal of AOH even in a chromatographic system.

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Section: Probing the Analytical Application Of Aoh-mg 2+ Interaction To Increase The Sensitivity Of Hplc-fld Methodsmentioning

confidence: 55%

Section: Probing the Analytical Application Of Aoh-mg 2+ Interaction To Increase The Sensitivity Of Hplc-fld Methodsmentioning

confidence: 99%

Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin

Fliszár-Nyúl

Lemli

Kunsági‐Máté

et al. 2021

IJMS

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“…It has been confirmed that this method can be used to extract various mycotoxins from cereals [ 48 ]. Pressurized Liquid Extraction (PLE), also known as accelerated solvent extraction (ASE), is the same method as SLE but performed under increased pressure and temperature in a pressure-resistant vessel [ 49 , 50 ]. In these methods, conventional solvents at high temperatures (100–180 °C) and pressures (1500–2000 psi) are used to improve the extraction of analytes from the matrix [ 51 ].…”

Section: Food Sampling and Sample Preparationmentioning

confidence: 99%

“…In these methods, conventional solvents at high temperatures (100–180 °C) and pressures (1500–2000 psi) are used to improve the extraction of analytes from the matrix [ 51 ]. PLE has been used to detect mycotoxins produced by Alternaria alternata in a tomato sample [ 50 ]. The next method is the Supercritical Fluid Extraction (SFE).…”

Section: Food Sampling and Sample Preparationmentioning

confidence: 99%

The Existing Methods and Novel Approaches in Mycotoxins’ Detection

et al. 2021

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Mycotoxins represent a wide range of secondary, naturally occurring and practically unavoidable fungal metabolites. They contaminate various agricultural commodities like cereals, maize, peanuts, fruits, and feed at any stage in pre- or post-harvest conditions. Consumption of mycotoxin-contaminated food and feed can cause acute or chronic toxicity in human and animals. The risk that is posed to public health have prompted the need to develop methods of analysis and detection of mycotoxins in food products. Mycotoxins wide range of structural diversity, high chemical stability, and low concentrations in tested samples require robust, effective, and comprehensible detection methods. This review summarizes current methods, such as chromatographic and immunochemical techniques, as well as novel, alternative approaches like biosensors, electronic noses, or molecularly imprinted polymers that have been successfully applied in detection and identification of various mycotoxins in food commodities. In order to highlight the significance of sampling and sample treatment in the analytical process, these steps have been comprehensively described.

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“…The use of ultraviolet (UV) and diode array (DAD) detection coupled to HPLC did not account for more than 14%, jointly, of the applications for the analysis of cereals and 28% for the analysis of fruits and vegetables [20]. The use of liquid chromatography with fluorescence detection has been used in selected cases for the analysis of AOH, AME and ALT in cereals [7] and tomato products [24].…”

Section: Introductionmentioning

confidence: 99%

Determination of Alternaria Toxins in Food by SPE and LC-IDMS: Development and In-House Validation of a Candidate Method for Standardisation

Gonçalves

Tölgyesi²,

Bouten

et al. 2022

Separations

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Alternaria toxins (ATs) are frequently found contaminants in foodstuffs (e.g., alternariol), often reaching high concentrations (e.g., tenuazonic acid). They can spoil a wide variety of food categories (e.g., cereals, vegetables, seeds and drinks) and storage at fridge temperatures does not prevent the growth of Alternaria fungi. Therefore, reliable and validated analytical methods are needed to protect human health and to ensure a transparent and fair trade. This paper describes new technical features that improved a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of ATs in tomato, wheat and sunflower seeds. This analytical method should be simple to implement in different laboratories across the EU and thus be an attractive candidate for standardisation. The major element for improvement was the use of isotopically labelled internal standards, only recently commercially available, thereby reducing the sample handling and improving the accuracy of the results. In addition, the sample extraction and the solid-phase extraction (SPE) enrichment/clean-up were fine-tuned, whereas a more suitable analytical column (XSelect HSS T3) with improved selectivity was also employed. Overall, this method shows adequate precision (repeatability < 5.7% RSD; intermediate precision < 7.0% RSD) and trueness (recoveries ranging from 74% to 112%). The limits of quantification in wheat (the most analytically demanding matrix) vary between 0.19 and 1.40 µg/kg. These figures were deemed satisfactory by the European Committee for Standardization (CEN) and have formed the basis for a subsequent interlaboratory validation study. The corresponding standard was published by CEN in 2021.

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Rapid determination of Alternaria mycotoxins in tomato samples by pressurised liquid extraction coupled to liquid chromatography with fluorescence detection

Cited by 14 publications

References 20 publications

Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin

Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin

The Existing Methods and Novel Approaches in Mycotoxins’ Detection

Determination of Alternaria Toxins in Food by SPE and LC-IDMS: Development and In-House Validation of a Candidate Method for Standardisation

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