Rapid determination of letrozole, citalopram and their metabolites by high performance liquid chromatography-fluorescence detection in urine: Method validation and application to real samples

Rodríguez, José Manuel Fernández; Castañeda, Gregorio; Muñoz, Lorena

doi:10.1016/j.jchromb.2012.11.015

Cited by 22 publications

(11 citation statements)

References 34 publications

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“…This work established that under similar chromatographic conditions fluorescence detection was six times more sensitive than UV detection. Rodriguez et al [9] reported the simultaneous analysis of letrozole, citalopram and their metabolites. Letrozole is a selective nonsteroidal inhibitor of the aromatase system that is used for the treatment of erstrogen dependent breast cancers, while citalopram is an antidepressant.…”

Section: Mental Disordersmentioning

confidence: 99%

Quantitative Analysis of Drugs in Biological Matrices by HPLC Hyphenated to Fluorescence Detection

Lipka

Vaccher

2015

Bioanalysis

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An overview of the state-of-the art in HPLC coupled with fluorescence detection is presented. Over the last 20 years, the increasing number of methodological papers on this topic (4082 between 1994 and 2004 and 7725 between 2004 and 2014) is testament to its utility in bioanalytical applications. Compared with conventional UV absorbance detection used in HPLC, fluorescence detection can greatly enhance the sensitivity leading to limits of detection similar to those obtained with mass spectrometry, offering researchers a sensitive, robust and relatively inexpensive instrumental method. This work will focus on the analysis of pharmaceutical compounds in different biological matrices, either naturally fluorescent or derivatized with a fluorescent agent, and some of them chiral. Therapeutic applications, sample preparation and derivatization, sensitivity for each example are described.

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Section: Mental Disordersmentioning

confidence: 99%

Quantitative Analysis of Drugs in Biological Matrices by HPLC Hyphenated to Fluorescence Detection

Lipka

Vaccher

2015

Bioanalysis

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“…HPLC is one of the most efficient and robust separation techniques due to its convenience, simple operation, strong separation ability and wide sample application. HPLC with Ultraviolet-Visible (UV-VIS) [1], fluorescence [2], diode array (DAD) [3] mass spectrometry (LC-MS) [4] or tandem mass spectrometry (LC-MS/MS) [3,[5][6][7][8][9][10] detection methods were often applied for analysis of different psychotropic drugs in various biological samples such as: serum [5,6], plasma [4], urine [2], saliva [3], breast milk [7]. Most often HPLC analysis of psychotropic drugs was performed on octadecyl (C18) or octyl (C8) columns with mobile phases containing a mixture of organic modifier, water with addition of formic or acetic acid [5,11,12], acidic buffer [1,6,8], basic buffer [4], ammonium salts (acetate or formate) [13,14] or amine [15].…”

Section: Introductionmentioning

confidence: 99%

Optimization of chromatographic systems for analysis of selected psychotropic drugs and their metabolites in serum and saliva by HPLC in order to monitor therapeutic drugs

et al. 2019

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Retention, separation selectivity and system efficiency of selected basic psychotropic drugs (clozapine, aripiprazole, vortioxetine and zolpidem) and drug metabolites (desmethylclozapine, clozapine N-oxide and dehydroaripiprazole) on Hydro RP, Phenyl-Hexyl and Polar RP columns were studied. Mobile phases containing methanol or acetonitrile as organic modifiers, acetate buffer at pH 3.5 and addition of diethylamine (DEA) as a silanol blocker were applied. Significant differences in the retention, peak shapes and systems' efficiency of the investigated compounds were obtained depending on the tested chemically bonded stationary phases with various ligands. Based on the obtained results the Phenyl-Hexyl column was selected for analysis of the drugs and their metabolites in human serum and saliva samples.Solid phase extraction (SPE) was applied for sample pretreatment. The best SPE-HPLC-DAD procedure was used for simultaneous analysis of clozapine, aripiprazole and their metabolites in body fluids. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was applied for confirmation of the presence of the investigated compounds in biological samples. The lower limit of quantification (LLOQ) of clozapine obtained using the proposed method was 10 ng/mL. The validated method for determining the presence of clozapine and its main metabolite was successfully applied in therapeutic drug monitoring.

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“…It is a steroidal aromatase inhibitor usually used as oral tablets during a hormone therapy to reduce the free estrogen derivatives . Letrozole (LTZ) (4,4′‐[1 H ‐1,2,4‐triazol‐1‐yl‐methylene]bisbenzonitrile is a nonsteroidal aromatase inhibitor used during the treatment of an estrogen‐dependent breast cancer . Paclitaxel (PCX) ((2α,4α,5β,7β,10β,13α)‐4,10‐bis(acetyloxy)‐13‐{[(2 R ,3 S )‐3‐(benzoylamino)‐2‐hydroxy‐3‐phenylpropanoyl]oxy}‐1,7‐dihydroxy‐9‐oxo‐5,20‐epoxytax‐11‐en‐2‐yl benzoate), a natural product extracted from specific yaw trees on the Pacific Coast, is another anticancer pharmaceutical that has been widely used in chemotherapy treatments such as lung, breast, and ovarian cancers.…”

Section: Introductionmentioning

confidence: 99%

Extraction and determination of trace amounts of three anticancer pharmaceuticals in urine by three‐phase hollow fiber liquid‐phase microextraction based on two immiscible organic solvents followed by high‐performance liquid chromatography

Nazaripour

Yamini

Bagheri

2018

J of Separation Science

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An automated three-phase hollow fiber liquid-phase microextraction based on two immiscible organic solvents followed by high-performance liquid chromatography with UV-Vis detection method was applied for the extraction and determination of exemestane, letrozole, and paclitaxel in water and urine samples. n-Dodecane was selected as the supported liquid membrane and its polarity was justified by trioctylphosphine oxide. Acetonitrile was used as an organic acceptor phase with desirable immiscibility having n-dodecane. All the effective parameters of the microextraction procedure such as type of the organic acceptor phase, the supported liquid membrane composition, extraction time, pH of the donor phase, hollow fiber length, stirring rate, and ionic strength were evaluated and optimized separately by a one variable at-a-time method. Under the optimal conditions, the linear dynamic ranges were 1.8-200 (R = 0.9991), 0.9-200 (R = 0.9987) and 1.2-200 μg/L (R = 0.9983), and the limits of detection were 0.6, 0.3, and 0.4 μg/L for exemestane, letrozole, and paclitaxel, respectively. To evaluate the capability of the proposed method in the analysis of biological samples, three different urinary samples were analyzed under the optimal conditions. The relative recoveries of the three pharmaceuticals were in the range of 91-107.3% for these three analytes.

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Rapid determination of letrozole, citalopram and their metabolites by high performance liquid chromatography-fluorescence detection in urine: Method validation and application to real samples

Cited by 22 publications

References 34 publications

Quantitative Analysis of Drugs in Biological Matrices by HPLC Hyphenated to Fluorescence Detection

Quantitative Analysis of Drugs in Biological Matrices by HPLC Hyphenated to Fluorescence Detection

Optimization of chromatographic systems for analysis of selected psychotropic drugs and their metabolites in serum and saliva by HPLC in order to monitor therapeutic drugs

Extraction and determination of trace amounts of three anticancer pharmaceuticals in urine by three‐phase hollow fiber liquid‐phase microextraction based on two immiscible organic solvents followed by high‐performance liquid chromatography

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