Rapid determination of molecular parameters of synthetic polymers by precipitation/redissolution high-performance liquid chromatography using ?molded? monolithic column

o, Miroslav Jan; Skora, David; Švec, František; Frchet, Jean M. J.; Schweer, Johannes; Holm, Ragnar

doi:10.1002/1099-0518(20000801)38:15<2767::aid-pola170>3.0.co;2-b

Cited by 40 publications

(16 citation statements)

References 30 publications

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“…Each data point represents the mean of 15 successive injections shown below and its confidence interval at a risk of 5%. Evaluated separations: [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Batch-to-batch variation of retention times for cytochrome c using 20 cm × 100 μm I.D. monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) capillary column prepared using thermally (A, B, and C for batches 1-3, respectively) and photochemically (D, E, and F for batches 1-3, respectively) initiated polymerization.…”

Section: Discussionmentioning

confidence: 99%

“…Since their invention in the early 1990s, rigid porous polymer-based monolithic columns have shown their unique suitability for the rapid separations of large molecules such as proteins [1][2][3][4][5], nucleic acids [6][7][8], and synthetic polymers [9][10][11]. The original analytical scale size columns were supplemented with their capillary counterparts in the early 2000s [5,[12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Stability and repeatability of capillary columns based on porous monoliths of poly(butyl methacrylate-co-ethylene dimethacrylate)

Geiser

Eeltink

Švec

et al. 2007

Journal of Chromatography A

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Monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) capillary columns have been prepared via either thermally or photochemically initiated polymerization of the corresponding monomers and the repeatability of their preparation has been explored. Three separate batches of five columns each were prepared using thermal and photochemical initiation for a total of thirty columns. All thirty capillary columns were tested in liquid chromatography-electrospray ionizationmass spectrometry mode for the separation of a model mixture of three proteins -ribonuclease A, cytochrome c and myoglobin. Excellent repeatability of retention times was observed for the proteins as evidenced by relative standard deviation (RSD) values of less than 1.5%. Somewhat broader variations with RSD values of up to 10% were observed for the pressure drop in the columns. The stability of retention times was also monitored using a single monolithic column and no significant shifts in either retention times or back pressure was observed in a series of almost 2200 consecutive protein separations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stability and repeatability of capillary columns based on porous monoliths of poly(butyl methacrylate-co-ethylene dimethacrylate)

Geiser

Eeltink

Švec

et al. 2007

Journal of Chromatography A

Self Cite

110

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“…The monolithic devices are covalently attached to walls of the microchannel and their adequate mechanical strength and low flow resistance allow their use even in systems requiring very high flow rates. We have demonstrated in our previous research that this monolithic material is very stable and does not change its properties even after several months of continuous operation [25]. Although monolithic mixers consisting of only one monomer-cross-linker (HEMA-EDMA) combination have been demonstrated, the chemistry of the monolithic device can easily be changed by choosing different monomers.…”

Section: Discussionmentioning

confidence: 99%

Porous polymer monoliths: Simple and efficient mixers prepared by direct polymerization in the channels of microfluidic chips

et al. 2001

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Porous monolithic polymers have been prepared by photoinitiated polymerization of mixtures consisting of 2-hydroxyethyl methacrylate, ethylene dimethacrylate, UV-sensitive free radical initiator and porogenic solvent within channels of specifically designed microfluidic chips and used as micromixers. Substituting azobisisobutyronitrile with 2,2-dimethoxy-2-phenylacetophenone considerably accelerated the kinetics of the polymerization. Mixtures of cyclohexanol and 1 -dodecanol and of hexane and methanol were used, respectively, to control the porous properties and therefore the mixing efficiency of the device. The performance of the monolithic mixers has been tested by pumping aqueous solutions of two fluorescent dyes at various flow rates and monitoring the point at which the boundary of both streams completely disappears. Best results were achieved with a monolithic mixer containing very large irregular pores.

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“…However, it is difficult to achieve both high flow rates and high resolution with columns packed with microparticles as they often exhibit prohibitively high flow resistance or slow mass transfer [24]. We also found that application of stepwise gradients enabled much better control of the elution and better resolution was achieved [29]. We have already demonstrated that separation media consisting of "molded" porous monoliths can be used for the rapid separations of styrene oligomers and various polymers according to their molecular weights [24, 27 -29].…”

Section: Introductionmentioning

confidence: 87%

Effect of porosity and surface chemistry on the characterization of synthetic polymers by HPLC using porous polymer monolithic columns

et al. 2002

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Monolithic 5064.6 mm ID poly(styrene-co-divinylbenzene), poly(glycidyl methacrylate-co-ethylene dimethacrylate), and poly(2,3-dihydroxypropyl methacrylate-co-ethylene dimethacrylate) columns have been tested in the high-performance liquid chromatography (HPLC) separation of polystyrenes and poly(methyl methacrylate) standards. The separation process involves precipitation of the macromolecules on the macroporous monolithic column followed by progressive redissolution and elution utilizing a gradient of the mobile phase. While very good separations of individual components of mixtures of both polystyrenes and poly(methyl methacrylates) can be readily achieved using poly(styrene-co-divinylbenzene) monolith, methacrylate-based columns do not afford good separations under identical conditions. This unexpected finding appears to be the result of significant differences between the porous properties and chemistries of the two types of monolithic materials.

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Rapid determination of molecular parameters of synthetic polymers by precipitation/redissolution high-performance liquid chromatography using ?molded? monolithic column

Cited by 40 publications

References 30 publications

Stability and repeatability of capillary columns based on porous monoliths of poly(butyl methacrylate-co-ethylene dimethacrylate)

Stability and repeatability of capillary columns based on porous monoliths of poly(butyl methacrylate-co-ethylene dimethacrylate)

Porous polymer monoliths: Simple and efficient mixers prepared by direct polymerization in the channels of microfluidic chips

Effect of porosity and surface chemistry on the characterization of synthetic polymers by HPLC using porous polymer monolithic columns

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