Rapid determination of puerarin by near-infrared spectroscopy during percolation and concentration process of puerariae lobatae radix

Abstract

Background:Gegen (Puerariae Labatae Radix) is one of the important medicines in Traditional Chinese Medicine. The studies showed that Gegen and its preparation had effective actions for atherosclerosis.Objective:Near-infrared (NIR) was used to develop a method for rapid determination of puerarin during percolation and concentration process of Gegen.Materials and Methods:About ten batches of samples were collected with high-performance liquid chromatography analysis values as reference, calibration models are generated by partial least-squares (PLS) regression as linear regression, and artificial neural networks (ANN) as nonlinear regression.Results:The root mean square error of prediction for the PLS and ANN model was 0.0396 and 0.0365 and correlation coefficients (r2) was 97.79% and 98.47%, respectively.Conclusions:The NIR model for the rapid analysis of puerarin can be used for on-line quality control in the percolation and concentration process.SUMMARY Near-infrared was used to develop a method for on-line quality control in the percolation and concentration process of GegenCalibration models are generated by partial least-squares (PLS) regression as linear regression and artificial neural networks (ANN) as non-linear regressionThe root mean square error of prediction for the PLS and ANN model was 0.0396 and 0.0365 and correlation coefficients (r2) was 97.79% and 98.47%, respectively. Abbreviations used: NIR: Near-Infrared Spectroscopy; Gegen: Puerariae Loabatae Radix; TCM: Traditional Chinese Medicine; PLS: Partial least-squares; ANN: Artificial neural networks; RMSEP: Root mean square error of validation; R2: Correlation coefficients; PAT: Process analytical technology; FDA: The Food and Drug Administration; Rcal: Calibration set; RMSECV: Root mean square errors of cross-validation; RPD: Residual predictive deviation; SLS: Straight Line Subtraction; MLP: Multi-Layer Perceptron; MSE: Mean square error.

Comparison of different extraction methods of active ingredients of Chinese medicine and natural products

“…At present, the classical extraction methods of CM and natural products mainly include decoction [25, 26], maceration [27–29], reflux [30, 31], percolation [21], steam distillation [32] and sublimation [33, 34]. With the development of modern separation science and equipment, some new technologies such as ultrasonic wave‐assisted extraction (UAE) [35–38], microwave‐assisted extraction (MAE) [39–42], supercritical fluid extraction (SFE) [43–47], enzyme‐assisted extraction (EAE) [48, 23, 49], membrane separation extraction (MSE) [50–57], ultrafine grinding technology (UGT) [58–64], microporous resin adsorption technology (MRAT) [65–70], semi‐bionic extraction (SBE) [71, 72], immune selective extraction (ISE) [73–77], molecular‐distillation technology [78–82], molecularly‐imprinted polymer extraction (MIPE) [83–88], UPE [89–93], liquid chromatographic extraction (LCE) [94–99], solid‐phase extraction (SPE) [100–107], liquid–liquid extraction (LLE) [108–112], etc.…”

“…In the operation, the extract of medicine will be heated and distilled, and the volatile solvents will be distilled and condensed, and flow back to the leaching device, and so on until the effective ingredients are completely extracted. It can be divided into atmospheric pressure [30] and reduced pressure reflux process [31]. Soxhlet device is the most commonly used one.…”

“…At present, the classical extraction methods of CM and natural products mainly include decoction [25,26], maceration [27][28][29], reflux [30,31], percolation [21], steam distillation [32] and sublimation [33,34]. With the development of modern separation science and equipment, some new technologies such as ultrasonic waveassisted extraction (UAE) [35][36][37][38], microwave-assisted extraction (MAE) [39][40][41][42], supercritical fluid extraction (SFE) [43][44][45][46][47], enzyme-assisted extraction (EAE) [48,23,49], membrane separation extraction (MSE) [50][51][52][53][54][55][56][57], ultrafine grinding technology (UGT) [58][59][60][61][62][63][64], microporous resin adsorption technology (MRAT) [65][66][67][68]…”

Rapid Quality Assessment of Polygoni Multiflori Radix Based on Near-Infrared Spectroscopy

“…Tis provides valuable technical support for the comprehensive development of Coptidis rhizome plant resources [17]. It can also be used in conjunction with chemometric methods to rapidly determine the puerarin content during the percolation and concentration process of Puerariae Lobatae Radix, with RMSE P of 0.0396 mg/ml and 0.0365 mg/ml and R 2 of 97.79% and 98.47%, respectively [18]. Te NIRS technique can be used for the rapid determination of two chemical indicators, 5-HMF and 420 nm absorbance values, with the RMSE P less than 0.03 and the R 2 greater than 0.99 in the TCM process.…”