Rapid Determination of Quinolone Resistance in
            <i>Acinetobacter</i>
            spp

Hujer, Kristine M.; Hujer, Andrea M.; Endimiani, Andrea; Thomson, Jodi M.; Adams, Mark D.; Goglin, Karrie; Rather, Philip N.; Pennella, Thuy Trang D.; Massire, Christian; Eshoo, Mark W.; Sampath, Rangarajan; Blyn, Lawrence B.; Ecker, David J.; Bonomo, Robert A.

doi:10.1128/jcm.02380-08

Cited by 69 publications

(42 citation statements)

References 29 publications

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“…Many A. baumannii isolates manifest high levels of resistance (36) to antibiotics such as ampicillin-sulbactam (6), carbapenems (28,29), aminoglycosides (10,26), tetracyclines (15,17), and quinolones (8,16). Of particular concern is the emergence of A. baumannii strains resistant to all commonly used antibiotics, including colistin (13,30), polymyxin B (27), or tigecycline (25).…”

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confidence: 99%

Evaluation of the Trimeric Autotransporter Ata as a Vaccine Candidate against Acinetobacter baumannii Infections

et al. 2012

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Acinetobacter baumannii is a multidrug-resistant (MDR) nosocomial pathogen for which immunotherapeutic alternatives are needed. We previously identified a surface autotransporter of A. baumannii, Ata, that bound to various extracellular matrix/basal membrane proteins and was required for full virulence, biofilm formation, and the adhesion of A. baumannii to collagen type IV. We show here that Ata binding to collagen type IV was inhibited by antibodies to Ata. In addition, in the presence of complement and polymorphonuclear cells (PMNs), antibodies to Ata were highly opsonic against A. baumannii ATCC 17978 and showed low to moderate killing activity against four heterologous A. baumannii strains, whereas in the absence of PMNs, antibody to Ata efficiently promoted complement-dependent bactericidal killing of all of the tested A. baumannii isolates. Using a pneumonia model of infection in both immunocompetent and immunocompromised mice, we found that, compared to normal rabbit sera, antisera to Ata significantly reduced the levels of A. baumannii ATCC 17978 and two MDR strains in the lungs of infected mice. The ability of Ata to engender anti-adhesive, bactericidal, opsonophagocytic, and protective antibodies validates its potential use as an antigenic target against MDR A. baumannii infections.

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Evaluation of the Trimeric Autotransporter Ata as a Vaccine Candidate against Acinetobacter baumannii Infections

et al. 2012

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“…Using a hydrolysis reaction to identify ␤-lactamases and carbapenemases, MALDI-TOF is able to accurately report the presence of carbapenemases. PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) quickly identifies Gram-negative bacteria and can target identification of common resistance genes such as gyrA, parC, and bla KPC with Ͼ96% sensitivity and specificity (4,6). Both of these applications are awaiting large-scale trials in the clinical arena.…”

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Detection of Extended-Spectrum β-Lactamase and Klebsiella pneumoniae Carbapenemase Genes Directly from Blood Cultures by Use of a Nucleic Acid Microarray

et al. 2012

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The growing crisis of multidrug-resistant (MDR) Gram-negative bacteria requires that current technologies permit the rapid detection of extended-spectrum ␤-lactamase (bla ESBL ) and Klebsiella pneumoniae carbapenemase (bla KPC ) genes. In the present study, we assessed the performance characteristics of a commercially available nucleic acid microarray system for the detection of bla ESBL and bla KPC genes directly from positive blood cultures. Using blood cultures (BCs) that contained Gram-negative bacilli identified by Gram staining, we isolated bacterial DNA using spin columns (BC-C) and rapid water lysis (BC-W). Twenty ESBL/KPC-positive and 20 ESBL/KPC-negative blood culture samples, as well as 20 non-lactose-fermenting organisms, were tested. The 20 isolates that were ESBL positive by phenotypic testing were also evaluated on solid medium (SM), and the DNA was extracted by use of a spin column (SM-C). The resulting 140 DNA extractions were assessed for DNA quantity and quality using 260/280-nm absorbance ratios, and DNA microarray analysis was performed in a blinded fashion. Microarray and phenotypic results were concordant for 98.3% of BC-W, 90% of BC-C, and 95% of SM-C samples. Compared to phenotypic testing, the sensitivity and specificity for BC-C samples were 88.9% and 100%, respectively, and for BC-W samples, the sensitivity and specificity were 94.4% and 100%, respectively. BC-W samples yielded the highest concordance with phenotypic results. Nucleic acid microarrays offer promise in the identification of bla ESBL and bla KPC genes directly from blood cultures, thereby reducing the time to identification of these important pathogens.

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“…lates was established as the simultaneous mutations resulting in changes at codon 83 for gyrA and at codon 80 for parC (10,20,21).…”

Section: Resultsmentioning

confidence: 99%

Fast Assessment of Resistance to Carbapenems and Ciprofloxacin of Clinical Strains of Acinetobacter baumannii

et al. 2012

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dInfections caused by multidrug-resistant Acinetobacter baumannii constitute a major life-threatening problem worldwide, and early adequate antibiotic therapy is decisive for success. For these reasons, rapid detection of antibiotic susceptibility in this pathogen is a clinical challenge. Two variants of the Micromax kit were evaluated for a rapid detection in situ of susceptibility or resistance to meropenem or ciprofloxacin, separately, in 322 clinical isolates. Release of the nucleoid is the criterion of susceptibility to the beta-lactams (carbapenems), whereas diffusion of DNA fragments emerging from the nucleoid characterizes the quinolone activity. All the susceptible and resistant strains were correctly categorized in 100 min according to the MIC results and CLSI criteria. Thus, our technology is a promising tool for rapid identification of carbapenem and quinolone resistance of A. baumannii strains in hospital settings.

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Rapid Determination of Quinolone Resistance in Acinetobacter spp

Cited by 69 publications

References 29 publications

Evaluation of the Trimeric Autotransporter Ata as a Vaccine Candidate against Acinetobacter baumannii Infections

Evaluation of the Trimeric Autotransporter Ata as a Vaccine Candidate against Acinetobacter baumannii Infections

Detection of Extended-Spectrum β-Lactamase and Klebsiella pneumoniae Carbapenemase Genes Directly from Blood Cultures by Use of a Nucleic Acid Microarray

Fast Assessment of Resistance to Carbapenems and Ciprofloxacin of Clinical Strains of Acinetobacter baumannii

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