Rapid determination of trace levels of tetracyclines in surface water using a continuous flow manifold coupled to a capillary electrophoresis system

Nozal, Leonor; Arce, Lourdes; Simonet, B.M.; Ríos, Ángel; Valcárcel, Miguel

doi:10.1016/j.aca.2004.04.050

Cited by 76 publications

(44 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Figure 7 shows the electropherogram corresponding to a water sample spiked with a 5 ng/mL concentration of each tetracycline derivative. This CE method enabled the detection up to 2 ng/mL of tetracyclines in water samples [21]. …”

Section: Antibiotics In Environmental Samples: Their Role As Emergingmentioning

confidence: 99%

“…In this sense, offline sample treatment by SPE, LLE, and SLE has been performed. However, the online coupling of sample treatment systems to CE seems to be very promising because it enables the automatization of the analytical process, one interesting example being included in this review [21]. In addition, these sample treatment procedures can concentrate antibiotic samples prior to the injection in the CE system.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

“…Since in alkaline media tetracyclines oxidized easily, citric acid at pH 2.5 was used for the separation of the three tretracycline antibiotics tetracycline, oxytetracycline, and doxycyline obtaining good results in terms of resolution and sensitivity. The UVabsorption of these compounds at 260 nm was registered to detect them [21].…”

Section: Tetracycline Antibioticsmentioning

confidence: 99%

See 2 more Smart Citations

Recent advances in the analysis of antibiotics by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

View full text Add to dashboard Cite

Recent advances in the analysis of antibiotics by capillary electrophoresisIn this review, the main aspects related to the separation of different groups of antibiotics by CE as well as the different applications reported in the literature from the beginning 2003 till May 2005 will be provided to the readers. Firstly, the experimental conditions employed to achieve the analysis of antibiotics by CE are given. Then, the main applications performed in the pharmaceutical, clinical, food, and environmental fields have been reviewed making emphasis on sample preparation requirements needed in each case. Finally, the main conclusions and future prospects in this field are presented. Although HPLC is mainly used for the analysis of antibiotics by separation techniques, CE is being increasingly employed due to its favorable characteristics (high efficiency, large flexibility, and low consumption of samples and reagents). In addition, CE is being used in routine analysis because it allows obtaining appropriate analytical characteristics and good quantitative results. The analysis of antibiotics by CE is mainly included in two different working modes: (i) CZE where a separation buffer without or with additives is used for the separation of ionic or ionogenic antibiotics based on their different electrophoretic mobilities, and (ii) MEKC where a micellar system (surfactant at a concentration higher than its CMC) is added to the separation buffer to perform the separation of neutral and/or ionic or ionogenic antibiotics based on the generation of a pseudostationary phase in which analyte partition takes place. Although much less used, CEC and nonaqueous CE (NACE) have also been used for the analysis of antibiotics [2]. KeywordsIn order to provide to the readers an updated view of the separation conditions as well as the different applications reported in the analysis of antibiotics using CE as separation technique, this review covers the literature dealing on the analysis of antibiotics by CE from the beginning of 2003 till May 2005. Literature published before this date on this subject has already been reviewed by Flurer [2][3][4][5]. The experimental conditions employed to achieve the analysis of antibiotics by CE are first presented in this article. Section 3 of this work describes the main applications performed in the pharmaceutical, clinical, food, and environmental fields making emphasis on sample preparation requirements needed in each case. Finally, the main conclusions and future prospects in this field are presented. Analysis of antibiotics by CE

show abstract

Section: Antibiotics In Environmental Samples: Their Role As Emergingmentioning

confidence: 99%

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Section: Tetracycline Antibioticsmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in the analysis of antibiotics by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…Different analytical techniques have been developed for determination of terbutaline sulfate and tetracycline. HPLC, 8,9 LC-MS, 10 CE, 11 CE-MS, 12 voltammetry, 13 electrochemical method, 14,15 liquid chromatography, 16,17 capillary electrophoresis 18 and chemiluminescence 19,20 have been reported for determination of tetracycline. These methods are often time-consuming, expensive, and cumbersome.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Determination of Tetracycline and Terbutaline Sulphate in Its Pure and Dosage Forms Using Eosin Y Reagent

Al-Sabha¹,

Dhamra²,

Al-Ghabsha³

2017

ECB

View full text Add to dashboard Cite

A spectrofluorimetric method has been developed for the determination of tetracycline hydrochloride and terbutaline sulphate in different dosage forms. The method is based on the quantitative quenching effect of tetracycline hydrochloride and terbutaline sulphate on the native fluorescence of Eosin Y at the pH 6.4 and 3.5 respectively. The quenching of the fluorescence of Eosin Y was measured at 545 nm after excitation at 350 nm. The fluorescence-concentration plots are rectilinear over the range 0.5-18 and 0.05-5.0 μg mL -1 with LOD of 0.531 and 0.241 μg mL -1 and LOQ of 1.77 and 0.806 μg mL -1 for above drugs respectively. The proposed method has been successfully applied to the analysis of commercial tablets and capsules containing the drug. Statistical comparison of the results with those of the reference method revealed good agreement and proved that there were no significant differences in the accuracy and precision between the two methods.* Corresponding Authors E-Mail: dr_theiaa@yahoo.co.uk [a]

show abstract

“…There are several analytical methods used to detect tetracyclines with different matrices, for example liquid-chromatography (LC) and fluorescence detection (13,24), as well as capillary electrophoresis (CE) using laser-induced fluorescence detection (21). LC with UV detection (5,7,9,16,19) has been also employed.…”

Section: Introductionmentioning

confidence: 99%

Screening method for the determination of selected tetracyclines in water by liquid chromatography with diode array detector

Patyra

Kowalczyk

Kwiatek

2014

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

A chromatographic procedure for determination of oxytetracycline (OXT), tetracycline (TC), chlorotetracycline (CTC), and doxycycline (DC) in water samples was developed and was applied for the analysis of water samples collected from poultry and pig farms and environmental water samples. Samples were acidified with trifluoroacetetic acid to pH 3 and further purified by solid phase extraction using Oasis HLB cartridges. The samples were dried up and redissolved in the mixture of oxalic acid and methanol. Separation was performed on reserved phase column (Phenomenex column C 18 , 250 mm × 4.6 mm, 5 µm) by multistep gradient elution, and detection was carried out at 360 nm for OTC and TC, 370 nm for CTC, and 350 nm for DC. The tetracyclines were eluted with the mobile phase of 0.05 M oxalic acid (pH 2.5), acetonitrile, and methanol. This method provided average recoveries of 83.53% to 108.59%, with coefficient of variations (CVs) of 2.41% to 8.64% in the range of 10 to 1000 µg/L OTC, TC, CTC, and DC in water. The linearity for the tetracyclines was determined by HPLC-DAD in the range 10 to 1000 µg/L, with the correlation coefficient (R) > 0.99. The LOD and LOQ for the tetracyclines in water samples ranged from 1.51 to 4.00 and 2.51 to 5.93 µg/L, respectively.

show abstract

Rapid determination of trace levels of tetracyclines in surface water using a continuous flow manifold coupled to a capillary electrophoresis system

Cited by 76 publications

References 18 publications

Recent advances in the analysis of antibiotics by capillary electrophoresis

Recent advances in the analysis of antibiotics by capillary electrophoresis

Spectrofluorimetric Determination of Tetracycline and Terbutaline Sulphate in Its Pure and Dosage Forms Using Eosin Y Reagent

Screening method for the determination of selected tetracyclines in water by liquid chromatography with diode array detector

Contact Info

Product

Resources

About