Rapid determinations of saccharides in high-energy drinks by short-capillary electrophoresis with contactless conductivity detection

Vochyánová, Blanka; Opekar, František; Tůma, Petr; Štulı́k, Karel

doi:10.1007/s00216-012-6242-x

Cited by 33 publications

(22 citation statements)

References 15 publications

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“…C 4 D has been also used as detection mode for the analysis of carbohydrates by CE . For instance, the quantitative and qualitative analysis of saccharides in drinks and foodstuffs is important for checking the foodstuff energy values, the control of their production procedures, the time of their storage, and possibly also for disclosing illegal additions.…”

Section: Carbohydratesmentioning

confidence: 99%

“…Under the optimal experimental conditions (75 mM sodium hydroxide at pH 12.8), the separation of the studied carbohydrates was obtained in less than 3 min with LODs lower than 1 μM. In a work by Vochyanova et al , an in‐house designed apparatus based on capillary with an effective length of 4 cm in combination with C 4 D detection was successfully applied for the rapid (less than 1 min) determination of sucrose, glucose, and fructose in high‐energy drinks. These saccharides were separated in the anionic form, in solutions of alkali hydroxides, and their determined contents were identical with the declared values within the reliability interval in most cases (RSD less than 2%).…”

Section: Carbohydratesmentioning

confidence: 99%

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Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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In this work, the analysis of foods and food components using capillary electromigration methods is reviewed. The present work presents and discusses the main CE applications performed in Food Science and Technology including the new field of Foodomics, reviewing recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food interactions and food processing. The CE analysis of a large variety of food-related molecules with different chemical properties, including amino acids, peptides, proteins, phenolic compounds, carbohydrates, DNA fragments, vitamins, toxins, pesticides, additives, and other minor compounds is described. The use of microchips, CE-MS, and chiral-CE in food analysis is also discussed as well as other current and foreseen trends in this area of research. Following the previous review by Castro-Puyana et al. (Electrophoresis, 2012, 33, 147–167), the current review covers the papers that were published from February 2011 to February 2013.

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Section: Carbohydratesmentioning

confidence: 99%

Section: Carbohydratesmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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“…This work is a continuation of a miniserie of publications describing apparatuses for electrokinetic [14][15][16] and hydrodynamic [17] injection of samples into a short separation capillary. It describes equipment for electrokinetic injection controlled by piezoelectric micropumps replacing a robust six-way injection valve and linear pump for controlling the flow of the separation electrolyte [18,19], which were used in previous designs.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic injection of samples into a short electrophoretic capillary controlled by piezoelectric micropumps

2016

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Electrokinetic sample injection using two piezoelectric micropumps has been proposed for electrophoresis in short capillaries. The sample is brought to the injection end of the capillary using one of them. Then, the high-voltage source is turned on and the sample is injected electrokinetically for a defined time. The injection is terminated by removal of the sample zone by the flowing separation electrolyte pumped by the second piezoelectric micropump. The RSD value, expressing the repeatability of the injection, does not exceed 4%. The injection apparatus does not contain any mobile mechanical components, there is no movement of the capillary and both its ends remain constantly in the solution during both the sample injection and separation. Thus, the micropumps replace the six-way injection valve and linear pump in similar types of injection apparatuses. The injection was tested in the separation and determination of ammonium and potassium ions in two samples of mineral fertilizers. The separation was performed in background electrolyte containing 500 mM of acetic acid + 20 mM Tris + 2 mM 18-crown-6 (pH 3.3) in a capillary with id 50 μm and total length/length to the contactless conductivity detector of 10.5/8 cm. The injection and separation took place at a voltage of 5 kV and the separation time equaled 20 s. The measured values of the analyte contents corresponded to the value declared by the manufacturer within the reliability interval, where RSD equaled between 3.5 and 4.7%.

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“…In 2003, Carvalho and co‐workers described a pioneering report showing the use of C 4 D to monitor separations of monosaccharides and disaccharides in conventional CE using a strongly alkali medium . Other authors have also investigated separations of carbohydrates by CE‐C 4 D . While conventional CE has emerged as a powerful technique for the separation of carbohydrates, ME approaches are seldom discussed in the literature .…”

Section: Introductionmentioning

confidence: 99%

Separation of carbohydrates on electrophoresis microchips with controlled electrolysis

et al. 2019

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This study reports the separation of fructose, galactose, glucose, lactose and sucrose on glass microchip electrophoresis (ME) devices using a microfluidic platform adapted with external reservoirs for controlling the electrolysis phenomenon. The connections between external reservoirs and microfluidic platform were performed by saline bridges created using silicone tubing filled with BGE. The separation conditions were optimized and the best results were achieved using a BGE containing 75 mmol/L NaOH and 15 mmol/L trisodium phosphate. Electrophoretic separations were monitored using a capacitively coupled contactless conductivity detection system. The controlled electrolysis has successfully allowed the application of a higher voltage on the separation channel promoting the baseline separation of five carbohydrates within 180 s with great run‐to‐run repeatability (RSD < 1%). The achieved efficiencies ranged from 45 000 ± 6000 to 70 000 ± 3000 plates/m demonstrating a performance better than ME devices without controlled electrolysis. The proposed system offered good linearity from 1 to 10 mmol/L and LODs between 150 and 740 μmol/L. The use of external tubes for controlling the electrolysis phenomenon on ME devices has solved common problems associated to run‐to‐run repeatability and analytical reliability required for routine and quantitative analysis.

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Rapid determinations of saccharides in high-energy drinks by short-capillary electrophoresis with contactless conductivity detection

Cited by 33 publications

References 15 publications

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Electrokinetic injection of samples into a short electrophoretic capillary controlled by piezoelectric micropumps

Separation of carbohydrates on electrophoresis microchips with controlled electrolysis

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