Rapid Development of Targeting circRNAs in Cardiovascular Diseases

Zhang, Chao; Huo, Sitong; Wu, Zhi‐Yong; Chen, Lina; Wen, Chang; Chen, Honghao; Du, William W.; Wu, Nan; Guan, Daogang; Lian, Sen; Yang, Burton B.

doi:10.1016/j.omtn.2020.06.022

Cited by 35 publications

(17 citation statements)

References 88 publications

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“…Accordingly, Pertuzumab significantly reduced the tumorigenicity of circHER2 and HER2–103 expressing cells in vivo. 75 In contrast, circTADA2A-E6 was downregulated in TNBC tissues and acted as a miR-203a-3p sponge. 76 Overexpression of circTADA2A-E6 suppressed cell proliferation, migration, and invasion in vitro.…”

Section: Targeting Circrnas In Diseasementioning

confidence: 99%

“…More recently, circHER2 was found to be highly expressed in TNBC samples and encoded a novel 103aa peptide, HER2–103, and played a role in tumorigenesis. 75 This peptide shared most of its amino acid sequence with the HER2 CR1 domain, which can be antagonized by Pertuzumab. Accordingly, Pertuzumab significantly reduced the tumorigenicity of circHER2 and HER2–103 expressing cells in vivo.…”

Section: Targeting Circrnas In Diseasementioning

confidence: 99%

See 1 more Smart Citation

Targeting circular RNAs as a therapeutic approach: current strategies and challenges

Liu

et al. 2021

Sig Transduct Target Ther

Self Cite

325

216

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Significant progress has been made in circular RNA (circRNA) research in recent years. Increasing evidence suggests that circRNAs play important roles in many cellular processes, and their dysregulation is implicated in the pathogenesis of various diseases. CircRNAs are highly stable and usually expressed in a tissue- or cell type-specific manner. Therefore, they are currently being explored as potential therapeutic targets. Gain-of-function and loss-of-function approaches are typically performed using circRNA expression plasmids and RNA interference-based strategies, respectively. These strategies have limitations that can be mitigated using nanoparticle and exosome delivery systems. Furthermore, recent developments show that the cre-lox system can be used to knockdown circRNAs in a cell-specific manner. While still in the early stages of development, the CRISPR/Cas13 system has shown promise in knocking down circRNAs with high specificity and efficiency. In this review, we describe circRNA properties and functions and highlight their significance in disease. We summarize strategies that can be used to overexpress or knockdown circRNAs as a therapeutic approach. Lastly, we discuss major challenges and propose future directions for the development of circRNA-based therapeutics.

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Section: Targeting Circrnas In Diseasementioning

confidence: 99%

Section: Targeting Circrnas In Diseasementioning

confidence: 99%

Targeting circular RNAs as a therapeutic approach: current strategies and challenges

Liu

et al. 2021

Sig Transduct Target Ther

Self Cite

325

216

View full text Add to dashboard Cite

show abstract

“…Certain circRNAs that are abundant and stable in exosomes are critical players in regulating cancer growth, tumour angiogenesis, and invasion [ 32 ]. They also play important roles in other diseases, such as cardiovascular diseases [ 33 ].…”

Section: Circrna Biogenesis and Functionmentioning

confidence: 99%

The Emerging Regulatory Role of Circular RNAs in Periodontal Tissues and Cells

Jiao

Walsh

Ivanovski

et al. 2021

IJMS

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Periodontitis is a chronic complex inflammatory disease associated with a destructive host immune response to microbial dysbiosis, leading to irreversible loss of tooth-supporting tissues. Regeneration of functional periodontal soft (periodontal ligament and gingiva) and hard tissue components (cementum and alveolar bone) to replace lost tissues is the ultimate goal of periodontal treatment, but clinically predictable treatments are lacking. Similarly, the identification of biomarkers that can be used to accurately diagnose periodontitis activity is lacking. A relatively novel category of molecules found in oral tissue, circular RNAs (circRNAs) are single-stranded endogenous, long, non-coding RNA molecules, with covalently circular-closed structures without a 5’ cap and a 3’ tail via non-classic backsplicing. Emerging research indicates that circRNAs are tissue and disease-specific expressed and have crucial regulatory functions in various diseases. CircRNAs can function as microRNA or RNA binding sites or can regulate mRNA. In this review, we explore the biogenesis and function of circRNAs in the context of the emerging role of circRNAs in periodontitis pathogenesis and the differentiation of periodontal cells. CircMAP3K11, circCDK8, circCDR1as, circ_0062491, and circ_0095812 are associated with pathological periodontitis tissues. Furthermore, circRNAs are expressed in periodontal cells in a cell-specific manner. They can function as microRNA sponges and can form circRNA–miRNA–mRNA networks during osteogenic differentiation for periodontal-tissue (or dental pulp)-derived progenitor cells.

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“…Circular RNAs (circRNAs) are neotype RNA molecules without 5′ to 3′ polarity produced by back-splicing ( 5 ). Mounting studies have corroborated that circRNAs are closely related to cardiovascular diseases ( 6 ), including AS. For example, down-regulation of circ_0029589 suppressed the growth and motility of ox-LDL-mediated VSMCs in AS via competitively combining with miR-424-5p to regulate IGF2 ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

circ_0003204 Regulates Cell Growth, Oxidative Stress, and Inflammation in ox-LDL-Induced Vascular Endothelial Cells via Regulating miR-942-5p/HDAC9 Axis

Wan

You

Luo

2021

Front. Cardiovasc. Med.

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Background: Atherosclerosis (AS) is a typical inflammatory vascular disease. Many reports corroborated that circular RNAs (circRNAs) is involved in AS progression. However, the potential function and possible mechanism of circ_0003204 in AS progression remain indistinct.Methods: Expression level analysis was performed using qRT-PCR and western blot. Cell viability and apoptosis were determined using Cell Counting Kit-8 (CCK-8), flow cytometry, and western blot assays. The status of oxidative stress and inflammation was determined via commercial detection kits and ELISA assay, respectively. The binding relationship was verified via dual-luciferase reporter and RNA immunoprecipitation assays.Results: ox-LDL increased circ_0003204 and HDAC9 levels and decreased miR-942-5p level. Silencing of circ_0003204 enhanced cell viability and inhibited cell apoptosis, oxidative stress and inflammation in ox-LDL-disposed HUVECs. In addition, circ_0003204 targeted miR-942-5p to regulate ox-LDL-resulted HUVECs injury. Also, miR-942-5p affected ox-LDL-triggered HUVECs injury by targeting HDAC9. Furthermore, circ_0003204 elevated HDAC9 expression via decoying miR-942-5p.Conclusion: circ_0003204 aggravated ox-LDL-induced HUVECs damage via modulating miR-942-5p/HDAC9 pathway.

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Rapid Development of Targeting circRNAs in Cardiovascular Diseases

Cited by 35 publications

References 88 publications

Targeting circular RNAs as a therapeutic approach: current strategies and challenges

Targeting circular RNAs as a therapeutic approach: current strategies and challenges

The Emerging Regulatory Role of Circular RNAs in Periodontal Tissues and Cells

circ_0003204 Regulates Cell Growth, Oxidative Stress, and Inflammation in ox-LDL-Induced Vascular Endothelial Cells via Regulating miR-942-5p/HDAC9 Axis

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