Rapid diagnosis of chlamydial infections with the MicroTrak direct test

Uyeda, Charles T.; Welborn, P; Ellison-Birang, N; Shunk, Kenneth; Tsaouse, B

doi:10.1128/jcm.20.5.948-950.1984

Cited by 95 publications

(16 citation statements)

References 6 publications

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“…It is of concern that reexamination of false-negative MT smears by using x 1,000 magnification revealed 10 to 25 organisms in eight patients, suggesting the x400 screening could miss positive smears, particularly if the microscopist is inexperienced or relatively small numbers of organisms are present. The overall results for sensitivity and specificity appear to differ significantly from those obtained by other investigators (5)(6)(7)(8). However, when the data were analyzed by population, a close correlation between culture and MT results was observed in the population with a high prevalence for chlamydial infections, again confirming the utility of the MT in patient populations with high chlamydial infection rates.…”

contrasting

confidence: 49%

Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens

Forbes¹,

Bartholoma²,

McMillan³

et al. 1986

J Clin Microbiol

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The MicroTrak Chlamydia trachomatis Direct Specimen Test (MT; Syva Co., Palo Alto, Calif.) was compared with cell culture in two patient populations. The sensitivity of the MT for a low-prevalence group was significantly lower (59.6%) than that for a high-prevalence group (84.4%). The results underscored the need to run the MT in parallel with culture initially if the prevalence of chlamydial infections is unknown and questioned the usefulness of the MT as a screening test for chlamydia in low-prevalence populations.

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contrasting

confidence: 49%

Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens

Forbes¹,

Bartholoma²,

McMillan³

et al. 1986

J Clin Microbiol

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show abstract

“…An agreement of 86.0% to 96.0% between IF tests and isolation has been demonstrated for urogenital samples (8,18,24,26). In our study, the IF test showed a low sensitivity compared to isolation.…”

Section: Resultssupporting

confidence: 53%

Evaluation of chlamydial tests in early trachoma

Elbagir

Mårdh

1990

APMIS

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An enzyme linked immunosorbent assay (ELISA) and an immunofluorescence (IF) test were compared with culture for their value in the diagnosis of chlamydial infection in early trachoma. Conjunctival swabs and scrapings from 81 school children with active trachoma from Khartoum, Sudan, were tested. Fourteen (17.3%) of the children with signs of active trachoma were positive by isolation. The sensitivities of the ELISA and IF tests, compared to cultures, were 85.0% and 68.2%, while the specificities for the ELISA and IF tests were 99.2% and 99.3%, respectively. Chlamydial IgM and IgG antibodies reactive with serotypes A‐C and D‐K of Chlamydia trachomatis were detected in sera of 28 (71.2%) of the 39 children from whom serum was obtained. Only eight of the 14 isolation‐positive children were tested for chlamydial antibodies, and all of them had IgM and IgG antibodies at a titer of ≥ 1:16. An IgG antibody titer of 1:16 or greater was detected in 20 (65.0%) of the 31 isolation‐negative patients tested for chlamydial antibodies. The geometric mean titers of IgG antibodies to the antigen pools A‐C and D‐K were 132 and 42, respectively. Our study suggests that trachoma is prevalent in the child population studied. Clinical signs of active trachoma were found in 47.0% of the altogether 172 children investigated. ELISA can be recommended as a diagnostic tool in trachoma field surveys.

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“…The Mikro-Trak assay has been extensively compared with cell culture in previous reports and its sensitivity ranges between 50-990/0 (2, 3, 8, 16) but, as far as we know, there is no comparative study done with Chlamyset. We found this test to be quite sensitive and highly specific compared with cell culture in patients seeking gynecologic care.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a direct fluorescent antibody test for detection of Chlamydia trachomatis in endocervical specimens

Dereli

Ertem

Serter

et al. 1991

APMIS

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Evaluation of a direct fluorescent antibody test for detection of Chlamydia trachomatis in endocervical specimens. APMIS 99: 961-964, 1991.A total of 160 endocervical specimens collected from 72 symptomatic and 82 asymptomatic women attending a gynecology outpatient clinic were investigated for genital Chlamydia trachomatis infection by the use of tissue culture and DFA test. The infection rate was 42% for symptomatic and 23% for asymptomatic patient groups. The sensitivity rates of the DFA test in the symptomatic and asymptomatic groups were 84% and 75' 56, while the specificity rates were 89% and 95%, respectively. The DFA test had an overall sensitivity of 80% and specificity of 93%. There was 90% agreement between the two techniques. Therefore, DFA is recommended as an alternative to tissue culture where laboratory facilities are limited and genital chlamydia1 infections are highly prevalent.

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Rapid diagnosis of chlamydial infections with the MicroTrak direct test

Cited by 95 publications

References 6 publications

Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens

Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens

Evaluation of chlamydial tests in early trachoma

Evaluation of a direct fluorescent antibody test for detection of Chlamydia trachomatis in endocervical specimens

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