Rapid diagnosis of COVID-19vianano-biosensor-implemented biomedical utilization: a systematic review

Abstract

This review will focus on the rapid, selective, accurate, easy, affordable, versatile, and point-of-care diagnosis of COVID-19 using electrochemical, optical, magnetic, aptameric, and plasmonic nano-biosensors.

Korona Virüs Tanısı ve Biyosensörler

“…Covid-19 biyosensörleri tüm vücut sıvılarında ölçüm yapabilmektedir ve mediatör gibi maddelerin kullanımıyla beraber halihazırda kullanılan yöntemlere göre tayin alt sınırları daha düşüktür. Bu özelliğinden dolayı bu yöntem hem Rt-PCR'ın güvenilirliğine sahip, hem de hızlı tanı testlerinin hızına sahip bir yöntem olarak umut vadetmektedir 3,29 .…”

“…Literatürde tanı ve tedavide önemli biyolojik moleküllerin analizini yapabilen çeşitli biyosensör çalışmaları mevcuttur. Günümüzde biyosensörler, medikal tanı aracı olmak üzere birçok amaç için hem laboratuvar tıbbında, hem de sanayide kullanılmaktadır [2][3][4]…”

Noble Metal Nanoparticles for Point-of-Care Testing: Recent Advancements and Social Impacts

“…The importance of POC testing lies in its ability to provide the early detection of infectious and noninfectious diseases alike. The ongoing coronavirus disease 2019 (COVID-19) pandemic highlights the critical need for POC testing [ 12 , 13 , 14 , 15 ]. Many laboratory tests, such as polymerase chain reaction (PCR) and mass spectroscopy, are tedious, expensive, and require trained professionals to operate the tests.…”

Nanotechnology meets immunology towards a rapid diagnosis solution: the COVID-19 outbreak challenge

“…9,13,14 In this view, the development of simple and rapid LFA-based diagnostic tests for the direct detection of SARS-CoV-2, abbreviating the usual time window necessary for conventional diagnostic methods associated with the potential utilization at the POC, is exceedingly needed as a crucial weapon to fight against the COVID-19 pandemic. 1,9,11,15,21–23…”

“…9,13,14 In this view, the development of simple and rapid LFA-based diagnostic tests for the direct detection of SARS-CoV-2, abbreviating the usual time window necessary for conventional diagnostic methods associated with the potential utilization at the POC, is exceedingly needed as a crucial weapon to ght against the COVID-19 pandemic. 1,9,11,15,[21][22][23] Herein, we present all stages of the development of an LFAbased diagnostic immunoassay for directly detecting SARS-CoV-2, encompassing the in silico design of novel synthetic peptides with immunogenic epitopes inspired by the virus spike-protein, the production of high-affinity antibodies through semi-puried hyperimmune sera from rabbits, the synthesis of colloidal gold nanoparticles as optically active nanoprobes, their bioconjugation with the affinity biomolecules, which were all integrated into a nitrocellulose membrane strip, demonstrating effective recognition of COVID-19-infected patient sera.…”