Rapid Diagnosis of Cytomegalovirus Infection by in-Situ Hybridisation in Liver Grafts

Naoumov, NikolaiV.; O’Grady, Joseph P.; Portmann, B; Alexander, G.; Aldis, Peter; Williams, Roger

doi:10.1016/s0140-6736(88)92180-0

Cited by 43 publications

(19 citation statements)

References 16 publications

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“…Furthermore, in all these cases, CMV was briefly excreted by the patients in the urine, or serology converted afterwards. This may mean that the RA is more rapid and sensitive than conventional cell culture Other methods which have been used for rapid ident ification of CMV in clinical specimens, include DNA probe technology [7,8] and shell vial cell culture, in which different clinical and tissue specimens are inoculated by centrifugation, followed by an immunofluorescence as say to detect CMV early nuclear antigen [5,9,10]. The DNA probe techniques for detection of CMV are still a sparsely available and a highly specialized tool.…”

Section: Discussionmentioning

confidence: 99%

A Prospective Study on a Rapid Method for Diagnosing Cytomegalovirus Infections in Immunosuppressed Patients

Aguado¹,

Gómez²,

Rodríguez³

et al. 1989

Nephron

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A rapid assay is described for detection of cytomegalovirus in peripheral blood lymphocytes. It consists of an indirect immunofluorescence technique for detection of cytomegalovirus antigens by means of a monoclonal antibody directed against early viral coded proteins. This assay was compared with the conventional cell culture system. Patients transplanted between December 1986 and May 1988 were studied, and of 27 patients identified, two were excluded due to early graft failure. A total of 320 blood specimens obtained were studied, and from 12 patients cytomegalovirus was isolated in at least one specimen by conventional cell culture (in total 25 specimens). Results were available with the new technique within 6 h, whereas the cell culture took an average of 13 days to develop the typical cytopathic effect changes. Sensitivity and specificity compared with that of viral isolation in conventional cell culture was 92 % and 95 %, respectively. This technique provides an accurate and rapid diagnosis of cytomegalovirus infections, and allows specific antiviral therapy to be started earlier.

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Section: Discussionmentioning

confidence: 99%

A Prospective Study on a Rapid Method for Diagnosing Cytomegalovirus Infections in Immunosuppressed Patients

Aguado¹,

Gómez²,

Rodríguez³

et al. 1989

Nephron

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“…Small granulomas are oc- casionally seen. Specific immunostaining or in situ hybridization for cytomegalovirus may be undertaken in cases where viral inclusions are absent [14][15][16], Smudgy eosinophilic intranu clear inclusions may be present within hepatocytes in adenovirus and herpes virus infec tions, the latter producing bland areas of necro sis with little or no inflammation.…”

Section: Cytomegalovirus and Non-hepatitis-virus Infectionsmentioning

confidence: 99%

Pathology of AIDS-Related Liver Disease

Lefkowitch

1994

Dig Dis

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Hepatomegaly and abnormalities of serum liver tests are common problems in patients with acquired immune deficiency syndrome. Opportunist infections (Mycobacterium avium-intracellulare and cytomegalovirus) and neoplasms (lymphoma, Kaposi’s sarcoma) are among the most prevalent hepatic lesions in AIDS. Although Kupffer cells and endothelial cells are potential sites of human immunodeficiency virus 1 (HIV-1) infection, current studies do not indicate that the liver is a major reservoir for this virus. Drug hepatotoxicity, multimicrobial infections of the biliary tree resembling sclerosing cholangitis and a variety of nonspecific hepatic changes should be considered in evaluating AIDS patients or HIV-1-infected patients with evidence of liver dysfunction.

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“…It was also present in all 18 patients with uncomplicated cytomegalovirus infection but was not identified in any of 10 subjects with transplants who had neither complication. Nine of the patients in this series underwent a diagnostic liver biopsy at 1 wk and subsequently had cytomegalovirus infection develop; cytomegalovirus DNA was identified in liver tissue of all nine patients, indicating that cytomegalovirus replication commences at an early stage.…”

mentioning

confidence: 92%

Cytomegalovirus Infection Persists in the Liver Graft in the Vanishing Bile Duct Syndrome

et al. 1992

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Cytomegalovirus infection is one factor implicated in the cause of the vanishing bile duct syndrome complicating liver transplantation. To further investigate the role of cytomegalovirus in this syndrome, we studied serial liver biopsy material by in situ hybridization for cytomegalovirus DNA using a highly sensitive technique that allows the localization of viral replication. Cytomegalovirus DNA was identified in hepatocytes in 10 of 12 patients with the vanishing bile duct syndrome, 1 of whom had no serological evidence of cytomegalovirus infection. It was also present in all 18 patients with uncomplicated cytomegalovirus infection but was not identified in any of 10 subjects with transplants who had neither complication. Nine of the patients in this series underwent a diagnostic liver biopsy at 1 wk and subsequently had cytomegalovirus infection develop; cytomegalovirus DNA was identified in liver tissue of all nine patients, indicating that cytomegalovirus replication commences at an early stage. In those with uncomplicated cytomegalovirus, infection occurred earlier (p less than 0.05) but was eliminated more quickly (p less than 0.0005), and the number of infected hepatocytes was greater (p less than 0.05) when compared with those with the vanishing bile duct syndrome; in these, cytomegalovirus DNA was detectable until death or retransplantation. Cytomegalovirus DNA was never identified in either biliary or endothelial tissue. These data indicate that the vanishing bile duct syndrome is associated with persistent cytomegalovirus replication within hepatocytes.

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Rapid Diagnosis of Cytomegalovirus Infection by in-Situ Hybridisation in Liver Grafts

Cited by 43 publications

References 16 publications

A Prospective Study on a Rapid Method for Diagnosing Cytomegalovirus Infections in Immunosuppressed Patients

A Prospective Study on a Rapid Method for Diagnosing Cytomegalovirus Infections in Immunosuppressed Patients

Pathology of AIDS-Related Liver Disease

Cytomegalovirus Infection Persists in the Liver Graft in the Vanishing Bile Duct Syndrome

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