Rapid diagnosis of largemouth bass ranavirus in fish samples using the loop-mediated isothermal amplification method

Zhu, Qinchao; Wang, Yi; Feng, Jia

doi:10.1016/j.mcp.2020.101569

Cited by 27 publications

(12 citation statements)

References 19 publications

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“…These genome studies have greatly elevated our understanding about genetics, environmental adaptive selection, and evolutionary history of the target species. These more detailed genomic data can also facilitate studies on nutritional requirements, disease control and prevention, and to improve traits of economic interest 25 – 27 .…”

Section: Background and Summarymentioning

confidence: 99%

Chromosome-level genome assembly of largemouth bass (Micropterus salmoides) using PacBio and Hi-C technologies

Zhao

Yuan

et al. 2022

Sci Data

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The largemouth bass (Micropterus salmoides) has become a cosmopolitan species due to its widespread introduction as game or domesticated fish. Here a high-quality chromosome-level reference genome of M. salmoides was produced by combining Illumina paired-end sequencing, PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. Ultimately, the genome was assembled into 844.88 Mb with a contig N50 of 15.68 Mb and scaffold N50 length of 35.77 Mb. About 99.9% assembly genome sequences (844.00 Mb) could be anchored to 23 chromosomes, and 98.03% assembly genome sequences could be ordered and directed. The genome contained 38.19% repeat sequences and 2693 noncoding RNAs. A total of 26,370 protein-coding genes from 3415 gene families were predicted, of which 97.69% were functionally annotated. The high-quality genome assembly will be a fundamental resource to study and understand how M. salmoides adapt to novel and changing environments around the world, and also be expected to contribute to the genetic breeding and other research.

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Section: Background and Summarymentioning

confidence: 99%

Chromosome-level genome assembly of largemouth bass (Micropterus salmoides) using PacBio and Hi-C technologies

Zhao

Yuan

et al. 2022

Sci Data

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“…It is too demanding for experimenters and cannot quantitatively analyse the virus, which cannot meet the needs of rapid detection. For decades, various nucleic acid‐based assays have been widely used for the specific detection of LMBV, including conventional PCR (Deng et al., 2011; Dong et al., 2017; Grizzle et al., 2003; Jeong et al., 2006; Mao et al., 1997, 1999), quantitative PCR (qPCR) (Getchell et al., 2007; Groocock et al., 2008; Ma et al., 2011) and Loop‐Mediated Isothermal Amplification (LAMP) (Zhu et al., 2020a; Zhu, Zeng, et al., 2020). PCR targeting the major capsid protein (MCP) gene of the virus was frequently used for detection of LMBV (Getchell et al., 2007; Ma et al., 2010).…”

Section: Introductionmentioning

confidence: 99%

Establishment and evaluation of qPCR and real‐time recombinase‐aided amplification assays for detection of largemouth bass ranavirus

Guo

Wang²,

Zhang

et al. 2022

Journal of Fish Diseases

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Largemouth bass (Micropterus salmoides) is a high-grade freshwater aquaculture species (Li & Wang, 2016). In the recent decade, largemouth bass aquaculture industry has achieved rapid development in China, and its production has increased at an annual rate of more than 9%. According to the data of China Fishery Statistics Yearbook of 2020, the national production of largemouth bass is about 478,000 tons with an output value of over ten billion Yuan (Zhang et al., 2021).Disease problems of largemouth bass are escalating with the increase of farming scale, stocking density, and the shortage of water

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“…The production of largemouth bass has reached 0.4778 million tons in China in 2019 [ 3 ]. However, largemouth bass usually suffers from disease problems, especially caused by viruses such as largemouth bass virus (LMBV), which belongs to the genus Ranavirus, family Iridoviridae and has been recognized as one of the major pathogens in largemouth bass worldwide [ 4 , 5 ]. Typically, LMBV infection causes lethargy, external and internal hemorrhages, and organomegaly, resulting in high mortality and serious economic losses [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Undoubtedly, the early and rapid diagnosis of LMBV is critical for controlling LMBV infection. Presently, PCRs are commonly used methods to detect LMBV, including conventional PCR, qRT-PCR and LAMP [ 5 ]. These methods are sensitive but complex procedures, while expensive equipment and high environmental requirement limit their application in largemouth bass aquaculture [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Sandwich ELASA Based on Aptamer for Detection of Largemouth Bass Virus (LMBV)

Zhang

et al. 2022

Viruses

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Largemouth bass virus (LMBV) is a major viral pathogen in largemouth bass culture, usually causing high mortality and heavy economic losses. Accurate and early detection of LMBV is crucial for diagnosis and control of the diseases caused by LMBV. Previously, we selected the specific aptamers, LA38 and LA13, targeting LMBV by systematic evolution of ligands by exponential enrichment (SELEX). In this study, we further generated truncated LA38 and LA13 (named as LA38s and LA13s) with high specificity and affinities and developed an aptamer-based sandwich enzyme-linked apta-sorbent assay (ELASA) for LMBV diagnosis. The sandwich ELASA showed high specificity and sensitivity for the LMBV detection, without cross reaction with other viruses. The detection limit of the ELASA was as low as 1.25 × 102 LMBV-infected cells, and the incubation time of the lysate and biotin labeled aptamer was as short as 10 min. The ELASA could still detect LMBV infection in spleen lysates at dilutions of 1/25, with good consistency of qRT-PCR. For the fish samples collected from the field, the sensitivity of ELASA was 13.3% less than PCR, but the ELASA was much more convenient and less time consuming. The procedure of ELASA mainly requires washing and incubation, with completion in approximately 4 h. The sandwich ELASA offers a useful tool to rapidly detect LMBV rapidly, contributing to control and prevention of LMBV infection.

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Rapid diagnosis of largemouth bass ranavirus in fish samples using the loop-mediated isothermal amplification method

Cited by 27 publications

References 19 publications

Chromosome-level genome assembly of largemouth bass (Micropterus salmoides) using PacBio and Hi-C technologies

Chromosome-level genome assembly of largemouth bass (Micropterus salmoides) using PacBio and Hi-C technologies

Establishment and evaluation of qPCR and real‐time recombinase‐aided amplification assays for detection of largemouth bass ranavirus

A Novel Sandwich ELASA Based on Aptamer for Detection of Largemouth Bass Virus (LMBV)

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