Rapid Diagnosis of M. tuberculosis Using a Piezoelectric Immunosensor

He, Fengjiao; Zhang, Liude

doi:10.2116/analsci.18.397

Cited by 48 publications

(23 citation statements)

References 17 publications

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“…There are several studies linking the use of the Quartz Crystal Microbalance (QCM) to the detection of diseases characterizing biological fluids as blood [12,13,14,15], urine [16,17], saliva [17,18,19] and synovial fluid [20,21]. The methods proposed in these studies have proven to be successful according to their results.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Hyaluronic Acid Dilutions at Different Concentrations Using a Quartz Crystal Resonator (QCR) for the Potential Diagnosis of Arthritic Diseases

Ahumada

González

Sandoval

et al. 2016

Sensors

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The main objective of this article is to demonstrate through experimental means the capacity of the quartz crystal resonator (QCR) to characterize biological samples of aqueous dilutions of hyaluronic acid according to their viscosity and how this capacity may be useful in the potential diagnosis of arthritic diseases. The synovial fluid is viscous due to the presence of hyaluronic acid, synthesized by synovial lining cells (type B), and secreted into the synovial fluid thus making the fluid viscous. In consequence, aqueous dilutions of hyaluronic acid may be used as samples to emulate the synovial fluid. Due to the viscoelastic and pseudo-plastic behavior of hyaluronic acid, it is necessary to use the Rouse model in order to obtain viscosity values comparable with viscometer measures. A Fungilab viscometer (rheometer) was used to obtain reference measures of the viscosity in each sample in order to compare them with the QCR prototype measures.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Hyaluronic Acid Dilutions at Different Concentrations Using a Quartz Crystal Resonator (QCR) for the Potential Diagnosis of Arthritic Diseases

Ahumada

González

Sandoval

et al. 2016

Sensors

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“…Other methodologies, such as piezoelectronic immunosensors or fluorescent nanoparticles coupled with flow cytometry, and nanowires are further away from application in practice [8], [47], [48]. Still, SPR equipment is hardly compatible with field settings in countries where tuberculosis diagnosis is needed most.…”

Section: Discussionmentioning

confidence: 99%

A Broad Set of Different Llama Antibodies Specific for a 16 kDa Heat Shock Protein of Mycobacterium tuberculosis

Trilling

Ronde

Noteboom³

et al. 2011

PLoS ONE

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BackgroundRecombinant antibodies are powerful tools in engineering of novel diagnostics. Due to the small size and stable nature of llama antibody domains selected antibodies can serve as a detection reagent in multiplexed and sensitive assays for M. tuberculosis.Methodology/Principal FindingsAntibodies for Mycobacterium tuberculosis (M. tb) recognition were raised in Alpaca, and, by phage display, recombinant variable domains of heavy-chain antibodies (VHH) binding to M. tuberculosis antigens were isolated. Two phage display selection strategies were followed: one direct selection using semi-purified protein antigen, and a depletion strategy with lysates, aiming to avoid cross-reaction to other mycobacteria. Both panning methods selected a set of binders with widely differing complementarity determining regions. Selected recombinant VHHs were produced in E. coli and shown to bind immobilized lysate in direct Enzymelinked Immunosorbent Assay (ELISA) tests and soluble antigen by surface plasmon resonance (SPR) analysis. All tested VHHs were specific for tuberculosis-causing mycobacteria (M. tuberculosis, M. bovis) and exclusively recognized an immunodominant 16 kDa heat shock protein (hsp). The highest affinity VHH had a dissociation constant (KD) of 4×10−10 M.Conclusions/SignificanceA broad set of different llama antibodies specific for 16 kDa heat shock protein of M. tuberculosis is available. This protein is highly stable and abundant in M. tuberculosis. The VHH that detect this protein are applied in a robust SPR sensor for identification of tuberculosis-causing mycobacteria.

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“…It is based on the frequency shift caused by antigen binding to rabbit IgG against the TB bacterium, immobilized in an ordered orientation onto the crystal surface. 103 Detection was performed within the range of 10 5 -10 8 cells/mL. Other methods such as the bulk acoustic-wave impedance biosensor 104 and the multichannel series piezoelectric quartz crystal sensor system, 105 have been developed for rapid detection of Mycobacterium tuberculosis.…”

Section: Tuberculosismentioning

confidence: 99%

Biosensors as rapid diagnostic tests for tropical diseases

Teles

Tavira

Fonseca

2010

Critical Reviews in Clinical Laboratory Sciences

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Effective diagnosis of infectious pathogens is essential for disease identification and subsequent adequate treatment, to prevent drug resistance and to adopt suitable public health interventions for the prevention and control of epidemic outbreaks. Particular situations under which medical diagnostics operate in tropical environments make the use of new easy-to-use diagnostic tools the preferred (or even unique) option. These diagnostic tests and devices, usually based on biosensing methods, are being increasingly exploited as promising alternatives to classical, "heavy" lab instrumentation for clinical diagnosis, allowing simple, inexpensive and point-of-care testing. However, in many developing countries the lack of accessibility and affordability for many commercial diagnostic tests remains a major cause of high disease burden in such regions. We present a comprehensive overview about the problems of conventional medical diagnosis of infectious pathologies in tropical regions, while pointing out new methods and analytical tools for in-the-field and decentralized diagnosis of current major infectious tropical diseases. The review includes not only biosensor-based rapid diagnostic tests approved by regulatory entities and already commercialized, but also those at the early stages of research.

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Rapid Diagnosis of M. tuberculosis Using a Piezoelectric Immunosensor

Cited by 48 publications

References 17 publications

Evaluation of Hyaluronic Acid Dilutions at Different Concentrations Using a Quartz Crystal Resonator (QCR) for the Potential Diagnosis of Arthritic Diseases

Evaluation of Hyaluronic Acid Dilutions at Different Concentrations Using a Quartz Crystal Resonator (QCR) for the Potential Diagnosis of Arthritic Diseases

A Broad Set of Different Llama Antibodies Specific for a 16 kDa Heat Shock Protein of Mycobacterium tuberculosis

Biosensors as rapid diagnostic tests for tropical diseases

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