Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report

Xing, Fanfan; Lo, Stephen T.H.; Ma, Yuanchao; Ip, Jonathan Daniel; Chan, Wan-Mui; Zhou, M.; Gong, Miaozi; Lau, Susanna K. P.; Woo, Patrick C. Y.

doi:10.3389/fmed.2022.824122

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…The use of the NGS technology in fungal pathogen detection offers the following benefits: First, NGS technology is suitable for hostile culture and slow-growing microbial infections, such as fungi [ 33 ]. It will be a useful tool for low fungal loads [ 19 ].…”

Section: Ngs and Fungal Sequencingmentioning

confidence: 99%

Next-Generation Sequencing Applications for the Study of Fungal Pathogens

Jiang

Chen

et al. 2022

Microorganisms

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Next-generation sequencing (NGS) has become a widely used technology in biological research. NGS applications for clinical pathogen detection have become vital technologies. It is increasingly common to perform fast, accurate, and specific detection of clinical specimens using NGS. Pathogenic fungi with high virulence and drug resistance cause life-threatening clinical infections. NGS has had a significant biotechnological impact on detecting bacteria and viruses but is not equally applicable to fungi. There is a particularly urgent clinical need to use NGS to help identify fungi causing infections and prevent negative impacts. This review summarizes current research on NGS applications for fungi and offers a visual method of fungal detection. With the development of NGS and solutions for overcoming sequencing limitations, we suggest clinicians test specimens as soon as possible when encountering infections of unknown cause, suspected infections in vital organs, or rapidly progressive disease.

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Section: Ngs and Fungal Sequencingmentioning

confidence: 99%

Next-Generation Sequencing Applications for the Study of Fungal Pathogens

Jiang

Chen

et al. 2022

Microorganisms

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“…diarrhea) plus Rifampin, then Ethambutol plus Rifampin (disc. resistance), then Ethambutol plus Rifabutin 5 months Cure Xing et al 22 M/38 China Owner of a seafood market, fish bite Right middle finger, right hand and elbow Lymphocutaneous form poorly healed primary wound on the right middle finger, secondary ulcerated mass on right hand, subcutaneous nodule at the right elbow and supratrochlear lymph nodes Rifampin plus Minocycline plus Clarithromycin 9 months Cure Vanhooteghem et al 23 M/41 Belgium Fish manicure-Thailand vacation Left thumb Hardened, rough, brownish erythematous periungual plaque Doxycycline 4 months Cure Trčko et al 24 M/37 Slovenia Aquarium owner (tropical fish, exotic snakes, lizards) Right hand Small warty papule progressed to erythematous scaling plaque on the fourth finger, after a few months a new lesion appeared on the back of his hand and the extensor side of the right wrist. Rifampicin plus Clarithromycin 6 months Cure Wenlong et al 25 M/18 China Chef Left hand Plaques and nodules Photodynamic therapy combined with fractional CO 2 laser ablation ...…”

Section: Discussionmentioning

confidence: 99%

Acid-fast bacteria as causative agents of skin and soft tissue infections: case presentations and literature review

Spiliopoulou

Kyriakou

Georgiou

et al. 2023

Rev. Inst. Med. trop. S. Paulo

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Acid-fast bacteria can be implicated in skin and soft tissue infections. Diagnostic identification can be challenging or not feasible by routine laboratory techniques, especially if there is no access to the Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) technology. Here, we present two cases of skin and soft tissue infections caused by two different acid-fast bacteria, Nocardia brasiliensis and Mycobacterium marinum . They both grew on Löwenstein–Jensen medium, Sabouraud agar medium and blood agar medium. Both bacteria appeared acid-fast by Ziehl–Neelsen stain and Gram-positive by Gram stain. The identification was performed by MALDI-TOF MS and gene analysis. N. brasiliensis and nontuberculous mycobacterium M. marinum represent rare pathogens that cause severe skin and soft tissue infections. Failure to identify the causative agent and subsequent inappropriate or inadequate treatment may lead to severe complications or even disseminated disease, especially in immunocompromised individuals.

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“…In the clinical setting for infectious diseases, NGS is used most often for patients who have fever without localizing features or culture-negative infections. We have recently reported its application in fungal diagnosis as well as confirming the first case of listeria meningitis in a patient with autoantibody against interferon gamma and another one with Mycobacterium marinum infection [ 17 – 19 ]. In the present cohort, case 2 and 3 both presented with fever and severe headache and were admitted to the neurology unit as suspected meningitis.…”

Section: Discussionmentioning

confidence: 99%

Diverse and atypical manifestations of Q fever in a metropolitan city hospital: Emerging role of next-generation sequencing for laboratory diagnosis of Coxiella burnetii

et al. 2022

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Although Q fever has been widely reported in the rural areas of China, there is a paucity of data on the epidemiology and clinical characteristics of this disease in large metropolitan cities. In this study, we profile the epidemiology and clinical manifestations of Q fever from a tertiary hospital in Shenzhen, a Southern Chinese metropolitan city with a large immigrant population from other parts of China. A total of 14 patients were confirmed to have Q fever during a nine-year-and-six-month period, five of whom were retrospectively diagnosed during case review or incidentally picked up because of another research project on unexplained fever without localizing features. Some patients had the typical exposure histories and clinical features, while a few other patients had rare manifestations of Q fever, including one with heart failure and diffuse intracapillary proliferative glomerulonephritis, a patient presenting with a spontaneous bacterial peritonitis-like syndrome, and another one with concomitant Q fever and brucellosis. Using a combination of clinical manifestation, inflammatory marker levels, echocardiographic findings and serological or molecular test results, nine, three and two patients were diagnosed to have acute, chronic and convalescent Q fever, respectively. Seven, five and two patients were diagnosed to have Q fever by serological test, nested real-time PCR and next-generation sequencing respectively. Diverse and atypical manifestations are associated with Q fever. The incidence of Q fever is likely to be underestimated. Next-generation sequencing is becoming an important diagnostic modality for culture-negative infections, particularly those that the physicians fail to recognize clinically, such as Q fever.

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Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report

Cited by 12 publications

References 20 publications

Next-Generation Sequencing Applications for the Study of Fungal Pathogens

Next-Generation Sequencing Applications for the Study of Fungal Pathogens

Acid-fast bacteria as causative agents of skin and soft tissue infections: case presentations and literature review

Diverse and atypical manifestations of Q fever in a metropolitan city hospital: Emerging role of next-generation sequencing for laboratory diagnosis of Coxiella burnetii

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