Rapid diagnosis of oral tuberculosis by amplification of Mycobacterium DNA from paraffin embedded specimens.

Abstract

Abstract:The polymerase chain reaction (PCR) assay is a powerful tool for quick diagnosis of various infectious diseases. We applied this technique as well as conventional histopathological examination to diagnose oral tuberculosis. Ziehl-Neelsen staining of oral mucosal specimens often fails to detect Mycobacterium (M.) tuberculosis due to the low number of bacteria in the tissue. Specific primers and probes were synthesized based upon the nucleotide sequence of the 65 kDa membrane protein of M. tuberculosis.DNA extracted from the paraffinembedded tissue was amplified using tali polymerase. PCR assay detected M. tuberculosis in 5 of 6 samples. Although the gene segments from these species were quite similar, the 732P labeled noligonucleotide probes distinguished between M. tuberculosis and M. fotuitum by southern blot hybridization. In all specimens that were Ziehl-Neelsen negative, M. tuberculosis DNA was detected by PCR. These results suggest that PCR is a useful means of diagnosing mycobacterium infection. (J. Oral Sci. 40, 31-36, 1998)

Submandibular Tuberculous Lymphadenitis after Endodontic Treatment of the Mandibular First Premolar Tooth: Report of a Case

“…Komiyama et al (8) reported that Ziehl-Neelsen staining of oral mucosal specimens often fails to detect M. tuberculosis because of the low number of bacteria in the tissue. Because PCR had enabled a rapid and simple diagnostic system for the detection of M. tuberculosis (5)(6)(7)(8), in our study, PCR was used in addition to conventional methods.…”

“…For the detection of Mycobacterium tuberculosis in clinical samples, the following testing procedures were carried out: Polymerase chain reaction (PCR), which is rapid and reliable technique (5)(6)(7)(8), Löwenstein-Jensen (LJ), direct smear, and EZN (Erlich-ZiehlNeelsen staining).…”

Ulcération linguale avec dysphagie à l’origine du diagnostic d’une tuberculose pulmonaire

“…La méthode classique consiste en la mise en évidence de bacilles acido-alcoolo-résistants (BAAR) par l'examen microscopique (après coloration de Ziehl-Neelsen) ou la mise en culture sur milieu de Lowenstein-Jensen (résultats après 2-4 semaines). De nouvelles méthodes permettent d'obtenir des résultats plus rapidement : détection radiométrique en milieu liquide, techniques d'hybridation avec sondes nucléiques spécifiques, amplification génique par PCR [9][10][11]. La présence de BAAR à l'examen microscopique direct d'au moins 2 frottis d'expectoration est le signe d'une contagiosité maximale.…”