Rapid Diagnosis of Tuberculosis by Amplification of Mycobacterial Dna in Clinical Samples

Brisson-Noël, A; Lecossier, Denise; Nassif, Xavier; Gicquel, Brigitte; Lévy-Frébault, Véronique; Hance, AllanJ.

doi:10.1016/s0140-6736(89)91082-9

Cited by 357 publications

(165 citation statements)

References 9 publications

Supporting

Mentioning

156

Contrasting

Unclassified

Order By: Relevance

“…Nonviable mycobacteria could have been originated from patients who had undergone antimycobacterial therapy. This is consistent with the fact that treated patients can still harbour Mycobacteria long after culture has become negative (Brisson-Noel et al 1989;Leeve et al 1994). In addition, decontamination during sample processing could also possibly reduce the viability of mycobacteria (Pao 1988) and consequently, the efficiency of culture.…”

Section: Discussionsupporting

confidence: 73%

Evaluation of Amplicor‐ and IS6110‐PCR for direct detection of Mycobacterium tuberculosis complex in Singapore

Thoe

Tay

Sng

1997

Tropical Med Int Health

View full text Add to dashboard Cite

SummaryOne hundred and seventy-eight samples from 168 individuals were tested for Mycobacterium tuberculosis complex (Mtc) using Amplicor PCR, IS6110-PCR (in-house), acid fast (AF)-staining and culture. Thirty-one samples were positive by culture, but 37 samples were later resolved to be truly positive for Mtc. Of these, Amplicor detected 32 (86.5%), IS6110-PCR detected 31 (83.6%), and AF-staining 21 (56.8%). None of the 141 Mtc-negative samples was positive by these tests, thus giving 100% specificity. Although the IS6110-PCR was more sensitive than Amplicor in detecting spiked Mtc DNA, it was not more sensitive than the latter in detecting Mtc in clinical samples. Reasons likely to account for the PCR false negativity were (i) sample inoculum size, (ii) nonuniform samples due to clumping effect of Mtc and (iii) the absence of target gene sequences for IS6110-PCR. Culture negativity, on the other hand, was likely to be associated with nonviable Mtc. Amplicor PCR is promising for direct detection of Mtc. The IS6110-PCR, however, may not be as suitable because of possible existence of IS6110-deleted Mtc strain in Singapore.

show abstract

Section: Discussionsupporting

confidence: 73%

Evaluation of Amplicor‐ and IS6110‐PCR for direct detection of Mycobacterium tuberculosis complex in Singapore

Thoe

Tay

Sng

1997

Tropical Med Int Health

View full text Add to dashboard Cite

show abstract

“…urine and Mycobacterium tuberculosis DNA from sputum and biopsy specimens (20,21). In this study, we have shown that no Yersinia DNA was detectable by PCR in synovial fluid or peripheral blood cells from DISCUSSION patients with Yersinia-triggered reactive arthritis.…”

Section: Resultsmentioning

confidence: 63%

Application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in Yersinia‐triggered reactive arthritis

Viitanen

Arstila

Lahesmaa

et al. 1991

Arthritis & Rheumatism

View full text Add to dashboard Cite

“…A PCR na detecção de micobactérias não é à prova de falhas, pois sua sensibilidade na detecção de micobactérias tem sido relatada na faixa de 55-100% [28][29][30][31] . Por outro lado, esta investigação pode ser usada quando a carga bacteriana é muito baixa 15,32 .…”

Section: Discussionunclassified

Children with significant cervical lymphadenopathy: clinicopathological analysis and role of fine-needle aspiration in Indian setup

Khan¹,

Wahab²,

Chana³

et al. 2008

J Pediatr (Rio J)

View full text Add to dashboard Cite

ResumoObjetivo: Estudar o perfil clínico-patológico de crianças indianas com linfadenopatia cervical e o papel da citologia aspirativa por agulha fina com ênfase especial na tuberculose como causa. Resultados: A hiperplasia reativa foi o tipo mais comum de linfadenite, seguida da granulomatosa. Os linfonodos do triângulo posterior unilateral foram o grupo afetado com maior freqüência no grupo de linfadenopatia cervical tuberculosa. A aspiração por agulha fina, seguida da coloração de Ziehl-Neelsen, histopatologia e cultura em associação, obteve sucesso em realizar o diagnóstico em 85,7% dos casos de etiologia tuberculosa. Métodos Conclusões:A aspiração por agulha fina é uma ferramenta diagnóstica valiosa no tratamento de crianças com apresentação clínica de linfonodos cervicais aumentados. A técnica reduz a necessidade de procedimentos mais invasivos e dispendiosos, principalmente em países em desenvolvimento.Cultura e histopatologia, entretanto, devem ser consideradas em casos nos quais a citologia aspirativa por agulha fina não é diagnóstica.J Pediatr (Rio J). 2008;84(5):449-454: Linfadenopatia cervical, citologia aspirativa por agulha fina, crianças. AbstractObjective: To study the clinicopathological profile of children from India with cervical lymphadenopathy and the role of fine-needle aspiration cytology with special emphasis on tuberculosis as a cause. Methods:A total of 89 children in the age group of 10 months to 12 years, presenting to our hospital from April 2004 to March 2005, were included. All the patients underwent thorough clinical and investigational assessment vis-à-vis cervical lymphadenopathy. Outcome measurements included clinical status and ability of conventional tests to categorize different types of lymphadenopathy and their utility in diagnosing tubercular lymphadenitis. Interobserver variability was analyzed measuring kappa test and was found to be in agreement. Results:Reactive hyperplasia was the most common type of lymphadenitis, followed by granulomatous involvement. Unilateral posterior triangle lymph nodes were the most commonly affected in the tubercular cervical lymphadenopathy group. Fine-needle aspiration followed by Ziehl-Neelsen staining, histopathology and culture in combination were able to perform the diagnosis in 85.7% of cases affected with tubercular etiology. Conclusions:Fine-needle aspiration is a valuable diagnostic tool in the management of children with the clinical presentation of enlarged cervical lymph nodes. The technique reduces the need for more invasive and costly procedures, especially in a Third World country. Culture and histopathology, however, should be considered in cases where repeated fine-needle aspiration cytology is non-diagnostic.J Pediatr (Rio J). 2008;84(5):449-454: Cervical lymphadenopathy, fine-needle aspiration cytology, children.

show abstract

Rapid Diagnosis of Tuberculosis by Amplification of Mycobacterial Dna in Clinical Samples

Cited by 357 publications

References 9 publications

Evaluation of Amplicor‐ and IS6110‐PCR for direct detection of Mycobacterium tuberculosis complex in Singapore

Evaluation of Amplicor‐ and IS6110‐PCR for direct detection of Mycobacterium tuberculosis complex in Singapore

Application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in Yersinia‐triggered reactive arthritis

Children with significant cervical lymphadenopathy: clinicopathological analysis and role of fine-needle aspiration in Indian setup

Contact Info

Product

Resources

About