Rapid diagnosis of tuberculous meningitis by polymerase chain reaction

Shankar, Prem; Manjunath, N; Shriniwas,; Mohan, Krishna; Prasad, Kameshwar; Behari, Madhuri; Gk, Ahuja

doi:10.1016/0140-6736(91)93328-7

Cited by 216 publications

(112 citation statements)

References 9 publications

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“…Two rounds of PCR amplification were performed in a Genius thermal cycler (Techne). The first round used the pair of external primers (here named MPB1 and MPB2) originally described by Shankar and colleagues 20 , while the second round used the internal primers (here named MPB3 and MPB4) designed by Liu and colleagues 13 . All primers were supplied by Gibco, and their sequences were given in the original publications.…”

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confidence: 99%

Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Brienze

et al. 2001

Rev. Soc. Bras. Med. Trop.

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Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy. Key-words: Diagnosis of tuberculous meningitis. PCR. Mycobacterium tuberculosis.Resumo Dois protocolos de reação em cadeia da polimerase (PCR) apresentaram baixa sensibilidade (36% e 53%, respectivamente), para TB AMPLICOR e PCR aninhado baseado no gene MPB64), quando comparados aos métodos microbiológicos clássicos (73% e 54% respectivamente para baciloscopia e cultura), no diagnóstico de meningite tuberculosa em 91 pacientes do sudeste do Brasil. Somente três pacientes apresentaram PCR positiva e microbiologia negativa. A análise de amostras seqüenciais de líquor com a PCR aninhada detectou DNA de Mycobacterium tuberculosis até 29 dias após a introdução de tratamento. Palavras-chaves: Diagnóstico da meningite tuberculosa. PCR. Mycobacterium tuberculosis.

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confidence: 99%

Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Brienze

et al. 2001

Rev. Soc. Bras. Med. Trop.

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“…Even tuberculomas which increase in size on medical therapy are monitored for at least two months before contemplating surgery. (Shankar et al, 1991) Prognosis and outcomes OF TBM: 1. Overall mortality in tuberculous meningitis is about 30%.…”

Section: Role Of Surgerymentioning

confidence: 99%

Adenosine Deaminase (ADA) in Tuberculous Meningitis

Skarar

Hossain

Shoab³

et al. 2015

Bangla J Med

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“…Their PCR assay detected only 4 of 9 cases. Amano et al (1993) and Shankar et al (1991) have demonstrated the high sensitivity and specificity of the PCR method using primers amplifying a 240-base pair sequence in the MPB64-coding gene to diagnose PTB and extrapulmonary TB, including tuberculous meningitis. In the present study, we evaluated the sensitivity and specificity of the PCR technique employing the same primers in patients who were suspected to have active PTB but had AFB-smear-negative samples of sputum or gastric aspirate according to several different criteria for PCR positivity.…”

Section: Dlscusszonmentioning

confidence: 99%

“…The primer pair detecting MPB64 coding gene is very popular and specificity for typical and atypical mycobacteria has been reported (Shankar et al 1990; Manjunath et al 1991;Shankar et al 1991;Uematsu et al 1992). Bacteriological specificity of PCR for detection of Mycobacterium tuberculosis complex was tested using commonly isolated non-mycobacterial DNA templates from the respiratory system; these included Staphylococcus aureus, Haemophilus inf luenzae, Moraxella cat arrhalis, Streptococcus pneumoniae, Pseudomonas aeruginosa, a-streptococcus, Candida albicans and human peripheral blood cells.…”

Section: Treatment Of Clinical Samplesmentioning

confidence: 99%

Clinical Evaluation of Polymerase Chain Reaction DNA Amplification Method for the Diagnosis of Pulmonary Tuberculosis in Patients with Negative Acid-fast Bacilli Smear.

Mitarai

Oishi

Fukasawa

et al. 1995

Tohoku J. Exp. Med.

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We evaluated the sensitivity and specificity of polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis among 109 patients who were suspected to have active pulmonary tuberculosis (PTB) and showed negative acid-fast bacilli (AFB) smears in a total of 393 samples of sputum (169), gastric aspirate (134), and urine (90) which fulfilled different criteria for the positivity of PCR. The patients were subsequently divided into one group of active PTB composed of 15 patients with definite PTB and 43 patients with highly suspected PTB, and another group of 51 non-active PTB patients. The PCR assay using samples of sputum and gastric aspirate proved to be specific for active PTB. The PCR method for diagnosis of active PTB using sputum samples was sensitive (97.8%) but lacked specificity (27.0%) when regarded as PCR positive when at least one positive reaction was obtained among all samples examined. However, the PCR of gastric aspirate demonstrated a sensitivity of 63.4% and a specificity of 76.7%. Our data supports that the PCR method for detecting active PTB in AFB smear negative patients using gastric aspirate shows markedly improved sensitivity over the conventional method (25.9%), although it still lacks specificity. PCR assay for M, tuberculosis using multiple samples of gastric aspirate in conjunction with careful clinical observations for the presence of active infection is essential for the diagnosis of active PTB among patients with negative AFB smear.polymerase chain reaction; Mycobacterium tuberculosis; negative acidfast bacilli smear; diagnosis Tuberculosis (TB) is still a serious disease world wide and is becoming even more important with the increasing number of individuals who have the acquired

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Rapid diagnosis of tuberculous meningitis by polymerase chain reaction

Cited by 216 publications

References 9 publications

Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Adenosine Deaminase (ADA) in Tuberculous Meningitis

Clinical Evaluation of Polymerase Chain Reaction DNA Amplification Method for the Diagnosis of Pulmonary Tuberculosis in Patients with Negative Acid-fast Bacilli Smear.

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