Rapid Diagnostic Detection of Plum Pox Virus by Isothermal Amplifyrp® and by Immunostrip®

Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees

“…PPV detection techniques have had significant improvements in the past few decades, starting from biological tests using indicator plants to serology assays, and then to molecular technologies [ 14 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Because serological methods are easy to use, cost effective, and most results obtained through serological tests agree with the results obtained through PCR or RT-PCR, serological methods are often considered as the most economical and reliable methods for plant virus detections, including PPV [ 14 , 37 ]. During serological tests, polyclonal antibodies (PAbs) can recognize multiple epitopes on viral proteins or virions and thus, the specificity of PAbs always create controversy [ 38 ].…”

“…Maejima and others have also reported an immunochromatographic strip using the PAb against the recombinant expressed CP of a PPV-D isolate, and the detection limit of the strip was up to 1:512 dilution for PPV-infected leaf crude extracts [ 14 ]. The commercial immunochromatographic strip made by Agdia Inc. (USA) can be used to detect PPV-D in 1:100 ( w / v ) diluted infected plant tissue crude extracts [ 37 ].…”