Rapid diagnostic tests for malaria

Visser, Theodoor; Daily, Jennifer; Hotte, Nora; Dolkart, Caitlin; Cunningham, Jane; Yadav, Prashant

doi:10.2471/blt.14.151167

Cited by 32 publications

(32 citation statements)

References 25 publications

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“…The hybridization of the target oligo to only its perfectly matched complementary sequence altered the FRET‐pair distance as well as changed the physical topology of the DNA rectangle; these two processes were visualized using fluorescence microscopy and AFM, respectively. Plasmodium falciparum lactate dehydrogenase (PfLDH), a parasite secreted biomarker that is found in elevated amounts in malaria patients, is a key target biomarker for field‐deployable rapid diagnostic tests . PfLDH sensing was similarly demonstrated by capturing the molecule using PfLDH‐specific aptamers on another DNA rectangle and again characterized by AFM .…”

Section: Dna Nanostructures As Biomedical Sensorsmentioning

confidence: 99%

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Mathur

Medintz

2019

Adv Healthcare Materials

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DNA self‐assembly has proven to be a highly versatile tool for engineering complex and dynamic biocompatible nanostructures from the bottom up with a wide range of potential bioapplications currently being pursued. Primary among these is healthcare, with the goal of developing diagnostic, imaging, and drug delivery devices along with combinatorial theranostic devices. The path to understanding a role for DNA nanotechnology in biomedical sciences is being approached carefully and systematically, starting from analyzing the stability and immune‐stimulatory properties of DNA nanostructures in physiological conditions, to estimating their accessibility and application inside cellular and model animal systems. Much remains to be uncovered but the field continues to show promising results toward developing useful biomedical devices. This review discusses some aspects of DNA nanotechnology that makes it a favorable ingredient for creating nanoscale research and biomedical devices and looks at experiments undertaken to determine its stability in vivo. This is presented in conjugation with examples of state‐of‐the‐art developments in biomolecular sensing, imaging, and drug delivery. Finally, some of the major challenges that warrant the attention of the scientific community are highlighted, in order to advance the field into clinically relevant applications.

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Section: Dna Nanostructures As Biomedical Sensorsmentioning

confidence: 99%

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Mathur

Medintz

2019

Adv Healthcare Materials

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“…Populations living in remote areas were frequently neglected. Today RDTs overcome many bottlenecks encountered by microscopy: it is faster to train unskilled personnel to use RDTs than to train a good microscopy technician; there is no ‘fatigue’ or loss of blood smear reading skills because of a rarity of positive cases in a context of decreasing incidence; and, RDTs are easy to supply [ 22 , 23 ]. Recent systematic reviews have concluded that RDT interpretation and performance are satisfactory when handled by trained community members and can result in the appropriate prescription of ACT for confirmed P. falciparum cases [ 24 – 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent improvements in RDT sensitivity and specificity mean that they perform as well as microscopy for symptomatic falciparum malaria infections in many settings [ 27 ]. RDTs can reliably discriminate between P. falciparum and P. vivax infections [ 22 , 28 , 29 ]. The good performances achieved by RDTs should be taken as strong encouragement to further develop this tool.…”

Section: Introductionmentioning

confidence: 99%

The role of early detection and treatment in malaria elimination

Landier

Parker

Thu

et al. 2016

Malar J

119

103

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Falciparum malaria persists in hard-to-reach areas or demographic groups that are missed by conventional healthcare systems but could be reached by trained community members in a malaria post (MP). The main focus of a MP is to provide uninterrupted and rapid access to rapid diagnostic tests (RDTs) and artemisinin-based combination therapy (ACT) too all inhabitants of a village. RDTs allow trained community members to perform malaria diagnosis accurately and prescribe appropriate treatment, reducing as much as possible any delay between the onset of fever and treatment. Early treatment with ACT and with a low-dose of primaquine prevents further transmission from human to mosquito. A functioning MP represents an essential component of any malaria elimination strategy. Implementing large-scale, high-coverage, community-based early diagnosis and treatment through MPs requires few technological innovations but relies on a very well structured organization able to train, supervise and supply MPs, to monitor activity and to perform strict malaria surveillance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-016-1399-y) contains supplementary material, which is available to authorized users.

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“…Molecular biology, however, remains expensive for many countries in endemic areas [16]. Serologic assays constitute a good alternative for epidemiological survey and blood donor screening, given that, antibodies reflect exposure to pathogens [17].…”

Section: Introductionmentioning

confidence: 99%

The hide and seek of Plasmodium vivax in West Africa: report from a large-scale study in Beninese asymptomatic subjects

Poirier

Doderer-Lang

Atchade

et al. 2016

Malar J

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Background Plasmodium vivax is considered to be absent from western Africa, where the prevalence of Duffy-negative red blood cell phenotype proves to be high. Several studies have, however, detected P. vivax infection cases in this part of Africa, raising the question of what is the actual prevalence of P. vivax in local populations. MethodsThe presence of P. vivax was investigated in a large population of healthy blood donors in Benin using microscopy, serology and molecular detection. The seroprevalence was measured with species-specific ELISA using two recombinant P. vivax proteins, namely rPvMSP1 and rPvCSP1. Specific molecular diagnosis of P. vivax infection was carried out using nested-PCR. The performances and cut-off values of both rPvCSP1 and rPvMSP1 ELISA were first assessed using sera from P. vivax-infected patients and from non-exposed subjects.ResultsAmong 1234 Beninese blood donors, no parasites were detected when using microscopy, whereas 28.7% (354/1234) of patients exhibited had antibodies against rPvMSP1, 21.6% (266/1234) against rPvCSP1, and 15.2% (187/1234) against both. Eighty-four samples were selected for nested-PCR analyses, of which 13 were positive for P. vivax nested-PCR and all Duffy negative. ConclusionThe results of the present study highlight an unexpectedly high exposure of Beninese subjects to P. vivax, resulting in sub-microscopic infections. This suggests a probably underestimated and insidious parasite presence in western Africa. While the vaccination campaigns and therapeutic efforts are all focused on Plasmodium falciparum, it is also essential to consider the epidemiological impact of P. vivax.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-016-1620-z) contains supplementary material, which is available to authorized users.

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Rapid diagnostic tests for malaria

Cited by 32 publications

References 25 publications

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

The role of early detection and treatment in malaria elimination

The hide and seek of Plasmodium vivax in West Africa: report from a large-scale study in Beninese asymptomatic subjects

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