Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901

Svastova, Petra; Pavlík, I.; Bartoš, Milan

doi:10.17221/5814-vetmed

Cited by 16 publications

(10 citation statements)

References 11 publications

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“…PCR techniques have dramatically made significant progress in the past ten years and enabled prompt and accurate identification of M. a. paratuberculosis (Svastova et al, 2002). But in the case of the paucibacillary form of paratuberculosis, the low numbers of the microorganisms limit the use of PCR techniques, by which viable and dead bacilli cannot be distinguished.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

Amemori¹,

Mátlová²,

Fischer³

et al. 2004

Vet. Med. - Czech

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ABSTRACT:The gastrointestinal tract (GIT) is a major target for Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) in ca�le. Culture examination was achieved in tissue samples obtained from 10 different regions of the GIT (proximal and distal parts of the duodenum, proximal, middle and distal parts of the jejunum, proximal and distal parts of the ileum, the ileocecal valve, the caecum and the rectum) and their adjacent lymph nodes. The culture results were statistically analysed to elucidate the distribution of M. a. paratuberculosis in the GIT. A total of 63 cows older than 24 months were diagnosed with paratuberculosis by faecal and tissue cultures. The be�er detection rate of M. a. paratuberculosis was found in the mucosae from the jejunum to the ileocecal valve and in the lymph nodes from the jejunum to the caecum. The mean number of colony forming units (CFU) in the mucosae and the lymph nodes of the distal jejunum and the proximal ileum was significantly higher than that in the mucosae of the duodenum, the caecum and the rectum, and in the lymph nodes of the duodenum and the rectum, respectively (P < 0.05). Laparoscopic biopsy a�empted out on 4 animals to test its potential use for sample collection from the statistically optimal mesenteric lymph nodes; but resulted in an abortive a�empt because these targets were encircled by the intestines, the pressure of which complicated the laparoscopic approach.

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Section: Discussionmentioning

confidence: 99%

“…A total of 890 samples were obtained from (Pavlik et al, 2000). The mycobactin J-dependence test and IS900 PCR were performed to confirm paratuberculosis infection when culture results were suspicious (Svastova et al, 2002).…”

Section: Tissue Culture Analysismentioning

confidence: 99%

Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

Amemori¹,

Mátlová²,

Fischer³

et al. 2004

Vet. Med. - Czech

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“…DNA samples extracted using physical method (the simplest way of extract) was carried out according to the [17] with slight modification. The steps of the procedure are described below, the overnight culture pelted by centrifugation13000 rpm to 4 min.…”

Section: Physical Methodsmentioning

confidence: 99%

Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria

Abdelhai¹

2016

BIO

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Abstract:Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the timeconsuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.

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“…The simplest way of DNA release from mycobacterial suspension is boiling for 10 to 15 min in distilled water (Tortoli et al, 2001;Svastova et al, 2002).…”

Section: Physical Methodsmentioning

confidence: 99%

Methods of mycobacterial DNA isolation from different biological material: a review

Hosek¹,

Svastova²,

Morávková³

et al. 2006

Vet. Med.

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Mycobacteria cause serious infections in animals and human beings. Huge economic losses on farms are caused by selected species of this wide family. A high risk of transmission of infection from animal to human exists. The knowledge of exact pathogen characteristics is an important factor which can improve quick and adequate healing. Cultivation and determination of phenotype is still the "gold standard", but has the disadvantage of taking a long time and also low detection limit. Biochemical characterisation of isolates is not exact, and it is expensive. A more popular method used is the amplification of specific loci by polymerase chain reaction (PCR). For this method, the isolation of sufficient amounts of purified DNA is necessary. In this paper the most frequently used method for DNA isolation from live mycobacterial cells, body fluids, tissues, histological samples and forensic materials are outlined. This paper assists only as guide for these methods, so we describe them briefly.Keywords: Johne's disease; Crohn's disease; zoonoses List of abbreviations: BCG = Bacille Calmette-Guérin; CB18 = C18-carboxypropylbetain; CFU = colony forming units; CPH = N-cetylpyridinium chloride, cerebrospinal fluid; CTAB = cetyltrimethyl ammonium bromide; DNA = deoxyribonucleic acid; DR = direct repeat domain; DTT = dithiotreitol; EDTA = disodium ethylene diamine tetraacetate 2 H 2 O; FB = freezing and boiling; HIV/AIDS = Human Immune-Deficiency Virus/Acquired Immune Deficiency Syndrome; IgG = class G immunoglobulin; IMS = immunomagnetic separation; IS = insertion sequence; LCM = laser capture micro-dissection; MAC = Mycobacterium avium complex; MOTT = mycobacteria other than tuberculosis; MTC = Mycobacterium tuberculosis complex; NALC = N-acetyl-L-cystein; OTN-PCR = one-tube nested polymerase chain reaction; PBS = phosphate-buffered saline; PCI = phenol-chloroform-isoamylalcohol; PCR = polymerase chain reaction; PRA-PCR = polymerase restriction analysis-polymerase chain reaction; RFLP = restriction fragment length polymorphism; rRNA = ribosomal ribonucleic acid; SDS = sodium dodecyl sulphate; TE = Tris-HCl + EDTA buffer; TLC = thin-layer chromatography; TSA = tuberculostearic acid: 10-methyloktadekan acid

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Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901

Cited by 16 publications

References 11 publications

Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy

Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria

Methods of mycobacterial DNA isolation from different biological material: a review

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