Rapid discrimination of Porphyra tenera Kjellman var. tamatsuensis Miura by PCR-RFLP

Abstract

To allow to discriminate rapidly the strains of Porphyra tenera var. tamatsuensis, cultivars of which grow more vigorously than strains of P. tenera var. tenera, strains of both varieties were examined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis using mitochondrial DNA related to the ATP synthase F0 subunit 6 (ATP6) gene. The lengths of all sequences in this region of three strains of each variety were 670 bp and had just a single nucleotide substitution. Digestion with the restriction enzyme of TaaI yielded three visible bands which appeared in the three strains of P. tenera var. tamatsuensis, whereas two bands appeared in the three strains of P. tenera var. tenera. We therefore conclude that PCR-RFLP analysis is a valuable tool for discrimination of P. tenera var. tamatsuensis among the stock of P. tenera strains used for mariculture.

Prospects and challenges for industrial production of seaweed bioactives

“…Altered bioactive profiles can result from changes in anti-grazing defenses (e.g., polyphenol levels) as modulated by grazing intensity, and increased antioxidant capacity has been reported in response to infection (e.g., Caulerpa taxifolia; Box et al 2008). Several reports on the molecular genetic diversity of commercial genera such Pyropia, Kappaphycus and Eucheuma, highlight the fact that selection based on morphology and product quality can be supplemented by molecular techniques (Park et al 2008, Abe et al 2010, Chan et al 2012a). The documented genetic diversity of wild and cultivated strains or ecotypes available for aquaculture (Zuccarello et al 2007) is currently not being fully exploited (Halling et al 2013).…”

Development of quality control and evaluation techniques for dried sheets of Asakusa-nori: Simple estimation of <i>Pyropia tenera</i> thallus content in dried sheets using PCR analysis

“…In panel (c), the relative ‰uorescence intensities of the PtMIE orf169 trnV mtDNA fragments to the PyU51 orf169 trnV mtDNA fragments were derived from the exponential phase of ampliˆcation (18 22 cycles) in panel (a), and the data were plotted as a function of the relative ‰uorescence intensity versus total DNA content of PtMIE. 6,7,12)…”