Rapid Dissolution of ZnO Nanoparticles Induced by Biological Buffers Significantly Impacts Cytotoxicity

Eixenberger, Josh; Anders, Catherine B.; Hermann, Rebecca; Brown, Raquel J.; Reddy, K. M.; Punnoose, Alex; Wingett, Denise

doi:10.1021/acs.chemrestox.7b00136

Cited by 57 publications

(29 citation statements)

References 59 publications

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“…However, a similar, but non-statistically significant increase in neutrophil influx from 0.5% to 3.9% was seen for the uncoated ZnO nanoparticle on day 3 postexposure. The observed differences in inflammatory response could be due to the differential solubility of the two ZnO types and the high level of protein in BAL from the ZnO-exposed mice could be a result of dissolution-mediated cytotoxicity (M€ uller et al 2010;Eixenberger et al 2017). ZnO nanoparticles of different sizes have been reported to induce neutrophil influx in BAL fluid in rat and mouse models following inhalation exposure to mass concentrations of 1 to 12 mg/m 3 of ZnO nanoparticles (Conner et al 1988;Ho et al 2011;Adamcakova-Dodd et al 2014;Chuang et al 2014;Chen et al 2015;Larsen et al 2016).…”

Section: Effects On Pulmonary Inflammationmentioning

confidence: 99%

“…The coated ZnO may dissolve slower than the uncoated ZnO nanoparticle, which is reflected by the increased differential gene expression on day 28. ZnO nanoparticles have been shown to quickly dissolve in biological tissues (Xia et al 2008;Adam et al 2014;Eixenberger et al 2017). The dissolution behavior of the uncoated ZnO (NM-110) and the coated ZnO (NM-111) in water and cell culture medium have been previously studied; at the 1 mg/mL concentration, 60% of the uncoated ZnO dissolves in water at 24 h vs. 18% for the coated ZnO nanoparticle.…”

Section: Differences In Toxicity Of the Coated And Uncoated Zno Nanopmentioning

confidence: 99%

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Acute phase response and inflammation following pulmonary exposure to low doses of zinc oxide nanoparticles in mice

et al. 2019

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Inhalation of nanosized zinc oxide (ZnO) induces metal fume fever and systemic acute phase response in humans. Acute phase response activation is a cardiovascular risk factor; we investigated whether pulmonary exposure of mice can be used to assess ZnO-induced acute phase response as well as inflammation and genotoxicity. Uncoated (NM-110) and triethoxycaprylylsilane-coated (NM-111) ZnO nanoparticles were intratracheally instilled once at 0.2, 0.7 or 2 mg/ mouse (11, 33 and 100 mg/kg body weight). Serum amyloid A3 mRNA level in lung tissue, bronchoalveolar lavage (BAL) fluid cellularity, and levels of DNA strand breaks in BAL fluid cells, lung and liver tissue were assessed 1, 3 and 28 days post-exposure. Global transcription patterns were assessed in lung tissue using microarrays. The acute-phase response serum amyloid A3 mRNA levels were increased on day 1; for uncoated ZnO nanoparticles at the highest dose and for coated ZnO nanoparticles at medium and highest dose. Neutrophils were increased in BAL fluid only after exposure to coated ZnO nanoparticles. Genotoxicity was observed only in single dose groups, with no dose-response relationship. Most changes in global transcriptional response were observed after exposure to uncoated ZnO nanoparticles and involved cell cycle G2 to M phase DNA damage checkpoint regulation. Although, uncoated and coated ZnO nanoparticles qualitatively exerted similar effects, observed differences are likely explained by differences in solubility kinetics. The finding of serum amyloid A3 induction at low exposure suggests that mouse models can be used to assess the nanoparticle-mediated induction of acute phase responses in humans.

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Section: Effects On Pulmonary Inflammationmentioning

confidence: 99%

Section: Differences In Toxicity Of the Coated And Uncoated Zno Nanopmentioning

confidence: 99%

Acute phase response and inflammation following pulmonary exposure to low doses of zinc oxide nanoparticles in mice

et al. 2019

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“…However, when cell toxicity develops, it is probably due to both NPs as such and to the ions released by dissolution in the culture medium or after entering the cell. It is recognized that dissolution is one of the main sources of NP toxicity, and although it can take considerable time to perform such a study and it is complicated by issues such as particle separation from solution, it represents a key aspect of nanotoxicology [30]. Co ions are involved in several cellular processes, for example, in the induction of hypoxia by the increase in the inducible HIF-1α, which, in turn, could be the cause of reduced cell proliferation [31].…”

Section: Discussionmentioning

confidence: 99%

Recovery Ability of Human Adipose Stem Cells Exposed to Cobalt Nanoparticles: Outcome of Dissolution

Borgese

Rossi

Bonfanti

et al. 2020

Nanomedicine (Lond.)

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Aim:To demonstrate that cobalt nanoparticles doses are safe for use in humans and to understand the consequences of the particulate effects, which may persist inside the cells. Materials & methods: Human adipose stem cells were used. We evaluated cell recovery by viability test, morphology and ultrastructure using electronic and optical microscopy, while gene expression was assessed utilizing real-time PCR. Results: After exposure, most stem cells recovered their normal function. Co 3 O 4 -nanoparticles remained inside the cell for the entirety of the considered time. A slight modification of gene expression was observed in the exposed cells. Conclusion: After exposure to 100 M cobalt nanoparticles, most cells returned to normal function. Nanoparticle toxicity was due to ions released by dissolution as well as from the nanoparticles themselves.Cobalt (Co), along with iron (Fe) and nickel (Ni), is a transition metal belonging to group VIIIb of the periodic table. It is an essential element for cell physiology and animal health; in fact, Co is an important constituent of vitamin B12 and is involved in several cell pathways, such as the metabolism of fats and carbohydrates, the conversion of folate into their active form, protein synthesis and the prevention of demyelination [1]. Co deficiency is quite unusual and potentially lethal; conversely, Co intoxication, caused by excessive levels in the body, occurs more frequently and is dangerous as well [2].Along with Fe and Ni, Co is one of the three naturally occurring magnetic metals; it is able to retain its magnetic properties at high temperatures. Therefore, Co has many applications, such as for energy storage systems and catalytic processes [3]. In particular, Co, in its bulk form, is used for green technologies and is also an integral component in powering electric vehicles, wind and wave generators, solar energy technologies sensors, electrochemistry and magnetic fluids [4,5]. However, at the nanoscale dimension, too, Co is promising for several nanotechnological applications ranging from chemistry to nanomedicine, where it is used as a highly sensitive MRI contrast agent [6]. In this scenario, an issue that should be considered is the exponential growth of production, use and discharge of Co nanoparticles (Co-NPs). Consequently, the human body is very likely to come into contact, intentionally or not, with Co-NPs that may easily enter the body in different ways [7][8][9], encounter cells of different tissues and then act on them by perturbing their physiology [10]. In this perspective, special attention should be paid to the presence of multipotent stem cells, a population residing in almost all human tissues that fulfills important functions, including renewal, repair and remodeling [11]. Among the different stem cell populations, mesenchymal stem cells (MSCs) are very interesting due to their great potential in many medical applications, such as regenerative medicine, bone marrow transplantation and orthopedic injuries. Unfortunately, MSCs are more sensitive ...

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“…Porous zinc oxide nanoparticles were synthesized using the forced hydrolysis method (Eixenberger et al, 2017). Zinc acetate dihydrate (1.000 g) was added to diethylene glycol (DEG), (100 mL), and the solution was brought to 85 ˚C.…”

Section: Synthesis and Characterization Of Pzno Npsmentioning

confidence: 99%

Synthesis of cisplatin encapsulated Zinc oxide nanoparticles and their application as a carrier in targeted drug delivery

2020

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Cisplatin is a frequently used anticancer drug that has been developed as the first platinum-based anticancer drug. The cis configuration enables the coordination complex to be covalently binding to one or two DNA strands and thus crosslinking the DNA strands, causing the cells to die in a programmed manner. Cisplatin is administered as an IV infusion in saline solution for medication of solid malignity. Anticancer drugs usually have a variety of side effects, but an encapsulation of the drug in a suitable host material minimizes the side effects while improving the efficacy of the drug due to its slow release only at the target. The aim of this research is to develop a simple, but effective mechanism for the preparation of porous zinc oxide nanoparticles (PZnO NPs) using the forced hydrolysis method reaction of zinc acetate dihydrate with deionized water in diethylene glycol (DEG) media. This synthesized PZnO NPs were then characterized by Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDX), Fourier Transform Infrared Spectroscopy (FT-IR), Particle Size Analysis and Powder X-Ray Diffraction (PXRD). The encapsulation of cisplatin within the porous zinc oxide nanoparticles was confirmed by X-ray Fluorescence (XRF), SEM, EDX, and FT-IR studies. Our results show that the synthesized nanoparticles have the hexagonal wurtzite structure as confirmed by PXRD. The average particle size as determined by light scattering is 52.4 ± 0.1 nm SEM images show porous spherical morphology with aggregated particles. XRF data of the cisplatin encapsulated product show a Pt: Cl ratio of 1:2 showing cisplatin encapsulation without any fragmentation or other chemical change. The presence of NH 3 in the encapsulated product is also apparent from FT-IR data. The encapsulation of the anti-cancer drug cisplatin to PZnO NPs and its pH dependence on the release of the drug from PZnO NPs was studied by measuring the amount of Pt released as a function of the time which was done using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) at λ max 265.94 nm. The encapsulation efficiency of Cisplatin into PZnO NPs was found to be 50.52%. The percentage of Cisplatin released from PZnO NPs during the first 7 hours was < 6.30% in the acetate/ phosphate buffer at pH 4.0, 5.0, 6.0, 7.0 and 8.0. The maximum release of 8.64% was observed at pH = 6.0 after 24 hours.

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Rapid Dissolution of ZnO Nanoparticles Induced by Biological Buffers Significantly Impacts Cytotoxicity

Cited by 57 publications

References 59 publications

Acute phase response and inflammation following pulmonary exposure to low doses of zinc oxide nanoparticles in mice

Acute phase response and inflammation following pulmonary exposure to low doses of zinc oxide nanoparticles in mice

Recovery Ability of Human Adipose Stem Cells Exposed to Cobalt Nanoparticles: Outcome of Dissolution

Synthesis of cisplatin encapsulated Zinc oxide nanoparticles and their application as a carrier in targeted drug delivery

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