Rapid DNA fingerprinting using alkaline phosphatase-conjugated oligonucleotides

Edman, Jeffrey C.; Evans-Holm, Martha; Marich, Jim E.; Ruth, Jerry L.

doi:10.1093/nar/16.13.6235

Cited by 20 publications

(4 citation statements)

References 2 publications

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“…After electrophoresis, the separated DNA was transferred for three hours by southern blotting to Nylon membranes, rinsed, then vacuum dried overnight. The membrane-bound DNA was hybridized (using a Robbins hybridization incubator) to an oligonucleotide probe, conjugated with alkaline phosphatase (Molecular Biosystems, Inc.), corresponding (EDMAN et al, 1988) to the core sequence of a clone (33.6 minisatellite) of the human myoglobin gene (JEvFREYS et al, 1985). Bands were visualized by incubating the membrane for several hours in a Tris-HCl solution containing 80/xl each of NBT (75 mg/ml) and BCIP (50 mg/ml).…”

Section: Methodsmentioning

confidence: 99%

A 15-year study of the association between dominance rank and reproductive success of male rhesus macaques

Smith

1993

Primates

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ABSTRACT. The reproductive success (RS) of 32 males in a captive group of rhesus macaques (Macaca mulatta) between 1978 and 1992 was determined using paternity exclusion analysis. Dominance rank of each male over age 4 was assessed at the end of each breeding season based on agonistic dyadic interactions. The dominance rank and RS of these males were strongly correlated whether or not subadult males were included. The high reproductive success of males that eventually reached alpha rank is primarily responsible for this outcome. These results support the theory that social dominance has evolved in genus Macaca by sexual selection but some changes in male dominance rank and RS during the 15-year period suggest that priority of access is not the sole focus for such selection.

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Section: Methodsmentioning

confidence: 99%

A 15-year study of the association between dominance rank and reproductive success of male rhesus macaques

Smith

1993

Primates

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“…7 In each experiment, the DNA samples from rats from different sources were processed simultaneously and run on a single gel. After overnight transfer to a nylon membrane, ultraviolet fixation, and a 15-minute prehybridization, the DNA was hybridized for 20 minutes at 50° C with an alkaline phosphataseconjugated oligonucleotide, TGG AGG AGG GCT GGA GGA GGG C (Molecular Biosystems Inc., San Diego, California), 8 corresponding to the core sequence of the human myoglobin 33.6 minisatellite described by Jeffreys et al 9 This probe detects multiple loci scattered throughout the mammalian genome that contain tandem repeats of similar DNA sequence. 8 Because there is extensive variability in the number of tandem repeats at each of these loci, the probe can reveal different DNA restriction fragment patterns (i.e., different DNA fingerprints) in genetically different individuals.…”

Section: Dna Fingerprint Analysismentioning

confidence: 99%

Molecular evidence of genetic heterogeneity in Wistar-Kyoto rats: implications for research with the spontaneously hypertensive rat.

et al. 1989

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Spontaneously hypertensive rats (SHR) and nonnotensive Wistar-Kyoto ("WKY") rats are frequently employed in experimental studies of hypertension. Although both SHR and WKY rat strains have been presumed to be fully inbred, recent studies have revealed important biologic variability in WKY rats from different commercial sources. Genealogk evidence suggests that, in the United States, breeding stocks of WKY rats may have been distributed to major commercial suppliers as early as the F10 generation. To test the hypothesis that commercially available WKY rats are genetically heterogeneous, we performed deoxyribonucleic acid (DNA) "fingerprint" analysis on genomic DNA of WKY rats from two of the largest vendors in the United States, Taconic Farms and Charles River Laboratories. We found molecular evidence of genetic variability not only among WKY rats from two different breeding facilities, but also among WKY rats within a single breeding facility (Taconic Farms). Although some studies have suggested the possibility of biologic variability in SHR from different sources, preliminary studies have not revealed molecular evidence of genetic heterogeneity in SHR from these vendors. In demonstrating genetic variability in WKY rats from different sources, the current study provides compelling evidence that rats designated WKY do not constitute an inbred strain. Accordingly, the results of studies in which SHR and WKY rats are compared might vary because of genetic heterogeneity in "the WKY rat control strain." (Hypertension 1989; 13:188-192) T he spontaneously hypertensive rat (SHR), initially bred in Kyoto, Japan, is the most widely studied experimental model of human essential hypertension. 12 Over the past 2 decades, numerous investigators have studied the pathogenesis of spontaneous hypertension by comparing SHR with normotensive Wistar-Kyoto rats (the so-called "WKY" strain) with respect to an enormous variety of physiological and biochemical characteristics. 2 Although both SHR and WKY rat strains have been presumed to be fully inbred, 3 recent studies have revealed important biologic

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“…We initially hybridized membranes with two common VNTR-homologous oligonucleotides, one based on Jeffreys 33.6 core fragment (Edman et a!., 1988) and the other homologous to bacteriophage M13 (Vassart et a!., 1987), which detect DNA fingerprints in salmonids (Spruell et a!., 1994). Although fewer bands were detected in the other species than are observed in salmonids, some sharp bands were observed in each species.…”

Section: Methodsmentioning

confidence: 99%

SINE sequences detect DNA fingerprints in salmonid fishes

Spruell

Thorgaard

1996

Heredity

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DNA probes homologous to two previously described salmonid short interspersed nuclear elements (SINEs) detected DNA fingerprint patterns in 14 species of salmonid fishes. The probes showed more homology to some species than to others and little homology to three nonsalmonid fishes. The DNA fingerprint patterns derived from the SINE probes are individual-specific and inherited in a Mendelian manner. Probes derived from different regions of the same SINE detect only partially overlapping banding patterns, reflecting a more complex SINE structure than has been previously reported. Like the human Mu sequence, the SINEs found in salmonids could provide useful genetic markers and primer sites for PCR-based techniques. These elements may be more desirable for some applications than traditional DNA fingerprinting probes that detect tandemly repeated arrays.

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Rapid DNA fingerprinting using alkaline phosphatase-conjugated oligonucleotides

Cited by 20 publications

References 2 publications

A 15-year study of the association between dominance rank and reproductive success of male rhesus macaques

A 15-year study of the association between dominance rank and reproductive success of male rhesus macaques

Molecular evidence of genetic heterogeneity in Wistar-Kyoto rats: implications for research with the spontaneously hypertensive rat.

SINE sequences detect DNA fingerprints in salmonid fishes

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