Rapid DNA sequencing based upon single molecule detection

Davis, Lloyd M.; Fairfield, Frederic R.; Harger, Carol; Jett, James H.; Keller, Richard A.; Hahn, Jong Hoon; Krakowski, Letitia A.; Marrone, Babetta L.; Martin, John; Nutter, Harvey L.; Ratliff, Robert L.; Shera, E.B.; Simpson, Daniel J.; Soper, Steven A.

doi:10.1016/1050-3862(91)90002-9

Cited by 62 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Others have sought to develop sequencing methods that rely on detecting single molecules (e.g., via exonuclease digestion [72,[85][86][87][88] or by detecting the nature or existence of PCR products ampliWed therefrom [67][68][69][70][71]); recently, the incorporation of nucleotides has been observed on single DNA strands by Xuorescence microscopy [89]. Future progress depends on the ability to observe primer extension with ever lower nucleotide concentrations.…”

Section: Discussionmentioning

confidence: 99%

Single-nucleotide polymorphism detection using nanomolar nucleotides and single-molecule fluorescence

Twist

Winson

Rowland

et al. 2004

Analytical Biochemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Single-nucleotide polymorphism detection using nanomolar nucleotides and single-molecule fluorescence

Twist

Winson

Rowland

et al. 2004

Analytical Biochemistry

View full text Add to dashboard Cite

“…This feature is ideal for observing molecular motion in two dimensions, and is needed for newly suggested methods for rapid DNA sequencing, which involve imaging individual dye molecules nearby an array of nanofabricated features [9,10]. Such applications have become even more feasible with recent improvements in the quantum efficiency of the CCD.…”

Section: Single-molecule Imaging Applicationsmentioning

confidence: 99%

“…One such scheme involves the cleaving of a fluorescent tag from a DNA base as it becomes incorporated into the double helix [9]. Another plan is the opposite of this; each fluorescently labeled base is sequentially removed from a single DNA strand, and then flows through the laser beam for detection [10].…”

Section: Single-molecule Imaging Applicationsmentioning

confidence: 99%

Single-Molecule Imaging

Gargas¹,

Chan²,

Ostrowski³

et al. 2013

Encyclopedia of Biophysics

View full text Add to dashboard Cite

“…A variety of novel approaches based on single molecule analysis are being investigated to provide revolutionary approaches for DNA sequencing and analysis (Davis et al 1991;Chen and Dovichi 1996;Fernandes 1998;Haab and Mathies 1998;Nie and Zare 1998;Rich 1998;Akeson et al 1999;Herrick and Bensimon 1999;Ishikawa 1999;Moore et al 1999;Yeung 1999;Bustamante et al 2000;Deamer and Akeson 2000;Jett et al 2000;Krylov and Dovichi 2000;Van Craenenbroeck and Engelborghs 2000). One example is the research by Dr. Deamer at the University of California, Santa Cruz and Dr. Branton at Harvard University to develop single molecule electrophoresis (Akeson et al 1999;Akeson and Deamer 1999;Deamer and Akeson 2000;Service 2000).…”

Section: Single Molecule Analysismentioning

confidence: 99%

“…Again, this approach may yield a very rapid, low cost sequencing method. See also Jett et al (1989) and Davis et al (1991).…”

Section: Single Molecule Analysismentioning

confidence: 99%

Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends

Meldrum¹

2000

Genome Res.

116

View full text Add to dashboard Cite

Automation for genomics has enabled a 43-fold increase in the total finished human genomic sequence in the world in the past four years. This is the second half of a two-part, noncomprehensive review that presents an overview of different types of automation equipment used in genome sequencing. The first part of the review, published in the previous issue, focused on automated procedures used to prepare DNA for sequencing or analysis. This second part of the review presents a look at available DNA sequencers and array technology and concludes with a look at future technologies. Alternate sequencing technologies including mass spectrometry, biochips, and single molecule analysis are included in this review.Sequencing data, essential for many of the medical breakthroughs of today and tomorrow, is currently accumulating at an exponential rate, largely because of advances in sequencing methods and laboratory automation. Instruments are available that can automate nearly every step in the large-scale sequencing process.The first article in this two-part review (Meldrum 2000) presented a description of automation designed for isolating DNA, cloning or amplifying DNA, preparing enzymatic sequencing reactions, and purifying DNA. Part one of the review also showed that the majority of genome centers use both in-house automation and commercial automation (Collins et al. 1998;Wendl et al. 1998;Mullikin and McMurragy 1999;Spurr et al. 1999; see http://www.genome.org for a supplementary table providing a snapshot view of automation used at a number of different genome centers).In this, the second part of the review, the focus is on automation after DNA preparation and sequencing reactions-on obtaining the sequence and its analysis. A discussion of future technologies for DNA sequencing projects is also included. (See the web sites referenced for photos or further details on the instruments presented.)

show abstract

Rapid DNA sequencing based upon single molecule detection

Cited by 62 publications

References 15 publications

Single-nucleotide polymorphism detection using nanomolar nucleotides and single-molecule fluorescence

Single-nucleotide polymorphism detection using nanomolar nucleotides and single-molecule fluorescence

Single-Molecule Imaging

Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends

Contact Info

Product

Resources

About