Rapid duplex immunoassay for wound biomarkers at the point-of-care

Worsley, Graham J.; Attree, Simon; Noble, James; Horgan, Adrian

doi:10.1016/j.bios.2012.02.005

Cited by 44 publications

(52 citation statements)

References 19 publications

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“…Fluorescent microspheres (FMs), which have a stable configuration and a high fluorescence intensity, are multicolored and safe. Due to the lack of sensitivity when applying AuNPs, FMs are used mainly for the detection of several compounds, such as aflatoxin M1 (AFM1) (98), tumor necrosis factor alpha (TNFα) [200], procalcitonin [201], 4(5)-methylimidazole [202], E.coli [203]. concentrations caused more interaction between E.coli and anti-E.coli antibody.…”

Section: Other Fluorescent Materialsmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

503

315

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Section: Other Fluorescent Materialsmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

503

315

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“…Worsley et al described a lateral flow assay for IL-6 and TNF-α that was able to detect these two important cytokines into the 7–10 pg/mL range in plasma [115]. Using up-converting phosphor technology, IL-10 was detected at a targeted level of 100 pg/mL [116].…”

Section: Antibody-based Flow Assaysmentioning

confidence: 99%

Bioanalytical chemistry of cytokines – A review

Stenken

Poschenrieder

2015

Analytica Chimica Acta

245

172

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Cytokines are bioactive proteins produced by many different cells of the immune system. Due to their role in different inflammatory disease states and maintaining homeostasis, there is enormous clinical interest in the quantitation of cytokines. The typical standard methods for quantitation of cytokines are immunoassay-based techniques including enzyme-linked immusorbent assays (ELISA) and bead-based immunoassays read by either standard or modified flow cytometers. A review of recent developments in analytical methods for measurements of cytokine proteins is provided. This review briefly covers cytokine biology and the analysis challenges associated with measurement of these biomarker proteins for understanding both health and disease. New techniques applied to immunoassay-based assays are presented along with the uses of aptamers, electrochemistry, mass spectrometry, optical resonator-based methods. Methods used for elucidating the release of cytokines from single cells as well as in vivo collection methods are described.

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“…They include assays that use quantum dots (23 ), photoluminescent nanocrystals (24 ), europium (III) chelate-dyed polystyrene nanoparticles (25 ), upconverting phosphors (26 ), microspheres (27 ), silica nanoparticles covalently labeled with lathanide chelates (28 ), dye-filled latex beads (29 ), and dyeconjugated antibodies (3, 4 ). The NIR-LFA, however, has distinct advantages on several critical points.…”

Section: Discussionmentioning

confidence: 99%

“…The NIR-LFA, however, has distinct advantages on several critical points. First, whereas the fluorescent assays reported have emission/ excitation spectrums ranging from UV to 720 nm, with most in the 500-to 600-nm range (3,4,(23)(24)(25)(27)(28)(29), the NIR-LFA uses dye with excitation/emission peaks of 785/820 nm. Use of a dye in the NIR region helps increase the signal-to-noise ratio in plasma and blood by reducing the background from autofluorescent proteins and matrix materials and avoids possible dampening mediated by hemoglobin absorption.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, Corstjens et al (26 ) developed a multiplex LFA that used upconverting phosphors, which measured IL-10 and interferon-␥ in assay buffer at concentrations from 1 to 100 pg/mL. Although both assays achieved high analytical sensitivity, they relied on conjugate-sample premixing, addition of a wash buffer, and in the case of IL-10/ interferon-␥, separate mixing and addition of the different detection moieties to the test strip (26,27 ).…”

Section: Discussionmentioning

confidence: 99%

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Lateral Flow Assay with Near-Infrared Dye for Multiplex Detection

Swanson

D’Andrea

2013

Clinical Chemistry

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BACKGROUND Lateral flow assays (LFAs) are popular point-of-care diagnostic tools because they are rapid and easy to use. Nevertheless, they often lack analytical sensitivity and quantitative output and may be difficult to multiplex, limiting their usefulness in biomarker measurement. As a proof-of-concept study, we detail the design of a quantitative, multiplex LFA with readily available near-infrared (NIR) detection to improve analytical sensitivity. METHODS NIR dye was conjugated to selected antibodies and incorporated into LFAs. We used singleplex, optimized NIR-LFAs to measure interleukin (IL)-6 from 0 to 200 pg/mL and developed duplex assays to simultaneously measure IL-6 from 0 to 100 pg/mL (0 to 4.5 pmol/L) and C-reactive protein (CRP) from 50 to 2500 ng/mL (0.4 to 20 nmol/L) on a single test strip. Assays were tested on 60 different spiked samples and compared to ELISA results. RESULTS NIR-LFAs detected IL-6 in a 10% plasma matrix with a limit of detection of 4 pg/mL (182 fmol/L) and a CV <7%. Duplex NIR-LFAs quantitatively measured IL-6 and CRP concentrations simultaneously. Values strongly correlated to ELISA measurements, with R2 values of 0.9825 and 0.9711 for IL-6 and CRP, respectively. CONCLUSIONS NIR-LFAs exhibit quantitative measurement at pg/mL concentrations owing to a high signal-to-BACKGROUND ratio and robust detection antibody clearance through the test strip. Moreover, NIR-LFAs are able to detect molecules present at vastly different concentrations in multiplex format and compare favorably to ELISAs. LFAs with direct NIR detection may be a valuable tool for biomarker evaluation in the point-of-care setting.

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Rapid duplex immunoassay for wound biomarkers at the point-of-care

Cited by 44 publications

References 19 publications

Lateral flow assays: Principles, designs and labels

Lateral flow assays: Principles, designs and labels

Bioanalytical chemistry of cytokines – A review

Lateral Flow Assay with Near-Infrared Dye for Multiplex Detection

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