Rapid effects of 17β-estradiol and progesterone on sheep visceral and parietal pleurae via a nitric oxide pathway

Abstract

We investigated the effects of 17beta-estradiol and progesterone on transepithelial electrical resistance (R(TE)) in sheep visceral and parietal pleurae. Specimens of intact pleurae from adult female sheep were used. The samples were transferred to the laboratory within 30 min after death of the animal in a Krebs-Ringer solution at 4 degrees C. The pleura was then mounted as a planar sheet in Ussing-type chambers, and electrical measurements were made. There was an increase in R(TE) in all of the samples examined after addition of 17beta-estradiol and progesterone in visceral and parietal pleurae. This increase was rapid within 1 min, lasted for ~15 min, returned to the basal level within 30-45 min, and was dose dependent. Tamoxifen, an estrogen receptor antagonist, did not significantly eliminate the effect of 17beta-estradiol. Furthermore, no steroid receptors were identified in cytosolic preparations of visceral and parietal pleura with ligand binding assays. The estrogen- and progesterone-induced increase in R(TE) in both visceral and parietal pleurae was affected by addition of an inhibitor of nitric oxide synthase. Indeed, previous administration of N(omega)-nitro-L-arginine methyl ester prevented the increase in R(TE) by 17beta-estradiol and progesterone. These results suggest that 17beta-estradiol and progesterone induce an increase in R(TE) in both visceral and parietal pleura and thus alter the transepithelial permeability. The effect of steroids may be accounted for by rapid release of nitric oxide in pleura.

Low glucose level and low pH alter the electrochemical function of human parietal pleura

“…PDTM and RTM were decreased, suggesting that the electrochemical transport ability of the pleura had increased [9]. The RTM decrease was quicker and more intense as less glucose and lower-pH KRB were used.…”

“…Experiments were also conducted with KRB solutions without glucose, balanced at pH 7.2 (n59 experiments). The chamber facing the outer-pleural surface was filled with 5.55 mM glucose KRB balanced at pH 7.45, ensuring tissue viability [9,10]. PDTM was measured 1, 5, 10, 30, 45 and 60 min after the addition of each solution.…”

Lymphangioleiomyomatosis

“…Another issue, not as significant as the above but still important, is the fact that hormonal therapy may be implicated in the occurrence of chylothorax, since it has been speculated that steroids may increase fluid accumulation and prevent the transport of fluid out of the pleural space [10]. …”

Η Επίδραση Αντιοξειδωτικών Παραγόντων Στη Διαπερατότητα Του Υπεζωκότα Προβάτου

“…Παρόλα αυτά, το υπεζωκοτικό υγρό είναι πιο αλκαλικό από το πλάσμα και αυτή η διαφορά είναι δύσκολο να διατηρηθεί αν το μεσοθήλιο είναι εξαιρετικά διαπερατό. Από την άλλη πλευρά, μελέτες έχουν δείξει μείωση της διαπερατότητας τόσο του τοιχωματικού όσο και του περισπλάχνιου υπεζωκότα προβάτου, μετά την προσθήκη ουσιών που εμποδίζουν τη διακίνηση ιόντων αλλά και ορμονών όπως η προγεστερόνη και η οιστραδιόλη [35,36].…”