Rapid electrochemical assay for theophylline in whole blood based on the inhibition of bovine liver alkaline phosphatase

Selective and Sensitive Biosensor for Theophylline Based on Xanthine Oxidase Electrode

“…The level of oxypurines (xanthine and hypoxanthine) in biological samples (blood, plasma) is much lower than the therapeutic level of theophylline (22) and their interference is not expected, but theophylline metabolites, in particular 1-methylxanthine and 3-methylxanthine, could cause a serious interference (16). So the need to improve the biosensor selectivity toward theophylline remains a challenge.…”

“…On the other hand the theophylline determination based on its selective recognition by molecular imprinted polymers is time consuming (14,15). An electrochemical assay based on the inhibition of alkaline phosphatase by theophylline was also developed (16). Gupta et al (17) have described an optical enzymic approach utilizing the theophylline oxidation with theophylline oxidase in the presence of ferricytochrome c, where ferrocytochrome c produced was monitored.…”

Electrochemical Biosensors for Drug Analysis

“…Theophylline is an uncompetitive inhibitor of alkaline phosphatase. The enzyme was solubilized in a carbon suspension and adsorbed on the surface of a disposable screen printed test strip for determining theophylline concentration in whole blood based on the generation of p-aminophenol from p-aminophenyl phosphate by the action of bovine liver alkaline phosphatase [103]. GCE/SiSG/ AuNPs/CHIT/ AChE Galantamine CV - [29] (continued) The coupling of this enzyme with tyrosinase (monophenolmonoxygenase) or polyphenol oxidase (PPO) to monitor oxygen consumption or quinone reduction has been proposed for salicylate detection [109][110][111].…”

Eletrodos fabricados por "silk-screen"

“…A mesma formulação foi também usada em suporte cerâmico de alumina por outros autores 21,30,31 . Formulações à base de água como solvente têm sido também empregadas 4,29,32 , inclusive para explorar a atividade catalítica de pó de grafite metalizado com Pt, Ni, Cr, Rh, Pd, Ru e Ag 4 num estudo que combina tecnologias de materiais catalíticos, procedimentos de imobilização de enzimas, deposição de membranas, "screen-printing" e jato de tinta na construção de biossensores. Nesse trabalho 4 , pó de grafite metalizado, polímero hidroxietilcelulose e solução tampão fosfato pH 7 são misturados para formar a tinta.…”