Rapid electrochemical detection of Escherichia coli using nickel oxidation reaction on a rotating disk electrode

Ramanujam, A.; Neyhouse, Bertrand J.; Keogh, Rebecca A.; Muthuvel, Madhivanan; O’Carroll, Ronan; Botte, Gerardine G.

doi:10.1016/j.cej.2021.128453

Cited by 36 publications

(22 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This ultra-fast platform technology could be expanded for the continuous monitoring of SARS-CoV-2 in air, water, wastewater, etc. In the past, a similar sensor has been used for detecting the bacterium E. coli in water and wastewater [29].…”

Section: Discussionmentioning

confidence: 99%

“…The standard operating procedure for each sample consisted of three steps, namely cyclic voltammetry (activation), chronoamperometry (testing) and the rinsing step. A similar approach was previously used to detect the bacterium E. coli in water and wastewater [29]. The electrochemical measurements were performed using a Gamry Framework TM Processes 2021, 9, 1236 4 of 13 data acquisition software version 7.8.2 associated with Gamry Reference 600+ Potentiostat (Gamry Instruments, Warminster, PA, USA).…”

Section: Testing Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

Ultra-Fast Electrochemical Sensor for Point-of-Care COVID-19 Diagnosis Using Non-Invasive Saliva Sampling

2021

Self Cite

View full text Add to dashboard Cite

Point-of-care diagnostic devices that are rapid and reliable remain as an unmet need highlighted by the coronavirus disease (COVID-19) pandemic crisis. The second/third wave of virus spread in various parts of the world combined with new evidence of re-infections and inadequate healthcare facilities demand increased testing rate to diagnose COVID-19 at its core. Although traditional molecular diagnostic tests have served this purpose, there have been shortage of reagents and other supplies at pandemic frontlines. This calls for novel alternate diagnostic processes with potential for obtaining emergency use authorization and that can be deployed in the field at the earliest opportunity. Here, we show an ultra-fast SARS-CoV-2 detection sensor for detecting coronavirus proteins in saliva within 100 milliseconds. Electrochemical oxidation of nickel hydroxide has been controlled using cyclic voltammetry and chronoamperometry techniques for successful detection of SARS-CoV-2. Test results have proven the capability of sensors to quantitatively detect the concentration of virus in blinded analyses. The detection occurs by a process similar to that of SARS-CoV-2 binding onto host cells. The sensor also shows prospects in distinguishing SARS-CoV-2 from other viruses such as HIV. More importantly, the sensor matches the detection limit of the gold standard test for diagnosing early infection. The use of saliva as a non-invasive sampling technique combined with the portability of the instrument has broadened the potential of this sensor.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Testing Proceduresmentioning

confidence: 99%

Ultra-Fast Electrochemical Sensor for Point-of-Care COVID-19 Diagnosis Using Non-Invasive Saliva Sampling

2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nickel is a versatile metal that is used in several products, from simple day-by-day coinage [246] to electrodes used for Escherichia coli detection in water [247]. It is one of the metals contained in stainless steel [248], a stable alloy used to construct several industrial products.…”

Section: Nickel-catalyzed C-h Activationmentioning

confidence: 99%

On the application of 3d metals for C–H activation toward bioactive compounds: The key step for the synthesis of silver bullets

Carvalho¹,

Miranda²,

Nunes³

et al. 2021

Beilstein J. Org. Chem.

View full text Add to dashboard Cite

Several valuable biologically active molecules can be obtained through C–H activation processes. However, the use of expensive and not readily accessible catalysts complicates the process of pharmacological application of these compounds. A plausible way to overcome this issue is developing and using cheaper, more accessible, and equally effective catalysts. First-row transition (3d) metals have shown to be important catalysts in this matter. This review summarizes the use of 3d metal catalysts in C–H activation processes to obtain potentially (or proved) biologically active compounds.

show abstract

“…E. coli causes diarrhoea, urinary infections, and peritonitis, predominantly in vulnerable people, and it is often used as a microbiological marker for water quality [31]. E. coli biosensors are one of the most commonly found in the literature, with various methods and electrodes used such as rotating disk electrodes [32], indium-doped tin oxide [33], glassy carbon electrodes (GCE) [34], and screen-printed electrodes (SPEs) [35]. For example, Kraazt et al [36] reported a label-free ferrocene-antimicrobial peptide (magainin I) modified gold electrode biosensor, showing its preferential selectivity towards pathogenic strains (O157:H7) with a LOD of 10 3 cfu/mL.…”

Section: Pathogen Detectionmentioning

confidence: 99%

Electroanalytical Overview: Electrochemical Sensing Platforms for Food and Drink Safety

2021

View full text Add to dashboard Cite

Robust, reliable, and affordable analytical techniques are essential for screening and monitoring food and water safety from contaminants, pathogens, and allergens that might be harmful upon consumption. Recent advances in decentralised, miniaturised, and rapid tests for health and environmental monitoring can provide an alternative solution to the classic laboratory-based analytical techniques currently utilised. Electrochemical biosensors offer a promising option as portable sensing platforms to expedite the transition from laboratory benchtop to on-site analysis. A plethora of electroanalytical sensor platforms have been produced for the detection of small molecules, proteins, and microorganisms vital to ensuring food and drink safety. These utilise various recognition systems, from direct electrochemical redox processes to biological recognition elements such as antibodies, enzymes, and aptamers; however, further exploration needs to be carried out, with many systems requiring validation against standard benchtop laboratory-based techniques to offer increased confidence in the sensing platforms. This short review demonstrates that electroanalytical biosensors already offer a sensitive, fast, and low-cost sensor platform for food and drink safety monitoring. With continued research into the development of these sensors, increased confidence in the safety of food and drink products for manufacturers, policy makers, and end users will result.

show abstract

Rapid electrochemical detection of Escherichia coli using nickel oxidation reaction on a rotating disk electrode

Cited by 36 publications

References 31 publications

Ultra-Fast Electrochemical Sensor for Point-of-Care COVID-19 Diagnosis Using Non-Invasive Saliva Sampling

Ultra-Fast Electrochemical Sensor for Point-of-Care COVID-19 Diagnosis Using Non-Invasive Saliva Sampling

On the application of 3d metals for C–H activation toward bioactive compounds: The key step for the synthesis of silver bullets

Electroanalytical Overview: Electrochemical Sensing Platforms for Food and Drink Safety

Contact Info

Product

Resources

About