Rapid electrochemical detection of MiRNA-21 facilitated by the excellent catalytic ability of Pt@CeO₂ nanospheres

Abstract

New Pt@CeO2 nanospheres with advanced catalytic ability were successfully applied to fabricate a highly sensitive electrochemical RNA sensor for rapid detection of miRNA-21 in real blood samples.

Nanomaterials promote the fast development of electrochemical MiRNA biosensors

“…Chen et al successfully developed an electrochemical biosensor with high sensitivity and specificity for the detection of miRNA-21 by fully using the catalytic ability of Pt@CeO 2 NSs to H 2 O 2 . 29 Using the target to trigger the reaction, the capture probe with the hairpin structure was opened, and the biotin at the end of the probe was activated. Through the biotin–avidin affinity interaction, Pt@CeO 2 NSs were successfully fixed on the electrode surface to catalyze H 2 O 2 to amplify the DPV signal.…”

“…Currently, more and more nanomaterials have been used to develop electrochemical sensors, including Au, 25 Pt, 26 carbon nanomaterials, 27 MoS 2 , 28 CeO 2 , 29 etc. , because of their unique properties, especially high specific surface area, which is good for the effective immobilization of biologically active molecules.…”

Electrochemical detection of miRNA‐21 based on molecular beacon formed by thymine‐mercury(II)‐thymine base pairing

“…These include optical detection methods such as colorimetric, fluorescence, surface plasmon resonance (SPR) [26] and surface-enhanced raman spectroscopy (SERS) [27], electrochemical and electrochemiluminescence (ECL) methods [28], inductively coupled plasma mass spectrometry (ICP-MS) [29], and piezoelectric detection methods such as surface acoustic wave (SAW) [30] and quartz crystal microbalance (QCM) technology [31]. Among these oligonucleotide (including miRNAs) detection methods, the electrochemical biosensor is the most studied method because of its many advantages; such as ease of use, small size, and low cost [32][33][34][35][36][37]. In general, most electrochemical biosensors obtain an electrochemical signal expressed as a current intensity for a hybridization event of an oligonucleotide (a specific nucleic acid) or specific antigen-antibody binding [38][39][40][41].…”

A concise overview of advancements in ultrasensitive biosensor development

“…Reported enhancements to sensor performance have been achieved through modifications involving various ligands, encompassing thionine ( Deng et al, 2018 ), Pd ( Gao and Yu, 2007b ; Wang et al, 2021a ), SA ( Hao et al, 2017 ), silver sulfide (Ag 2 S) ( Miao et al, 2016 ), iron oxides (Fe 2 O 3 /Fe 3 O 4 ) ( Yu et al, 2017 ; Zhou et al, 2017 ), cerium oxide (CeO2) ( Deng et al, 2018 ; Liu et al, 2018 ; Chen et al, 2022 ), titanium dioxide (TiO2), GO ( Erdem et al, 2017 ; Bao et al, 2019 ), MOFs ( Liang et al, 2019 ; Sun et al, 2020 ), PAn ( Peng et al, 2010 ; Wang et al, 2015b ) and MXenes ( Mohammadniaei et al, 2020 ). The enhancement of both LOD and miniaturization can be amplified by synergistically amalgamating diverse nanoparticle compositions and modification strategies that encompass ligands.…”