Rapid elicitor-induced chemiluminescence in soybean cell suspension cultures

Lindner, W.; Hoffmann, Christina; Grisebach, Hans

doi:10.1016/0031-9422(88)87017-1

Cited by 45 publications

(17 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also partially characterize the peroxidase and propose a role for it and the H202 burst in the overall disease resistance mechanism. A less extensive study leading to similar conclusions appeared in press during preparation of this manuscript (26).…”

supporting

confidence: 55%

Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells

Apostoł¹,

Heinstein²,

Low³

1989

Plant Physiol.

632

295

View full text Add to dashboard Cite

Stimulation of cultured plant cells with elicitors of the defense response leads to the rapid destruction of a variety of watersoluble compounds including indoleacetic acid and certain fluorescent dyes. This destructive activity, which is often vigorously manifested within 5 minutes of elicitor addition, is shown to derive from the rapid production of H202 and its use by extracellular peroxidases. Because of its speed of appearance, this oxidafive burst may qualify as the first induced line of defense against invading pathogens. Since H202 has been implicated as a second messenger of hormone-stimulated metabolic changes in some animal cells, its possible role in transduction of the defense signal in plants was also examined. Not only did exogenous H202 alone stimulate phytoalexin production in the plant cell suspension, but inhibition of elicitor-stimulated phytoalexin production was observed upon addition of catalase and other inhibitors of the oxidative burst. Furthermore, for inhibition to occur, the presence of catalase was required during elicitor addition, since if introduction of the enzyme was delayed until 1 hour after addition of the elicitor, no inhibition resulted. These results suggest that H202 also plays an important role in inducing subsequent defense responses such as phytoalexin production.

show abstract

supporting

confidence: 55%

Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells

Apostoł¹,

Heinstein²,

Low³

1989

Plant Physiol.

632

295

View full text Add to dashboard Cite

show abstract

“…Very early in this response, NADPH oxidase is activated, which releases O 2 . into the extracellular compartment (44 (45,46). Despite the importance of superoxide dismutase activity in this pathway, enzymes catalyzing this step have not yet been identified.…”

Section: Discussionmentioning

confidence: 99%

Tobacco Nectarin I

Carter

Thornburg

2000

Journal of Biological Chemistry

194

View full text Add to dashboard Cite

Nectarin I, a protein that accumulates in the nectar of Nicotiana sp., was determined to contain superoxide dismutase activity by colorimetric and in-gel assays. This activity was found to be remarkably thermostable. did not restore superoxide dismutase activity. The quaternary structure of the reconstituted enzyme was examined, and only tetrameric and pentameric aggregates were enzymatically active. The reconstituted enzyme was also shown to generate H 2 O 2 . Putative nectarin I homologues were found in the nectars of several other plant species.

show abstract

“…Although severa1 funga1 and plant cell-wall elicitors have been demonstrated to induce AO (Lindner et al, 1988;Aposto1 et al, 1989;Schwacke and Hager, 1992), harpin is the first bacterial protein associated with AO production. Based on this report and that of Wei et al (1992a), harpin should prove to be a useful cell-free tool to further investigate plant responses to incompatible bacteria.…”

Section: Resultsmentioning

confidence: 99%