Rapid Enantioseparation of 1-Aminoindan by Microchip Electrophoresis with Linear-Imaging UV Detection

Kitagawa, Fumihiko; Aizawa, Shiori; Otsuka, Koji

doi:10.2116/analsci.21.61

Cited by 37 publications

(14 citation statements)

References 56 publications

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“…A variety of channel dimensions have been used; however, the length of the detection area is set to 25 mm by the instrument configuration. The instrument was utilized in testing the applicability of transforming affinity CE (ACE) techniques to microchip format [50], in the rapid separation of antimicrobial metabolites with high efficiency [51], and in chiral separations of pharmaceuticals by CD EKC [52,53]. The same system was employed in developing a method to test for nitrogen monoxide metabolites in biological fluids [54], along with a single-step quantitation of DNA [55], and chip gel electrophoresis method for high-sensitivity DNA detection [56], as well as a monosaccharide derivative separation [57].…”

Section: Absorbance Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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Section: Absorbance Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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“…Prior to separation, 7 mL of the protein sample was introduced into the reservoir S and a platinum electrode was inserted into each reservoir to provide electrical contact. To introduce the sample solution into the separation channel, the pinched injection technique [30,31] was employed as shown in Fig. 1.…”

Section: Mce Analysis Of Proteinsmentioning

confidence: 99%

One-step immobilization of cationic polymer onto a poly(methyl methacrylate) microchip for high-performance electrophoretic analysis of proteins

Kitagawa

Kubota

Sueyoshi

et al. 2006

Science and Technology of Advanced Materials

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One-step covalent immobilization of poly(ethyleneimine) (PEI) onto poly(methyl methacrylate) (PMMA) substrates was investigated to achieve an efficient separation of basic proteins in microchip electrophoresis (MCE). The PEI-treated PMMA chip showed the anodic electroosmotic flow and its rate was almost kept stable during 32 days with over 50 runs. This longer stability of the prepared microchip indicated that the loss of PEI was successfully suppressed by the immobilization through the covalent bond. Furthermore, the PEI modification onto the PMMA chip could apparently reduce the surface adsorption of cationic proteins. In the MCE analysis on the PEImodified microchip, two proteins were successfully separated within 30 s only utilizing a separation length of 5 mm. While the migration time of the protein gradually increased during only four consecutive runs on an untreated PMMA chip, reproducible analyses were attained by using the PEI immobilized microchip. These results demonstrated that Coulombic repulsion force generated between cationic PEI and basic proteins could avoid the irreversible adsorption of the analytes onto the PMMA surface, which provided a highperformance analysis medium for biogenic compounds. r

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“…22 The linear-imaging UV detector allows for the MCE analysis of various compounds without any derivatization. 21,[23][24][25][26][27][28][29][30][31] Consequently, a UV detection scheme eliminates the labeling of sample proteins. Recently, Vlčková et al reported on the application of MCE-2010 to the MCIEF analysis of proteins on a conventional cross-channel chip.…”

Section: Introductionmentioning

confidence: 99%

High-speed Analysis of Proteins by Microchip Isoelectric Focusing with Linear-imaging UV Detection

2009

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High-speed analyses of proteins by microchip isoelectric focusing (MCIEF) were investigated on straight channel chips. By employing a commercially available microchip electrophoresis instrument equipped with a linear-imaging UV detector, sample proteins focused in a separation channel could be detected without a mobilization step. Typically, standard proteins were focused within 210 s at different positions in a separation channel with its total length of 38.8 mm on the basis of their pI values. The linear relationship between the focused position of each protein and its pI value was observed in the pH range from 6 to 10. The formation process of a pH gradient in the microchannel and the effects of the applied voltage and the channel length on the MCIEF separation were also investigated.

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Rapid Enantioseparation of 1-Aminoindan by Microchip Electrophoresis with Linear-Imaging UV Detection

Cited by 37 publications

References 56 publications

Unconventional detection methods for microfluidic devices

Unconventional detection methods for microfluidic devices

One-step immobilization of cationic polymer onto a poly(methyl methacrylate) microchip for high-performance electrophoretic analysis of proteins

High-speed Analysis of Proteins by Microchip Isoelectric Focusing with Linear-imaging UV Detection

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