Rapid equilibrium radioimmunoassay for the amino-terminal propeptide of human type III procollagen.

Risteli, Juha; Niemi, Seija; Trivedi, Premila; Mäentausta, O.; Mowat, Alex P.; Risteli, Leila

doi:10.1093/clinchem/34.4.715

Cited by 320 publications

(96 citation statements)

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“…We obtained a normal range for PICP of 50-210 g/l. The PIIINP assay detects the intact aminopropeptide and larger forms of PIIINP in serum, but not the smaller degradation products (Risteli et al, 1988). The normal reference range of PIIINP was 1 .…”

Section: Methodsmentioning

confidence: 97%

Serum markers of bone metabolism in multiple myeloma: prognostic value of the carboxy‐terminal telopeptide of type I collagen (ICTP)

Abildgaard¹,

Bentzen²,

Nielsen³

et al. 1997

Br J Haematol

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Summary. This study was performed to evaluate the prognostic significance of serum markers of bone and collagen metabolism in multiple myeloma. Serum C-terminal telopeptide of type I collagen (ICTP) reflects degradation of bone, whereas serum osteocalcin, together with serum Cterminal propeptide of procollagen type I (PICP) and serum bone-specific alkaline phosphatase (bAP) reflect synthesis of bone matrix. The N-terminal propeptide of procollagen type III (PIIINP) in serum reflects synthesis of type III collagen. We analysed frozen sera from 109 patients with newly diagnosed multiple myeloma. g/l). Serum PICP was generally within the reference limits, whereas serum osteocalcin and serum bAP were elevated in 19% and 37%, respectively. Serum ICTP correlated with serum PIIINP, serum 2 -microglobulin ( 2 m), serum calcium, performance status, and stage. In univariate analysis, the test variables serum ICTP (P 0 . 026) and serum osteocalcin (P 0 . 036) were found to be of prognostic value, but PIIINP, PICP, or bAP in serum were not. Serum ICTP and serum 2 m had a similar prognostic value. In multivariate analysis, serum calcium showed the highest prognostic significance, and serum 2 m was the only other variable of independent prognostic value. However, in normocalcaemic patients, serum ICTP showed the highest prognostic significance, followed by serum osteocalcin. Thus, the serum levels of ICTP and osteocalcin seem related to bone turnover and calcium metabolism, and provide further information about myeloma activity, particularly in normocalcaemic patients.

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Section: Methodsmentioning

confidence: 97%

Serum markers of bone metabolism in multiple myeloma: prognostic value of the carboxy‐terminal telopeptide of type I collagen (ICTP)

Abildgaard¹,

Bentzen²,

Nielsen³

et al. 1997

Br J Haematol

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“…Serum levels of PINP and PIIINP were analysed using RIAs. 8,9 TIMP-1, TIMP-2, MMP-9 and MMP-2/TIMP-2 complex in serum samples were detected using ELISA. Soluble PINP was also analysed from 4-mm skin biopsies.…”

Section: Biochemical Analysismentioning

confidence: 99%

Modulation of skin collagen metabolism by irradiation: collagen synthesis is increased in irradiated human skin

Riekki¹,

Jukkola

Sassi³

et al. 2000

British Journal of Dermatology

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Radiation-induced fibrosis is a common side-effect of cancer treatment. The pathophysiological events leading to fibrosis are not known in detail. We analysed the effect of therapeutic irradiation on human skin collagen synthesis, skin thickness, gelatinases and their inhibitors. Twenty randomly chosen women who had been treated for breast cancer with surgery and radiation therapy participated in the study. In each patient, the irradiated skin area was compared with a corresponding non-treated skin area. Suction blister fluid (SBF) and serum samples were analysed for the aminoterminal propeptides of type I and type III procollagens (PINP and PIIINP), tissue inhibitors of matrix metalloproteinases (MMPs) 1 and 2 (TIMP-1 and TIMP-2) and MMP-9 and MMP-2/TIMP-2 complex. Skin biopsies were analysed for PINP and immunohistochemical staining was used for PIIINP. In irradiated skin, PINP, PIIINP, TIMP-1 and MMP-2/TIMP-2 complex levels in SBF and the number of PINP-positive fibroblasts in tissue sections were significantly higher in comparison with non-treated skin. The levels of TIMP-2 in irradiated and non-irradiated skin were similar. MMP-9 could not be detected in SBF with the assay used. The serum levels of MMP-9 were higher in the treated subjects than the reference values. The serum values of PINP, PIIINP, TIMP-1, TIMP-2 and MMP-2/TIMP-2 complex were not significantly affected. These results indicate increased local collagen synthesis and accumulation of connective tissue in irradiated skin. The marked upregulation of collagen synthesis as a result of irradiation offers a possibility to treat this complication with compounds such as topical steroids which downregulate collagen synthesis.

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“…The intra-assay and interassay coefficients of variation for duplicate analyses were 1.5% (n = 10) and 1.7% (n = 10), respectively, at a 70HC concentration of 20 nM. Assay of serum collagen antigen content PIIINP concentration was measured by an equilibrium-type radioimmunoassay as described previously [12,13]. The method overcomes the problem of nonparallelism between the standard antigen and human samples encountered with commercial PIIINP radioimmunoassays.…”

Section: Assay Of Megx and 70hcmentioning

confidence: 99%

CYP3A4 and CYP2A6 activities marked by the metabolism of lignocaine and coumarin in patients with liver and kidney diseases and epileptic patients.

Rautio

Bäckström

et al. 1995

Brit J Clinical Pharma

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1. The in vitro hepatic metabolism of lignocaine to monoethylglycinexylide (MEGX) is mediated by CYP3A4 and that of coumarin to 7‐hydroxycoumarin (7OHC) by CYP2A6. We investigated the usefulness of monitoring serum MEGX concentrations (after 1 mg kg‐1 lignocaine i.v.) and urinary 7OHC excretion (after 5 mg coumarin p.o.) to reflect liver function in patients with liver (n = 36), kidney (n = 12) and epileptic (n = 12) disease and in control subjects (n = 20). The extent of liver disease was assessed using measurements of serum aminoterminal propeptide (PIIINP) and Child‐Pugh grades. 2. Serum concentrations of MEGX were decreased in severe (4.6 +/‐ 3.0 s.d. ng ml‐ 1), moderate (19.1 +/‐ 11.6 s.d. ng ml‐1) and mild (32.8 +/‐ 14.2 s.d ng ml‐1) liver disease as compared with controls (53.4 +/‐ 15.8 s.d ng ml‐1). The excretion of 7OHC over 2 h was decreased in severe (18.0 +/‐ 10.3 s.d % of dose) and moderate (34.2 +/‐ 15.6 s.d %), but not in mild (49.7 +/‐ 19.0 s.d %) liver disease as compared with that in controls (56.2 +/‐ 11.6%). 3. In epileptic patients the urinary recovery of 7OHC was increased (2 h value 69.5 +/‐ 13.2 s.d %) suggesting enzyme induction. In contrast, serum MEGX concentration were low (40.0 +/‐ 14.1 s.d ng ml‐1), possibly due to competition for CYP3A4 between lignocaine and antiepileptic drugs. 4. In patients with kidney disease serum MEGX concentration (56.5 +/‐ 26.1 s.d ng ml‐1) was similar to that in controls.(ABSTRACT TRUNCATED AT 250 WORDS)

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Rapid equilibrium radioimmunoassay for the amino-terminal propeptide of human type III procollagen.

Cited by 320 publications

References 0 publications

Serum markers of bone metabolism in multiple myeloma: prognostic value of the carboxy‐terminal telopeptide of type I collagen (ICTP)

Serum markers of bone metabolism in multiple myeloma: prognostic value of the carboxy‐terminal telopeptide of type I collagen (ICTP)

Modulation of skin collagen metabolism by irradiation: collagen synthesis is increased in irradiated human skin

CYP3A4 and CYP2A6 activities marked by the metabolism of lignocaine and coumarin in patients with liver and kidney diseases and epileptic patients.

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