Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales

Demord, Anthony; Poirel, Laurent; D'Emidio, Fernando; Pomponio, Stefano; Nordmann, Patrice

doi:10.1089/mdr.2020.0391

Cited by 14 publications

(5 citation statements)

References 11 publications

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“…The increasing incidence of MDR bacterial strains particularly for most common clinical pathogen P. aeruginosa in human isolates poses a significant challenge to identify new treatment strategies that can lead to high morbidity and mortality in hospitals. Fortunately, a number of rapid methods have been developed to identify such MDR species along with antibiotic susceptibility within a couple of hours from clinical isolates that have benefited to reduce increasing mortality [32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Multidrug Resistance Behaviors of Clinical Pseudomonas aeruginosa Strains Associated to Pigment Coloration

Kothari¹,

Yadav²,

Rajput³

et al. 2021

Preprint

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Pseudomonas aeruginosa is an adaptable bacterial pathogen that infects various organs, including the respiratory tract, vascular system, urinary tract, and central nervous system leading to high morbidity and mortality. Our primary focus of this study was to characterize P. aeruginosa clinical strains on the basis of pigment color production, determine its association to multidrug resistance behavior and ability to form biofilm. We identified yellow (30.1%), green (39.8%) and no pigment (30.1%) producing strains from a total of 143 clinical isolates. Yellow pigment producing strains presented significant resistance to a class of antibiotics including β-lactam (91.5%), aminoglycosides (70.5%), and carbapenems (51.9%) compared to green and non-pigmented strains. Importantly, 16.3% of yellow pigment producing strains was resistant to colistin where only 2.3% of non-pigmented and 1.8% of green pigmented strains were resistant to this agent. Moreover, yellow pigment producing strain were frequent producers of β-lactamase group of enzymes, ESBL (55.6%), MBL (55.6%), and AmpC (50%) and displayed higher frequency of efflux positive group (64.2%) compared to green (7.14%) and non-pigmented one (28.5%). Notably, green pigment producing strains when compared to non-pigmented groups also displayed antibiotic susceptibility behavior similar to yellow pigment producing strains. Although yellow pigment producing strains were strong biofilm producers, no significant association was identified between pigment and biofilm formation. Among pigmented and non-pigmented strains, majority of yellow pigment producing strains have shown MIC levels greater than the green and non-pigmented strains. Our study has demonstrated the impact of pigment coloration on susceptibility to antimicrobial agents where yellow pigment producing strains represent considerably a serious problem as due to lack of alternative agents against such transformed strain may collectively be associated with multidrug resistance development.

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Section: Discussionmentioning

confidence: 99%

Multidrug Resistance Behaviors of Clinical Pseudomonas aeruginosa Strains Associated to Pigment Coloration

Kothari¹,

Yadav²,

Rajput³

et al. 2021

Preprint

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“…The Rapid ESBL NP® (Liofilchem, Roseto degli Abbruzzi, Italy) is a colorimetric cefotaxime hydrolysis-based assay able to detect the presence/absence of any type of ESBLs or the presence of an enzyme or combination of enzymes that can hydrolyze cefotaxime, but which is not inhibited by the addition of tazobactam (i.e., cephalosporinase, ESBL + cephalosporinase, carbapenemase with or without an ESBL) [25,26]. Rapid ESBL NP® test was performed using the bacterial pellet recovered with MBT Sepsityper IVD Kit from 1 mL of positive BC broth, as previously described [11,12,27].…”

Section: Methodsmentioning

confidence: 99%

Direct detection of extended-spectrum-β-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis

Boattini

Bianco

Comini

et al. 2021

Eur J Clin Microbiol Infect Dis

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Accurate detection of extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales from bloodstream infection (BSI) is of paramount importance for both epidemiological and clinical purposes, especially for optimization of antibiotic stewardship interventions. Three phenotypic methods for the detection of ESBL phenotype in Klebsiella pneumoniae and Escherichia coli BSI were compared over a 4-month period (May-August 2021) in a main University Hospital from Northern Italy. The methods were the biochemical Rapid ESBL NP®, the immunological NG-Test CTX-M MULTI®, and the E-test technique based on ESBL E-test®. One hundred forty-two blood cultures (BCs) positive for K. pneumoniae or E. coli were included. ESBL and carbapenemase phenotype were detected in 26.1% (n = 37) and 16.9% (n = 24), respectively. The Rapid ESBL NP®, NG-Test CTX-M MULTI®, and direct ESBL E-test® positive and negative predictive values with 95% confidence intervals were 1 (0.87-1) and 0.97 (0.92-0.99), 1 (0.87-1) and 0.97 (0.92-0.99), and 1 (0.88-1) and 1 (0.96-1), respectively. The three phenotypic methods evaluated showed good performance in the detection of ESBL phenotype from K. pneumoniae-or E. coli-positive BCs. Rapid ESBL NP® and NG-test CTX-M® offer the important advantage of a turnaround time of 15 to 45 min, and the Rapid ESBL NP test in addition detects any type of ESBL producers.

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“…The constant change in the incidence of multidrug-resistance Gram-negative bacteria in patients with neutropenia requires rapid diagnostic tests for resistance identification; extended-spectrum B-lactamases and carbapenemases rapid identification, based on biochemical or fluorescence identification, have high sensitivities and specificities, are easy to perform, and provide results in 10–40 min [ 59 , 60 ]; prescribers will optimize antibiotics while waiting for the susceptibility test report.…”

Section: Ams Strategies To Optimize Antibiotic Therapymentioning

confidence: 99%

Antimicrobial Stewardship on Patients with Neutropenia: A Narrative Review Commissioned by Microorganisms

et al. 2023

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The emergence of antibiotic resistance poses a global health threat. High-risk patients such as those with neutropenia are particularly vulnerable to opportunistic infections, sepsis, and multidrug-resistant infections, and clinical outcomes remain the primary concern. Antimicrobial stewardship (AMS) programs should mainly focus on optimizing antibiotic use, decreasing adverse effects, and improving patient outcomes. There is a limited number of published studies assessing the impact of AMS programs on patients with neutropenia, where early appropriate antibiotic choice can be the difference between life and death. This narrative review updates the current advances in strategies of AMS for bacterial infections among high-risk patients with neutropenia. Diagnosis, drug, dose, duration, and de-escalation (5D) are the core variables among AMS strategies. Altered volumes of distribution can make standard dose regimens inadequate, and developing skills towards a personalized approach represents a major advance in therapy. Intensivists should partner antibiotic stewardship programs to improve patient care. Assembling multidisciplinary teams with trained and dedicated professionals for AMS is a priority.

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Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales

Cited by 14 publications

References 11 publications

Multidrug Resistance Behaviors of Clinical Pseudomonas aeruginosa Strains Associated to Pigment Coloration

Multidrug Resistance Behaviors of Clinical Pseudomonas aeruginosa Strains Associated to Pigment Coloration

Direct detection of extended-spectrum-β-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis

Antimicrobial Stewardship on Patients with Neutropenia: A Narrative Review Commissioned by Microorganisms

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