Growth kinetics were evaluated for three yeast strains of the genus Saccharomyces. Two topfloating strains, SF 115 and SF 116 and one flocculant yeast SF 104 were analyzed in pure and mixed cultures in 1-liter continuous fermentation experiments in a chemostat. Growth was monitored for 72 h at 30°C in a medium containing sugarbeet molasses and 1.0 g/liter each of NH4H,P0, and urea. SF 115 and SF 116 were found to have lowerpmax values of 0.290 and 0.296 h-l, respectively, than SF 104, which had a ,urnax of 0.364 h-l. The two top-floating yeasts (SF 115 and SF 116) demonstrated greater affinity for the substrate and utilized substrates a t a greater rate. They have K6 values of 4.03 x lW3 M and 3.798 x &I for SF 104.A mixed culture of SF 116 and SF 104 was found to have a pmax of 0.426 h-' with a K8 of 6.924 x 10-3 M. SF 115 grown in mixed culture with SF 104 exhibited a hmu of 0.473 h-l with a K, of 7.975 x 10-3 M. In both cases, the SF 104 was the dominant microbe in mixed culture systems.