Rapid Evaluation of Novel Therapeutic Strategies Using a 3D Collagen-Based Tissue-Like Model

Abstract

Graphical Abstract3D cell collagen-based model with tunable environment conditions (oxygen concentration, nanodrug exposure or irradiations).

Differential effects of biocompatible peritoneal dialysis fluids on human mesothelial and endothelial cells in 2D and 3D phenotypes

“…[15][16][17] The advantages of 3D cell cultures rely on the provision of an in vitro condition closer to the realistic one in terms of cell-cell and cellextracellular matrix interactions. 18 These interactions are very important in the PD context as the barrier function of PM is what makes the PD modality feasible. 19 Therefore in order to elucidate the effects of the PDF on the key barrierforming cell types in the context of PD, the mesothelial and the endothelial ones, a combined approach would be ideal.…”

“…However, a significant body of evidence suggests that the combined use of 2D and 3D cell cultures in the study of the biological behavior of cells under the influence of either stressors or testable drugs is far more informative 15–17 . The advantages of 3D cell cultures rely on the provision of an in vitro condition closer to the realistic one in terms of cell–cell and cell–extracellular matrix interactions 18 . These interactions are very important in the PD context as the barrier function of PM is what makes the PD modality feasible 19 .…”