Rapid Evaporative Ionisation Mass Spectrometry (REIMS) Provides Accurate Direct from Culture Species Identification within the Genus Candida

Cameron, Simon; Bolt, Frances; Perdones-Montero, Álvaro; Rickards, Tony; Hardiman, Kate; Abdolrasouli, Alireza; Burke, Adam; Bodai, Zsolt; Karancsi, Tamás; Simon, Daniel; Schaffer, Richard; Rebec, Monica; Balog, Júlia; Takáts, Zoltán

doi:10.1038/srep36788

Cited by 57 publications

(67 citation statements)

References 38 publications

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“…Initial applications of REIMS utilised electrosurgical hand-pieces, such as electrical diathermy surgical devices and bipolar surgical forceps, to direct the electrical current and aspirate the analyte-containing vapour to a mass spectrometer 20 . Recent advances have adapted REIMS to a modified liquid handling platform, which allows for the high-throughput (approximately 3-4,000 samples per 24 hour period) semi-automated analysis of samples with minimal user input 21,22 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Direct from Sample Metabolomics of Human Feces Using Rapid Evaporative Ionization Mass Spectrometry

et al. 2019

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Mass spectrometry is a powerful tool in the investigation of the human faecal metabolome. However, current approaches require time-consuming sample preparation, chromatographic separations, and consequently long analytical run times. Rapid evaporative ionisation mass spectrometry (REIMS) is a method of ambient ionisation mass spectrometry and has been utilised in the metabolic profiling of a diverse range of biological materials, including human tissue, cell culture lines, and microorganisms. Here, we describe the use of an automated, high-throughput REIMS robotic platform for direct analysis of human faeces. Through the analysis of faecal samples from five healthy male participants, REIMS analytical parameters were optimised and used to assess the chemical information obtainable using REIMS. Within the faecal samples analysed, bile acids, including primary, secondary, and conjugate species were identified, and phospholipids of possible bacterial origin were detected. In addition, the effect of storage conditions and consecutive freeze/thaw cycles was determined. Within the REIMS mass spectra, the lower molecular weight metabolites, such as fatty acids, were shown to be significantly affected by storage conditions for prolonged periods at temperatures above-80°C, and consecutive freeze/thaw cycles. However, the complex lipid region was shown to be unaffected by these conditions. A further cohort of 50 faecal samples, collected from patients undergoing bariatric surgery, were analysed using the optimised REIMS parameters, and the complex lipid region mass spectra used for multivariate modelling. This analysis showed a predicted separation between pre-and post-surgery specimens, suggesting that REIMS analysis can detect biological differences, such as microbiome-level differences, which have traditionally been reliant upon methods utilising extensive sample preparations and chromatographic separations and/or DNA sequencing.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Direct from Sample Metabolomics of Human Feces Using Rapid Evaporative Ionization Mass Spectrometry

et al. 2019

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“…Importantly, this high-throughput platform was shown to possess the same degree of analytical discrimination as the bipolar forceps REIMS approach, with substantially reduced intra-sample variability [ 10 ]. In addition, it was shown to be superior to the hand-held bipolar forceps in the species-level classification of eight Candida species, providing perfect accuracy [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Electrode Geometry on the Classification Performance of Rapid Evaporative Ionization Mass Spectrometric (REIMS) Bacterial Identification

Bodai

Cameron

Bolt

et al. 2017

J. Am. Soc. Mass Spectrom.

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The recently developed automated, high-throughput monopolar REIMS platform is suited for the identification of clinically important microorganisms. Although already comparable to the previously reported bipolar forceps method, optimization of the geometry of monopolar electrodes, at the heart of the system, holds the most scope for further improvements to be made. For this, sharp tip and round shaped electrodes were optimized to maximize species-level classification accuracy. Following optimization of the distance between the sample contact point and tube inlet with the sharp tip electrodes, the overall cross-validation accuracy improved from 77% to 93% in negative and from 33% to 63% in positive ion detection modes, compared with the original 4 mm distance electrode. As an alternative geometry, round tube shaped electrodes were developed. Geometry optimization of these included hole size, number, and position, which were also required to prevent plate pick-up due to vacuum formation. Additional features, namely a metal “X”-shaped insert and a pin in the middle were included to increase the contact surface with a microbial biomass to maximize aerosol production. Following optimization, cross-validation scores showed improvement in classification accuracy from 77% to 93% in negative and from 33% to 91% in positive ion detection modes. Supervised models were also built, and after the leave 20% out cross-validation, the overall classification accuracy was 98.5% in negative and 99% in positive ion detection modes. This suggests that the new generation of monopolar REIMS electrodes could provide substantially improved species level identification accuracies in both polarity detection modes. Graphical abstract Electronic supplementary materialThe online version of this article (10.1007/s13361-017-1818-5) contains supplementary material, which is available to authorized users.

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“…REIMS was also soon identified as a prospective tool for bacterial metabolomics (Strittmatter et al, 2013). The original work was extended to yeasts and fungi (Bolt et al, 2016;Cameron et al, 2016), although it is still being focused rather to a statistic approach and not to the actual structure identification of microbial metabolites (Strittmatter et al, 2014).…”

Section: Classification Of Small Molecules By Reimsmentioning

confidence: 99%

Meet interesting abbreviations in clinical mass spectrometry: from compound classification by REIMS to multimodal and mass spectrometry imaging (MSI)

Luptáková¹,

Pluháček²,

Palyzová³

et al. 2017

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This feature article discusses two modern mass spectrometry abbreviations in their clinical applications. Rapid evaporative ionization mass spectrometry (REIMS) is reported as a molecular classification tool useful for spectral features definition prior to mass spectrometry imaging (MSI). REIMS is appreciated not only as an ionization technique coupled with a surgical device but particularly as a biomarker discovery tool. For more complex understanding of pathological processes at cellular and molecular levels, the importance of multimodal approach in imaging applications is documented in the context of fiducial markers needed for hyperspectral data fusion collected by optical microscopy, elemental and molecular MSI. Finally, pathogen inactivation needed prior to the sectioning of the infected tissue is reported, and the impact of formaldehyde crosslinking to signal reduction is discussed.

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Rapid Evaporative Ionisation Mass Spectrometry (REIMS) Provides Accurate Direct from Culture Species Identification within the Genus Candida

Cited by 57 publications

References 38 publications

Evaluation of Direct from Sample Metabolomics of Human Feces Using Rapid Evaporative Ionization Mass Spectrometry

Evaluation of Direct from Sample Metabolomics of Human Feces Using Rapid Evaporative Ionization Mass Spectrometry

Effect of Electrode Geometry on the Classification Performance of Rapid Evaporative Ionization Mass Spectrometric (REIMS) Bacterial Identification

Meet interesting abbreviations in clinical mass spectrometry: from compound classification by REIMS to multimodal and mass spectrometry imaging (MSI)

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